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Effect of Several Herbicides on Dipeptidase Activity of Squash Cotyledons

Published online by Cambridge University Press:  12 June 2017

Ruey-Chyong Tsay  and
Floyd M. Ashton
Ruey-Chyong Tsay
Affiliation:
Dep. of Bot., Univ. of California, Davis, California 95616
Floyd M. Ashton
Affiliation:
Dep. of Bot., Univ. of California, Davis, California 95616
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Abstract

The dipeptidase activity of the cotyledons of the squash (Cucurbita maxima Duch. ‘Hubbard’) increases during germination. Several herbicides inhibited this increase in various degrees. Those herbicides inhibiting the development of dipeptidase activity 70% or more were (2,4-dichlorophenoxy)-acetic acid (2,4-D), 3,6-dichloro-o-anisic acid (dicamba), (2,3,6-trichlorophenyl)acetic acid (fenac), and N-1-naphthylphthalmic acid (naptalam). Those inhibiting dipeptidase activity by 63 to 34% were 4-amino-3,5,6-trichloropicolinic acid (picloram), 2,6-dichlorobenzonitrile (dichlobenil), 3,5-dibromo-4-hydroxybenzonitrile (bromoxynil), 3-amino-s-triazole (amitrole), 2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine (atrazine), 7-oxabicyclo[2.2.1] heptane-2,3-dicarboxylic acid (endothall), and 5-bromo-3-sec-butyl-6-methyluracil (bromacil). Those causing only slight inhibition, 24 to 16%, were N,N-dimethyl-2,2-diphenylacetamide (diphenamid), isopropyl m-chlorocarbanilate (chlorpropham), and α,α,α-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine (trifluralin); 3-(p-chlorophenyl)-1,1-dimethylurea (monuron) was essentially the same as the control. However, 2,2-dichloropropionic acid (dalapon), O,O-diisopropyl phosphorodithioate S-ester with N-(2-mercaptoethyl)-benzenesulfonamide (bensulide), N,N-diallyl-2-chloroacetamide (CDAA), and 2-chloroallyl diethyldithiocarbamate (CDEC) were somewhat stimulatory. When compared with previously reported herbicide activity on the proteinase activity, those herbicides which inhibited dipeptidase activity did not necessarily affect proteinase activity to the same degree.

Type
Research Article
Information
Weed Science , Volume 19 , Issue 6 , November 1971 , pp. 682 - 684
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

1. Ashton, F. M. and Dahmen, W. J. 1967. Purification and characterization of a dipeptidase from Cucurbita maxima cotyledons. Phytochemistry 6:1215–1225.Google Scholar
2. Ashton, F. M., Penner, D., and Hoffman, S. 1968. Effect of several herbicides on proteolytic activity of squash seedlings. Weed Sci. 16:169–171.Google Scholar
3. Foy, C. L. and Penner, D. 1965. Effect of inhibitors and herbicides on tricarboxylic acid cycle substrate oxidation by isolated cucumber mitochondria. Weeds 13:226–231.Google Scholar
4. Lotlikar, P. D., Remmert, L. F., and Freed, V. H. 1968. Effects of 2,4-D and other herbicides on oxidative phosphorylation in mitochondria from cabbage. Weed Sci. 16:161–165.Google Scholar
5. Mann, J. D., Jordan, L. S., and Day, D. E. 1965. A survey of herbicides for their effect upon protein synthesis. Plant Physiol. 40:840–843.CrossRefGoogle ScholarPubMed
6. Moreland, D. E., Malhotra, S. S., Gruenhagen, R. D., and Shokraii, E. H. 1969. Effects of herbicides on RNA and protein synthesis. Weed Sci. 17:556–563.Google Scholar
7. Penner, D. and Ashton, F. M. 1966. Proteolytic enzyme control in squash cotyledons. Nature 212:935–936.Google Scholar
8. Penner, D. and Ashton, F. M. 1967. Hormonal control of proteinase activity in squash cotyledon. Plant Physiol. 42:791–796.CrossRefGoogle Scholar
9. Penner, D. and Ashton, F. M. 1968. Influence of dichlobenil, endothal, and bromoxynil on kinin control of proteolytic activity. Weed Sci. 16:323–326.Google Scholar
10. Switzer, C. M. 1957. Effects of herbicides and related chemicals on oxidation and phosphorylation by isolated soybean mitochondria. Plant Physiol. 32:42–44.Google Scholar
11. Sze, H. and Ashton, F. M. 1971. Dipeptidase development in cotyledons of Cucurbita maxima during germination. Phytochemistry (in press).Google Scholar
12. Wiley, L. and Ashton, F. M. 1967. Influence of the embryonic axis on protein hydrolysis in cotyledons of Curcurbita maxima. Physiol. Plant. 20:688–696.Google Scholar