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Adjuvant-Increased Glyphosate Uptake by Protoplasts Isolated from Quackgrass Elytrigia repens (L.) Nevski

Published online by Cambridge University Press:  12 June 2017

Hans De Ruiter  and
Esther Meinen
Hans De Ruiter
Affiliation:
DLO Research Institute for Agrobiology and Soil Fertility, P.O. Box 14, 6700 AA Wageningen, The Netherlands
Esther Meinen
Affiliation:
DLO Research Institute for Agrobiology and Soil Fertility, P.O. Box 14, 6700 AA Wageningen, The Netherlands
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Abstract

Protoplasts were isolated from quackgrass to investigate the influence of two surfactants and ammonium sulfate on glyphosate uptake. Without adjuvants, the uptake of glyphosate was not detectable. The uptake of the reference compound 2-aminoisobutyric acid was linear with time and was inhibited 60% in the presence of 10 μM carbonyl cyanide m-chlorophenylhydrazone. Glyphosate uptake increased in the presence of both surfactants and ammonium sulfate. After 0.5 h of incubation the internal glyphosate concentration was 37% of the external concentration (100 μM) in the presence of 0.025 g L−1 Ethomeen T/25, 21% in the presence of 0.25 g L−1 Atplus 201, 13% with 5 mM ammonium sulfate, and 20% with 50 mM ammonium sulfate. The surfactant treatments did not reduce either the viability or the internal pH (= 7.1) as judged by fluorescein diacetate staining or 5,5-dimethyloxazolidine-2,4-dione distribution, respectively. The treatments with ammonium sulfate slightly increased the internal pH. The concentration of Ethomeen T/25 required to disrupt the protoplasts was 0.075 g L−1; Atplus 201 did not disrupt the protoplasts at concentrations up to 10 g L−1.

Keywords

Glyphosate, N-(phosphonomethylglycine)Atplus 201 (polyoxyethylene (20) hexitan monolaurate ester)Ethomeen T/25 (polyoxyethylene (15) tallow amine)quackgrass, Elytrigia repens (L.) Nevski ♯ AGRRESurfactantammonium sulfate2-aminoisobutyric acidcell membrane
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 44 , Issue 1 , March 1996 , pp. 38 - 45
Copyright
Copyright © 1996 by the Weed Science Society of America 

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