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Cloning, characterization, and expression of growth regulator CYCLIN D3-2 in leafy spurge (Euphorbia esula)

Published online by Cambridge University Press:  20 January 2017

David P. Horvath ,
James V. Anderson ,
Ying Jia  and
Wun S. Chao
James V. Anderson
Affiliation:
USDA–ARS–RRVARC, 1605 Albrecht Boulevard, Fargo, ND 58105
Ying Jia
Affiliation:
USDA–ARS–RRVARC, 1605 Albrecht Boulevard, Fargo, ND 58105
Wun S. Chao
Affiliation:
USDA–ARS–RRVARC, 1605 Albrecht Boulevard, Fargo, ND 58105
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Abstract

We have isolated both a genomic and near full length cDNA clone for a D-class cyclin gene from the perennial weed leafy spurge. Sequence analysis indicates that this gene has the highest similarity to CYCLIN D3-2 of Arabidopsis. This gene is preferentially expressed in growing shoot apices and is up-regulated in adventitious buds on resumption of growth following loss of correlative inhibition (apical dominance). CYCLIN D3-2 is also induced in nongrowing adventitious buds of plants treated with gibberellic acid or after removal of leaves—treatments known to up-regulate expression of G1 to S phase transition–specific genes such as HISTONE H3 in adventitious buds. CYCLIN D3-2 was not induced on removal of the apical and axillary buds. Expression of CYCLIN D3-2 is down-regulated in adventitious crown buds during initiation of ecodormancy in early winter. Sequence comparisons of CYCLIN D3-2 with its putative orthologue from Arabidopsis identified several conserved motifs in the promoter region and a conserved region capable of forming a stable hairpin loop in the 5′ untranslated region. Conservation of these noncoding sequences across species strongly suggests they have a regulatory function.

Keywords

Leafy spurge, Euphorbia esula L. EPHESarabidopsis Arabidopsis thaliana L. ARATHBud dormancycell cyclegrowthcyclins
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 53 , Issue 4 , August 2005 , pp. 431 - 437
Copyright
Copyright © Weed Science Society of America 

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