Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-11-28T02:14:23.410Z Has data issue: false hasContentIssue false
  • English
  • Français

A rapid in vivo shikimate accumulation assay with excised leaf discs

Published online by Cambridge University Press:  20 January 2017

Dale L. Shaner ,
Talia Nadler-Hassar ,
W. Brien Henry  and
Clifford H. Koger
Talia Nadler-Hassar
Affiliation:
Colorado State University, Biological Science and Pest Management, Weed Science Lab, Fort Collins, CO 80527
W. Brien Henry
Affiliation:
USDA-ARS, Central Plains Resources Management Research Unit, 40335 County Road GG, Akron, CO 80720
Clifford H. Koger
Affiliation:
USDA-ARS, Southern Weed Science Research Unit, P.O. Box 350, Stoneville, MS 38776
Get access Rights & Permissions [Opens in a new window]

Abstract

An in vivo shikimate accumulation assay with excised leaf tissue was developed to provide a fast and reliable method for identifying glyphosate-resistant plants. The assay is based on glyphosate-induced accumulation of shikimate. There was a linear accumulation of shikimate in excised leaf discs of soybean and canola treated with 250 μM glyphosate for 48 h. The IC50 for the accumulation of shikimate in soybean and corn leaf discs was 34 and 87 μM, respectively. Leaf discs excised from glyphosate-resistant corn or soybean did not accumulate shikimate when treated with 500 μM glyphosate. Leaf discs taken from a number of field-grown plants accumulated shikimate in a glyphosate dose–dependent manner. The accumulation of shikimate was dependent on light and the age of the leaf from which the disc was taken. The assay worked either in 96-well microtiter plates or in vials, and it clearly differentiated between glyphosate-resistant and -susceptible crops in which the resistance is due to an alteration of the target site for glyphosate. The assay was simple and robust and has the potential to be used as a high throughput assay to detect glyphosate resistance in weeds.

Keywords

Glyphosatecanola, Brassica napus L. ‘Hayola 420’corn, Zea mays L. ‘Pioneer 37M34’, ‘Dekalb DK493RR/BTY’soybean, Glycine max (L.) Merr., ‘Asgrow A2869’, ‘Asgrow AG3003’Glyphosate resistanceresistance detection
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 53 , Issue 6 , December 2005 , pp. 769 - 774
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

