Hostname: page-component-cd9895bd7-hc48f Total loading time: 0 Render date: 2024-12-25T17:13:29.794Z Has data issue: false hasContentIssue false
  • English
  • Français

ALS-Resistant Smallflower Umbrella Sedge (Cyperus difformis) in Arkansas Rice: Physiological and Molecular Basis of Resistance

Published online by Cambridge University Press:  20 January 2017

Parsa Tehranchian ,
Dilpreet S. Riar ,
Jason K. Norsworthy ,
Vijay Nandula ,
Scott McElroy ,
Shu Chen  and
Robert C. Scott
Parsa Tehranchian*
Affiliation:
Department of Crop, Soil, and Environmental Sciences, University of Arkansas, 1366 West Altheimer Drive, Fayetteville, AR 72704
Dilpreet S. Riar
Affiliation:
Department of Crop, Soil, and Environmental Sciences, University of Arkansas, 1366 West Altheimer Drive, Fayetteville, AR 72704
Jason K. Norsworthy
Affiliation:
Department of Crop, Soil, and Environmental Sciences, University of Arkansas, 1366 West Altheimer Drive, Fayetteville, AR 72704
Vijay Nandula
Affiliation:
Crop Production Systems Research Unit, United States Department of Agriculture, Agricultural Research Services, 141 Experiment Station Road, P.O. Box 350, Stoneville, MS 38776
Scott McElroy
Affiliation:
Department of Crop, Soil, and Environmental Sciences, Auburn University, 201 Funchess Hall, Auburn, AL 36849
Shu Chen
Affiliation:
Department of Crop, Soil, and Environmental Sciences, Auburn University, 201 Funchess Hall, Auburn, AL 36849
Robert C. Scott
Affiliation:
Department of Crop, Soil, and Environmental Sciences, University of Arkansas, Box 357, Lonoke, AR 72086
*
Corresponding author's E-mail: [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Smallflower umbrella sedge is a problematic weed in direct-seeded rice in the midsouthern United States. It recently has evolved resistance to the acetolactate synthase (ALS) –inhibiting herbicide halosulfuron in Arkansas rice. Studies were conducted (1) to determine if the resistant biotype is cross resistant to other ALS-inhibiting herbicides, (2) to evaluate alternative herbicide control options, and (3) to determine the mechanism of resistance. Whole-plant bioassay revealed that halosulfuron-resistant plants were not controlled by bispyribac–sodium, imazamox, and penoxsulam at the labeled field rate of each herbicide. The level of resistance to these herbicides, based on the lethal dose needed to kill 50% of plants (LD50) was ≥ 15-fold compared to a susceptible biotype. Both biotypes were controlled >96% with bentazon and propanil and ≤ 23% with quinclorac, thiobencarb, and 2,4-D. Hence, effective control measures exist; albeit, the number of herbicide options appear limited. Based on in vitro ALS enzyme assays, altered target site is the mechanism of resistance to halosulfuron and imazamox. Massively parallel sequencing with the use of the Illumina HiSeq detected an amino acid substitution of Pro197-to-His in the resistant biotype that is consistent with ALS-inhibiting herbicide resistance in other weed species.

Keywords

Bispyribace–sodiumhalosulfuronimazamoximazethapyrpenoxsulamsmallflower umbrella sedge, Cyperus difformis L.rice, Oryza sativa L.ALS enzyme assayALS gene sequencingherbicide resistance mechanismIllumina HiSeqwhole-plant bioassay
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 63 , Issue 3 , September 2015 , pp. 561 - 568
Creative Commons
Creative Common License - CCCreative Common License - BY
This is an Open Access article, distributed under the terms of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright
Copyright © Weed Science Society of America

