Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-24T15:50:43.277Z Has data issue: false hasContentIssue false
  • English
  • Français

A quick, simple, and accurate method of screening herbicide activity using green algae cell suspension cultures

Published online by Cambridge University Press:  20 January 2017

Jianyi Ma ,
Ligen Xu  and
Shufeng Wang
Ligen Xu
Affiliation:
College of Life Sciences, Zhejiang University, Hangzhou 310012, People's Republic of China
Shufeng Wang
Affiliation:
College of Life Sciences, Zhejiang University, Hangzhou 310012, People's Republic of China
Get access Rights & Permissions [Opens in a new window]

Abstract

Green algal cell suspensions are used in agrochemical research for screening herbicides. This paper reports on the inhibitory effects of 39 herbicides from 19 different chemical classes with nine different modes of action on green algae cultured in liquid media. The results show that herbicide screening using Chlorella as a model organism is simple, quick, and sensitive. In vitro systems can be a second screening net to ensure that potential new structures are not missed. A set of biotests using cell suspension cultures of Chlorella are suggested as a herbicide prescreen to eliminate inactive compounds before testing on higher plants in the greenhouse. The method has been shown to be suitable for the requirements of microscreen and high-throughput screening (HTS) in vitro. A large-scale microscreen has been devised, which cultures algal cells using self-made Erlenmeyer flasks containing 5 ml of liquid medium. They are particularly useful for HTS compounds with dissimilar modes of action.

Keywords

Green algaeChlorella pyrenoidosa chickHerbicidal activityhigh-throughput screeningcell suspension culturegreen algae
Type
Research Article
Information
Weed Science , Volume 50 , Issue 5 , October 2002 , pp. 555 - 559
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

Frank, T. and Horst, L. G. 1996. Effects of cytochrome P450-interacting plant growth retardants, fungicides and related compounds on cell development and phase-I biotransformation capacity of unicellular photoautotrophic green algae. Pestic. Sci. 47:337–346.Google Scholar
Hess, F. D. 1980. A Chlamydomonas algal bioassay for detecting growth inhibitor herbicides. Weed Sci. 28:515–520.CrossRefGoogle Scholar
Jay, A. E. 1996. Toxic effect of organic solvents on the growth of Chlorella vulgaris and Selenastrum capricornutum . Bull. Environ. Contam. Toxicol. 57:191–198.Google Scholar
Klaus, G., Rainer, B., and Gunter, R. 1992. Heterotrophic plant cell suspension cultures for monitoring biological activity in agrochemical research: comparison with screens using algae, germinating seeds and whole plants. Pestic. Sci. 35:283–289.Google Scholar
Li, S. H. 1959. Mass culture of unicellular green algae. Acta Hydrobiol. Sin. 4:121–129.Google Scholar
Michel, C., Jochen, S., and Peter, B. 1998. A specific and sensitive assay to quantify the herbicidal activity of chloroacetamides. Pestic. Sci. 52:381–387.Google Scholar
Moreland, D. E. 1999. Biochemical mechanisms of action of herbicides and the impact of biotechnology on the development of herbicides. J. Pestic. Sci. 24:299–307.Google Scholar
O’Neal, S. W. and Lembi, C. 1983. Effect of simazine on photosynthesis and growth of filamentous algae. Weed Sci. 31:899–903.CrossRefGoogle Scholar
Retzinger, F. J. and Smith, C. M. 1997. Classification of herbicides by site of action for weed resistance management strategies. Weed Technol. 11:384–396.Google Scholar
Stratton, G. W. 1985. The influence of solvent type on solvent-pesticide interactions in bioassays. Arch. Environ. Contam. Toxicol. 14:651–658.CrossRefGoogle Scholar
Stratton, G. W. and Smith, T. M. 1988. Interaction of organic solvents with the green algae Chlorella pyrenoidesa . Bull. Environ. Contam. Toxicol. 40:736–742.CrossRefGoogle Scholar
Stuart, M. R. and Elliott, A. C. 1998. High-throughput screening as a tool for agrochemical discovery: automated synthesis, compound input, assay design and process management. Pestic. Sci. 54:327–337.Google Scholar
Su, S. Q. 1998. Classification of herbicides by target of action and herbicides use. China Pestic. 37:1–7.Google Scholar
The Chinese National Environmental Protection Agency. 1990. Guidelines for Testing of Chemicals. Beijing: The Chinese Chemical Industry Press.Google Scholar
Thomas, V. M., Buckley, L. J., Sullivan, J. D., and Ikawa, M. 1973. Effect of herbicides on the growth of Chlorella and Bacillus using the paper disc method. Weed Sci. 21:449–451.CrossRefGoogle Scholar
Zweig, G., Hitt, J. D., and McMahon, R. 1968. Effect of certain quinines, diquat, and diuron on Chlorella pyrenoidosa chick, (Emerson strain). Weed Sci. 16:69–73.Google Scholar