Amrhein, N., Deus, B., Gehrke, P., and Steinrucken, H. C. 1980. The site of inhibition of the shikimate pathway by glyphosate. II. Interference of glyphosate with chorismate formation in vivo and in vitro. Plant Physiol 66:830–834.Google Scholar
Arnaud, L., Nurit, F., Ravanel, P., and Tissut, M. 1994. Distribution of glyphosate and of its target enzyme inside wheat plants. Pestic. Sci 40:217–223.CrossRefGoogle Scholar
Baerson, S. R., Rodriguez, D. J., Biest, N. A., Tran, M., You, J., Krueger, R. W., Dill, G. R., Pratley, J. E., and Gruys, K. J. 2000. Investigating the mechanism of glyphosate resistance in rigid ryegrass (Lolium rigidum). Weed Sci 50:721–730.Google Scholar
Baerson, S. R., Rodriguez, D. J., Tan, M., Feng, Y., Biest, N. A., and Dill, G. M. 2002. Glyphosate-resistant goosegrass. Identification of a mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase. Plant Physiol 129:1265–1275.Google Scholar
Beckie, H. J., Heap, I. M., Smeda, R. J., and Hall, L. M. 2000. Screening for herbicide resistance in weeds. Weed Technol 14:428–445.Google Scholar
Boutsalis, P. 2001. Syngenta quick-test: a rapid whole-plant test for herbicide resistance. Weed Technol 15:257–263.CrossRefGoogle Scholar
Brunoehler, R. 2004. Gauging growth of glyphosate resistance. Corn and Soybean Dig. February 15, 2004.Google Scholar
Cromartie, T. H. and Polge, N. D. 2000. An improved assay for shikimic acid and its use as a monitor for the activity of sulfosate. Proc. Weed Sci. Soc. Am 40:291.Google Scholar
Escorial, M. C., Sixto, H., Gracia-Baudin, J. M., and Chueca, M. C. 2001. A rapid method to determine cereal plant response to glyphosate. Weed Technol 15:697–702.Google Scholar
Feng, P. C. C., Tran, M., Chiu, T., Sammons, R. D., Heck, G. R., and Jacob, C. A. 2004. Investigations into glyphosate-resistant horseweed (Conyza canadensis): retention, uptake, translocation, and metabolism. Weed Sci 52:498–505.Google Scholar
Gerwick, B. C., Mireles, L. C., and Eilers, R. J. 1993. Rapid diagnosis of ALS/AHAS-resistant weeds. Weed Technol 7:519–524.CrossRefGoogle Scholar
Harring, T., Streibig, J. C., and Husted, S. 1998. Accumulation of shikimic acid: a technique for screening glyphosate efficacy. J. Agric. Food Chem 46:4406–4412.CrossRefGoogle Scholar
Heap, I. 2004. The international survey of herbicide resistant weeds. www.weedscience.com. Accessed October 13, 2004.Google Scholar
Hensley, J. R. 1981. A method for identification of triazine resistant and susceptible biotypes of several weeds. Weed Sci 21:70–73.Google Scholar
Lydon, J. and Duke, S. O. 1988. Glyphosate induction of elevated levels of hydroxybenzoic acids in higher plants. J. Agric. Food Chem 36:813–816.Google Scholar
Perez, A. and Kogan, M. 2003. Glyphosate-resistant Lolium multiflorum in Chilean orchards. Weed Res 43:12–19.Google Scholar
Pline, W. A., Wilcut, J. W., Duke, S. O., Edmisten, K. L., and Wells, R. 2002. Tolerance and accumulation of shikimic acid in response to glyphosate applications in glyphosate-resistant and nonglyphosate-resistant cotton (Gossypium hirsutum L). J. Agric. Food Chem 50:506–512.Google Scholar
Reddy, K. N. and Koger, C. H. 2005. Herbicide resistant crops and weed management. in Singh, H. P., ed. Handbook of Sustainable Weed Management. Binghamton, NY: Haworth.Google Scholar
Schmid, J. and Amrhein, N. 1999. The shikimate pathway. Pages 147–170 in Singh, B. J. ed. Plant Amino Acids: Biochemistry and Biotechnology. New York: Marcel Dekker.Google Scholar
Shaner, D. 2000. The impact of glyphosate-tolerant crops on the use of other herbicides and on resistance management. Pestic. Sci 56:320–326.Google Scholar
Shim, S. I., Lee, B. M., Ryu, E. I., and King, B. H. 2003. Response of leaf acetolactate synthase from different leaf positions and seedling ages to sulfonylurea herbicide. Pest. Biochem. Physiol 75:39–46.Google Scholar
Simarmata, M., Kaufmann, J. E., and Penner, D. 2003. Potential basis of glyphosate resistance in California rigid ryegrass (Lolium rigidum). Weed Sci 51:678–682.CrossRefGoogle Scholar
Singh, B. J. 1999. Biosynthesis of valine, leucine and isoleucine. Pages 227–248 in Singh, B. J. ed. Plant Amino Acids: Biochemistry and Biotechnology. New York: Marcel Dekker.Google Scholar
Singh, B. J. and Shaner, D. L. 1998. Rapid determination of glyphosate injury to plants and identification of glyphosate-resistant plants. Weed Technol 12:527–530.Google Scholar
Steinrucken, H. C. and Amrhein, N. 1980. The herbicide glyphosate is a potent inhibitor of 5-enolpyruvyl-shikimic acid-3-phosphate. Biochem. Biophys. Res. Commun 94:1207–1212.Google Scholar
Tal, A., Kotoula-Syka, E., and Rubin, B. 2000. Seed-bioassay to direct grass weeds resistant to acetyl coenzyme A carboxylase inhibiting herbicides. Crop Prot 19:467–472.Google Scholar
Tokhver, A. K. and Pal'm, E. V. 1986. Light-dependence of the inhibiting action of glyphosate on the shikimate pathway in cotyledon leaves of buckwheat seedlings. Soviet Plant Physiol 33:748–753.Google Scholar
Uchino, A., Itoh, K., Wang, G. X., and Watanabe, H. 1999. Light requirement in rapid diagnosis of sulfonylurea-resistant weeds of Lindernia spp. (Scrophulariaceae). Weed Technol 13:680–684.CrossRefGoogle Scholar
USDA-NASS. 2004. Acreage. http://usda.mannlib.cornell.edu/reports/nassr/field/pcp-bba/acrg0604.pdf. Accessed November 2, 2004.Google Scholar
VanGessel, M. J. 2001. Glyphosate-resistant horseweed from Delaware. Weed Sci 49:703–705.Google Scholar
Westwood, J. H. and Weller, S. C. 1997. Cellular mechanisms influence differential glyphosate sensitivity in field bindweed (Convolvulus arvensis) biotypes. Weed Sci 45:2–11.Google Scholar