References

Literature Cited

Baltazar, AM, Smith, RJ Jr. (1994) Propanil-resistant barnyardgrass (Echinochloa crus-galli) control in rice (Oryza sativa). Weed Sci. 8:576–581 Google Scholar
Bräutigam, A, Gowik, U (2010) What can next generation sequencing do for you? Next generation sequencing as a valuable tool in plant research. Plant Biol. 12:831–841 Google Scholar
Busi, R, Vidotto, F, Fischer, AJ, Osuna, MD, De Prado, R, Ferrero, A (2006) Patterns of resistance to ALS herbicides in smallflower umbrella sedge (Cyperus difformis) and ricefield bulrush (Schoenoplectus mucronatus). Weed Technol. 20:1004–1014 Google Scholar
Galon, L, Panozzo, LE, Noldin, JA, Concenço, G, Tarouco, CP, Ferreira, EA, Agostinetto, D, Silva, AA, Ferreira, FA (2008) Herbicide resistance of Cyperus difformis to ALS-inhibitors in paddy rice of Santa Catarina. Planta Daninha. 26:419–427 Google Scholar
Graham, RJ, Pratley, JE, Slater, PD, Baines, PR (1996) Herbicide resistant aquatic weeds, a problem in New South Wales rice crops. Pp 156–158 in Sheperd RCH, ed. Proceedings of the 11th Australian Weeds Conference, Melbourne, Australia. Parkville, Victoria, Australia Weed Science Society of Victoria Google Scholar
Haas, BJ, et al. 2013. De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis. Nature Prot. 8:1494–1512 Google Scholar
Heap, I (2014) The International Survey of Herbicide Resistant Weeds. http://www.weedscience.com. Accessed September 24, 2014Google Scholar
Kuk, YI, Kim, KH, Kwon, OD, Lee, DJ, Burgos, NR, Jung, S, Guh, JO (2004) Cross-resistance pattern and alternative herbicides for Cyperus difformis resistant to sulfonylurea herbicides in Korea. Pest Manag Sci. 60:85–94 Google Scholar
Lovelace, ML (2003) Implications of quinclorac use in Arkansas: impacts of quinclorac drift on tomato physiology and development of quinclorac resistance in barnyardgrass. Ph.D Dissertation. Fayetteville, AR University of Arkansas. Pp 70–71 Google Scholar
McWilliam, H, Li, W, Uludag, M, Squizzato, S, Park, YM, Buso, N, Cowley, AP, Lopez, R (2013) Analysis tools web services from the EMBL-EBI. Nucl Acids Res. 41:W597–W600 Google Scholar
Merotto, A Jr., Jasieniuk, M, Osuna, MD, Vidotto, F, Ferrero, A, Fischer, AJ (2009) Cross-resistance to herbicides of five ALS-inhibiting groups and sequencing of the ALS gene in Cyperus difformis L. J Agric Food Chem. 57:1389–1398 Google Scholar
Nandula, VK, Messersmith, CG (2000) Mechanism of wild oat (Avena fatua L.) resistance to imazamethabenz-methyl. Pestic Biochem Physiol. 68:148–155 Google Scholar
Norsworthy, JK, Bond, J, Scott, RC (2013a) Weed management practices and needs in Arkansas and Mississippi rice. Weed Technol. 27:623–630 Google Scholar
Norsworthy, JK, Riar, DS, Scott, RC (2013b) Control options for ALS-resistant smallflower umbrella sedge in Arkansas rice. Pp 222–226 in Norman RJ, Meullenet JF, Moldenhauer KAK, eds. B.R. Wells Arkansas Rice Research Studies. Fayetteville, AR University of Arkansas Agricultural Experiment Station Research series 617Google Scholar
Norsworthy, JK, Scott, R, Smith, K, Still, J, Estorninos, LE Jr., and Bangarwa, S (2009) Confirmation and management of clomazone-resistant barnyardgrass in rice. Proc South Weed Sci Soc. 62: 210 [Abstract]Google Scholar
Osuna, MD, Vidotto, F, Fischer, AJ, Bayer, DE, De Prado, R, Ferrero, A (2002) Cross resistance to bispyribac–sodium and bensulfuron-methyl in Echinochloa phyllopogon and Cyperus difformis . Pestic Biochem Physiol. 73:9–17 Google Scholar
Pedroso, RM, Boddy, LG, Fischer, A (2013) Propanil resistance in smallflower umbrella sedge (Cyperus difformis L.): a new challenge to rice growers in California. http://wssaabstracts.com/public/17/acrebstract-11.html. Accessed February 11, 2014Google Scholar
Powles, SB, Yu, Q (2010) Evolution in action: plants resistant to herbicides. Annu Rev Plant Biol. 61:317–347 Google Scholar
Riar, DS, Norsworthy, JK, Bond, JA, Bararpour, MT, Wilson, MJ, Scott, RC (2012) Resistance of Echinochloa crus-galli populations to acetolactate synthase-inhibiting herbicides. Int J Agron. 2012:893–953 Google Scholar
Riar, DS, Norsworthy, JK, Srivastava, V, Nandula, V, Bond, JA, Scott, RC (2013) Physiological and molecular basis of acetolactate synthase-inhibiting herbicide resistance in barnyardgrass (Echinochloa crus-galli). J Agric Food Chem. 61:278–289 Google Scholar
Ruiz-Santaella, JP, Bakkaliu, Y, Osuna, MD, De Prado, R (2004) Evaluation of resistance in Cyperus difformis populations to ALS inhibiting herbicides. Comm Agri Appl Biol Sci. 69:91–96 Google Scholar
Scott, RC, Boyd, JW, Smith, KL, Selden, G, Norsworthy, JK (2013) Recommended Chemicals for Weed and Brush Control. University of Arkansas Division of Agriculture, Cooperative Extension Service, Miscellaneous Publication 44. 36 p Google Scholar
Siminszky, B (2006) Plant cytochrome P450-mediated herbicide metabolism. Phytochem Rev. 5:445–458 Google Scholar
Tranel, PJ, Wright, TR (2002) Resistance of weeds to ALS-inhibiting herbicides: what have we learned? Weed Sci. 50:700–712 Google Scholar
Tranel, PJ, Wright, TR, Heap, IM (2014) Mutations in herbicide-resistant weeds to ALS inhibitors. http://weedscience.com. Accessed September 24, 2014Google Scholar
Vidotto, F, Busi, R, Ferrero, A (2003) Schoenoplectus mucronatus (L.) Palla and Cyperus difformis L. accessions resistant to ALS-inhibitors in Italian rice fields. in Proceedings of the Third International Temperate Rice Conference. Unpaginated CDGoogle Scholar
Wilson, MJ, Norsworthy, JK, Johnson, DB, McCallister, EK, Devore, JD, Griffith, GM, Bangarwa, SK, Scott, RC, Smith, KL (2009) Herbicide programs for controlling ALS-resistant barnyardgrass in Arkansas rice. Pages 153–157 in Norman, RJ, Meullenet, JF, Moldenhauer, KAK, eds. B.R. Wells Arkansas Rice Research Studies. Fayetteville, AR: University of Arkansas Agricultural Experiment Station Research series 581.Google Scholar
Yasuor, H, Osuna, MD, Ortiz, A, Saldaín, NE, Eckert, JW, Fischer, AJ (2009) Mechanism of resistance to penoxsulam in late watergrass [Echinochloa phyllopogon (Stapf) Koss.]. Agric Food Chem. 57:3653–3660 Google Scholar
Yuan, JS, Tranel, PJ, Stewart, CN. Jr. (2007) Non-target-site herbicide resistance: a family business. Trends Plant Sci. 12:6–13 Google Scholar