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An Automated System for Large-Scale Recovery of Germination Stimulants and Other Root Exudates

Published online by Cambridge University Press:  12 June 2017

Sigrid Müller ,
Andre Van Der Merwe ,
Hermann Schildknecht  and
Johann H. Visser
Sigrid Müller
Affiliation:
Chemistry Dep., Univ. Missouri, Columbia, MO 65211
Andre Van Der Merwe
Affiliation:
Dep. Bot., Univ. Stellenbosch, 7600 Stellenbosch, South-Africa
Hermann Schildknecht
Affiliation:
Org. Chemisches Institut, Im Neuenheimer Feld 270, 6900 Heidelberg, Germany
Johann H. Visser
Affiliation:
Dep. Bot., Univ. Stellenbosch, 7600 Stellenbosch, South-Africa
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Abstract

A large-scale continuous system for recovery of root exudates from millet and cowpea was developed and tested extensively. Large quantities of germination stimulants were exuded into the medium flowing through the plant trays, which could be demonstrated by germination of seeds of three species of angiospermous parasitic plants, Alectra vogelii, Striga gesneriodes, and witchweed. Recovery of root exudates was affected by adsorption on the macroreticular polymer resin XAD-4 followed by desorption with methanol. Furthermore, indications were found that germination inhibitors are released into the medium. Subsequent testing confirmed the quantitative release of the germination stimulants as well as a large number of other compounds.

Keywords

Alectra vogelii Benthcowpea, Vigna unguiculata (L.) Walp. ‘Saunders Upright’millet, Sorghum vulgare (L.) ‘Haygrazer’Striga gesnerioides (Willd.) Vatke #3 STRGEwitchweed, Striga asiatica (L.) Kuntze # STRLURoot exudategermination stimulantsgermination inhibitors
Type
Special Topics
Information
Weed Science , Volume 41 , Issue 1 , March 1993 , pp. 138 - 143
Copyright
Copyright © 1993 by the Weed Science Society of America 

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References

Literature Cited

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