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An Ultrastructural Study of the Effects of 2,4-D on Tobacco Leaves

Published online by Cambridge University Press:  12 June 2017

J. A. White  and
D. D. Hemphill
J. A. White
Affiliation:
Electron Microscope Laboratory, Dep. of Plant Path., Univ. of Mo.—Columbia, Columbia, Missouri, 65201
D. D. Hemphill
Affiliation:
Univ. of Mo.—Columbia, Columbia, Missouri, 65201
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Abstract

Tobacco (Nicotiana tabacum L. ‘Samsun N. N.’) leaves were treated with (2,4-dichlorophenoxy)acetic acid (2,4-D) at a concentration of 500 mg/L by the syringe infiltration technique and then sampled at regular intervals. Electron microscopic examination of the treated tissue revealed that the response of the mesophyll cells to 2,4-D was related to the age of the leaf at the time of treatment. Expanding leaves from the upper portion of the plant exhibited a high degree of tolerance to 2,4-D. Mature, fully-expanded leaves from the lower part of the plant were highly sensitive to the herbicide, with the mesophyll cells undergoing rapid structural breakdown. This breakdown involved a rupturing and disintegration of the tonoplast, plasmalemma, and membranes of chloroplasts and mitochondria.

Type
Research Article
Information
Weed Science , Volume 20 , Issue 5 , September 1972 , pp. 478 - 481
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

1. Audus, L. J. 1964. The physiology and biochemistry of herbicides. Academic Press, New York. 555 p.Google Scholar
2. Bachelard, E. P. and Ayling, R. D., 1971. The effects of picloram and 2,4-D on plant cell membranes. Weed Res. 11:31–36.Google Scholar
3. Baxter, R. and Hanson, J. B. 1968. The effect of 2,4-dichlorophenoxyacetic acid upon the metabolism and composition of soybean hypocotyl mitochondria. Planta 82:246–260.CrossRefGoogle Scholar
4. Chrispeels, M. J. and Hanson, J. B. 1962. The increase in ribonucleic acid content of cytoplasmic particulates of soybean hypocotyl induced by 2,4-D. Weeds 10:123–125.CrossRefGoogle Scholar
5. Freed, V. H., Reithel, F. J., and Remmert, L. F. 1961. Some physical-chemical aspects of synthetic auxins with respect to their mode of action. p. 289–306. In Plant Growth Regulation, Proc. of Fourth Int. Conf. Iowa State University Press, Ames, Iowa.Google Scholar
6. Galston, A. W. and Kaur, R. 1961. The intracellular locale of auxin action: an effect of auxin on the physical state of cytoplasmic proteins. p. 355–362. In Plant Growth Regulation, Proc. of Fourth Int. Conf. Iowa State University Press, Ames, Iowa.Google Scholar
7. Hallam, N. D. 1970. The effect of 2,4-D and related compounds on the fine structure of the primary leaves of Phaseolus vulgaris . J. Exp. Bot. 21:1031–1038.Google Scholar
8. Key, J. L., Hanson, J. B., and Bils, R. F. 1960. Effect of 2,4-dichlorophenoxyacetic acid application on activity and composition of mitochondria from soybeans. Plant Physiol. 35:177–183.Google Scholar
9. Marinos, N. G. 1957. Responses of Avena coleoptile sections to high concentrations of auxin. Aust. J. Biol. Sci. 10:147–163.CrossRefGoogle Scholar
10. Mashtakov, S. W., Deeva, V. P., and Volynets, A. P. 1967. Physiological effect of herbicides upon cultivated plant varieties. Nauka i tekhnika. Minsk. 193 p.Google Scholar
11. Wareing, P. F. and Phillips, I. D. J. 1970. The control of growth and differentiation in plants. Pergamon Press, New York. 303 p.Google Scholar