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A cytochrome P450 monooxygenase cDNA (CYP71A10) confers resistance to linuron in transgenic Nicotiana tabacum

Published online by Cambridge University Press:  20 January 2017

Balazs Siminszky ,
Bonnie S. Sheldon ,
Frederick T. Corbin  and
Ralph E. Dewey
Bonnie S. Sheldon
Affiliation:
Department of Crop Science, North Carolina State University, Raleigh, NC 27695-7620
Frederick T. Corbin
Affiliation:
Department of Crop Science, North Carolina State University, Raleigh, NC 27695-7620
Ralph E. Dewey
Affiliation:
Department of Crop Science, North Carolina State University, Raleigh, NC 27695-7620
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Abstract

The isolation of a Glycine max cytochrome P450 monooxygenase (P450) cDNA designated CYP71A10 that conferred linuron resistance to laboratory-grown, transgenic Nicotiana tabacum seedlings was previously reported. A nonsegregating transgenic N. tabacum line has been established that possesses two independent copies of the G. max CYP71A10 transgene. Five-week-old progeny plants of this selected line were grown in a controlled environmental chamber and treated with linuron using either pretransplant incorporated (PTI) or postemergence (POST) applications. CYP71A10-transformed N. tabacum was more tolerant to linuron than the wild type for both application methods. The transgenic N. tabacum line tolerated an approximately 16-fold and 12-fold higher rate of linuron than wild-type N. tabacum when the herbicide was applied PTI or POST, respectively. These results provide evidence that plant-derived P450 genes can be employed effectively to confer herbicide resistance to transgenic plants.

Keywords

Cytochrome P450linuronGlycine max L. Merr. ‘Dare’, soybeanNicotiana tabacum L. ‘SR1’, tobaccoHerbicide metabolismphenylureagenetic engineeringmetabolic engineering
Type
Research Article
Information
Weed Science , Volume 48 , Issue 3 , June 2000 , pp. 291 - 295
Copyright
Copyright © Weed Science Society of America 

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