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Early Detection of Systemic Rust Infections of Dyers Woad (Isatis tinctoria) Using the Polymerase Chain Reaction

Published online by Cambridge University Press:  12 June 2017

Bradley R. Kropp ,
Steve Albee ,
Karen M. Flint ,
Paul Zambino ,
Les Szabo  and
Sherman V. Thomson
Bradley R. Kropp
Affiliation:
Dep. Biol., Utah State Univ., Logan, UT 84322
Steve Albee
Affiliation:
Dep. Biol., Utah State Univ., Logan, UT 84322
Karen M. Flint
Affiliation:
Dep. Biol., Utah State Univ., Logan, UT 84322
Paul Zambino
Affiliation:
Dep. Biol., Utah State Univ., Logan, UT 84322
Les Szabo
Affiliation:
USDA, ARS, Cereal Rust Lab. 151 Lindig St., St. Paul, MN 55108
Sherman V. Thomson
Affiliation:
USDA Forest Serv., Forestry Sci. Lab, 5985 Hwy K, P.O. Box 898, Rhinelander, WI 54501
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Abstract

Rust-specific polymerase chain reaction (PCR) primers selectively amplified ribosomal DNA of a rust fungus from infected dyers woad. PCR enabled DNA of the fungus to be detected in symptomatic plants as well as in asymptomatic parts of diseased plants. The use of PCR enabled early detection of rust infections in dyers woad plants during their first season when they are often asymptomatic Dried plant samples stored at room temperature for several months worked as well as lyophilized material for DNA extraction prior to PCR. The PCR detection method should greatly facilitate further studies on the biology and inoculation of this and other systemic rusts that have potential for use in biocontrol of weeds.

Keywords

Rust, Puccinia thlaspeos Schubdyers woad, Isatis tinctoria L. #3 ISATIBiological controlribosomal DNAmycoherbicide
Type
Special Topics
Information
Weed Science , Volume 43 , Issue 3 , September 1995 , pp. 467 - 472
Copyright
Copyright © 1995 by the Weed Science Society of America 

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References

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