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Plastidic ACCase Ile-1781-Leu is present in pinoxaden-resistant southern crabgrass (Digitaria ciliaris)

Published online by Cambridge University Press:  09 October 2019

Suma Basak Open the ORCID record for Suma Basak [Opens in a new window] ,
J. Scott McElroy ,
Austin M. Brown ,
Clebson G. Gonçalves Open the ORCID record for Clebson G. Gonçalves [Opens in a new window] ,
Jinesh D. Patel  and
Patrick E. McCullough
Suma Basak*
Affiliation:
Graduate Research Assistant, Department of Crop, Soil, and Environmental Sciences, Auburn University, Auburn, AL, USA
J. Scott McElroy
Affiliation:
Professor, Department of Crop, Soil, and Environmental Sciences, Auburn University, Auburn, AL, USA
Austin M. Brown
Affiliation:
Graduate Research Assistant, Department of Crop, Soil, and Environmental Sciences, Auburn University, Auburn, AL, USA
Clebson G. Gonçalves
Affiliation:
Graduate Research Assistant, Department of Crop, Soil, and Environmental Sciences, Auburn University, Auburn, AL, USA
Jinesh D. Patel
Affiliation:
Research Associate, Department of Crop, Soil, and Environmental Sciences, Auburn University, Auburn, AL, USA
Patrick E. McCullough
Affiliation:
Associate Professor, Department of Crop and Soil Sciences, University of Georgia, Griffin, GA, USA
*
Author for correspondence: Suma Basak, Department of Crop, Soil, and Environmental Sciences, 201 Funchess Hall, Auburn University, Auburn, AL 36849. Email: [email protected]
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Abstract

Southern crabgrass [Digitaria ciliaris (Retz.) Koeler] is an annual grass weed that commonly infests turfgrass, roadsides, wastelands, and cropping systems throughout the southeastern United States. Two biotypes of D. ciliaris (R1 and R2) with known resistance to cyclohexanediones (DIMs) and aryloxyphenoxypropionates (FOPs) previously collected from sod production fields in Georgia were compared with a separate susceptible biotype (S) collected from Alabama for the responses to pinoxaden and to explore the possible mechanisms of resistance. Increasing rates of pinoxaden (0.1 to 23.5 kg ha−1) were evaluated for control of R1, R2, and S. The resistant biotypes, R1 and R2, were resistant to pinoxaden relative to S. The S biotype was completely controlled at rates of 11.8 and 23.5 kg ha−1, resulting in no aboveground biomass at 14 d after treatment. Pinoxaden rates at which tiller length and aboveground biomass would be reduced 50% (I50) and 90% (I90) for R1, R2, and S ranged from 7.2 to 13.2 kg ha−1, 6.9 to 8.6 kg ha−1, and 0.7 to 2.1 kg ha−1, respectively, for tiller length, and 7.7 to 10.2 kg ha−1, 7.2 to 7.9 kg ha−1, and 1.6 to 2.3 kg ha−1, respectively, for aboveground biomass. Prior selection pressure from DIM and FOP herbicides could result in the evolution of D. ciliaris cross-resistance to pinoxaden herbicides. Amplification of the carboxyl-transferase domain of the plastidic ACCase by standard PCR identified a point mutation resulting in an Ile-1781-Leu amino acid substitution only for the resistant biotype, R1. Further cloning of PCR product surrounding the 1781 region yielded two distinct ACCase gene sequences, Ile-1781 and Leu-1781. The amino acid substitution, Ile-1781-Leu in both resistant biotypes (R1 and R2), however, was revealed by next-generation sequencing of RNA using Illumina platform. A point mutation in the Ile-1781 codon leading to herbicide insensitivity in the ACCase enzyme has been previously reported in other grass species. Our research confirms that the Ile-1781-Leu substitution is present in pinoxaden-resistant D. ciliaris.

Keywords

ACCase inhibitorcloningherbicide resistancenext-generation sequencingpinoxaden resistancepolymerase chain reactionpostemergencetarget site mutationturfgrass
Type
Research Article
Information
Weed Science , Volume 68 , Issue 1 , January 2020 , pp. 41 - 50
Copyright
© Weed Science Society of America, 2019

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Footnotes

Associate Editor: Dean Riechers, University of Illinois

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