Hostname: page-component-cd9895bd7-gbm5v Total loading time: 0 Render date: 2024-12-24T19:52:15.155Z Has data issue: false hasContentIssue false

An Inexpensive Combustion Apparatus for Preparation of Radioactive Samples

Published online by Cambridge University Press:  12 June 2017

Dennis L. Bucholtz
Affiliation:
Dep. Bot. Plant Path., Purdue Univ., West Lafayette, IN 47907
F. Dan Hess
Affiliation:
Dep. Bot. Plant Path., Purdue Univ., West Lafayette, IN 47907

Abstract

The combustion apparatus described provides a rapid and inexpensive alternative for measurement of radioisotopes in plant parts. The apparatus utilized a nickel-chromium resistance wire connected to a low-voltage transformer to generate the heat required to ignite the plant tissue. The nickel-chromium wire also served as the sample holder. Combustion was performed in an O2-flushed liquid scintillation vial, and the combustion gases containing either 14CO2 or 3H2O were trapped in situ, scintillant was added, and the vials assayed for radioactivity. The percentage of the radioactivity recovered after combustion was 96.0 for 14C and 93.4 for 3H with standard deviations of 6.8 and 3.0%, respectively.

Type
Research Article
Copyright
Copyright © 1983 Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Barrow, A. and Griffiths, L. A. 1976. Liquid scintillation counting of aqueous solutions of potassium salts in a Triton X-100/toluene scintillant. Anal. Biochem. 74:246250.CrossRefGoogle Scholar
2. Duncombe, W. G. and Rising, T. J. 1969. Scintillation counting of 14CO2 from in vitro systems: a comparison of some trapping agents. Anal. Biochem. 30:275278.CrossRefGoogle ScholarPubMed
3. Gupta, G. N. 1966. A simple in-vial combustion method for assay of hydrogen-3, carbon-14, and sulfur-35 in biological, biochemical, and organic materials. Anal. Chem. 38:13561359.CrossRefGoogle Scholar
4. Kelly, R. G., Peets, E. A., Gordon, S., and Buyske, D. A. 1961. Determination of C14 and H3 in biological samples by Schöniger combustion and liquid scintillation techniques. Anal. Biochem. 2:267273.CrossRefGoogle Scholar
5. Knoche, H. W. and Bell, R. M. 1965. Tritium assay by combustion with a novel oxygen train and liquid scintillation techniques. Anal. Biochem. 12:4959.Google Scholar
6. Peterson, J. I. 1969. A carbon dioxide collection accessory for the rapid combustion apparatus for preparation of biological samples for liquid scintillation analysis. Anal. Biochem. 31:204210.CrossRefGoogle Scholar
7. Peterson, J. I., Wagner, F., Siegel, S., and Nixon, W. 1969. A system for convenient combustion preparation of tritiated biological samples for scintillation analysis. Anal. Biochem. 31:189203.CrossRefGoogle ScholarPubMed
8. Umbreit, W. W., Burris, R. H., and Stauffer, J. F. 1945. Manometric Techniques and Related Methods for the Study of Tissue Metabolism. Burgess Publishing Co., Minneapolis, MN. 198.Google Scholar