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Physiological basis for differential tolerance of tomato and pepper to rimsulfuron and halosulfuron: site of action study

Published online by Cambridge University Press:  20 January 2017

Richard S. Buker III ,
Bala Rathinasabapathi ,
William M. Stall ,
Greg MacDonald  and
Steven M. Olson
Bala Rathinasabapathi
Affiliation:
Horticultural Sciences Department, University of Florida, Gainesville, FL 32611
William M. Stall
Affiliation:
Horticultural Sciences Department, University of Florida, Gainesville, FL 32611
Greg MacDonald
Affiliation:
Agronomy Department, University of Florida, Gainesville, FL 32611
Steven M. Olson
Affiliation:
Horticultural Sciences Department, University of Florida, North Florida Research and Education Center, Quincy, FL 32351
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Abstract

Tomato and pepper differ in their whole-plant tolerance to sulfonylurea (SU) herbicides rimsulfuron and halosulfuron despite both being members of the Solanaceae family. This study examined whether tomato's tolerance to SU herbicides rimsulfuron and halosulfuron was due to insensitivity of the target enzyme acetolactate synthase (ALS). Rimsulfuron and halosulfuron inhibited ALS from both tomato and pepper leaves. Enzyme inhibition and kinetic analyses showed that extractable ALS from tomato was more sensitive to rimsulfuron and halosulfuron than ALS from pepper. ALS from both species were inhibited with a mixed inhibition pattern. Thus, results indicate that enzyme insensitivity is not the reason why tomato is more tolerant than pepper to these herbicides. Tomato tolerance to rimsulfuron at the whole-plant level was reduced in the presence of terbufos, a known inhibitor of cytochrome P450 enzymes. Rimsulfuron applied at 0.018 and 0.035 kg ha−1 with 1.1 kg ha−1 of terbufos reduced tomato shoot weight 69 and 66%, respectively, compared with a 4 and 29% reduction when rimsulfuron was applied alone. The reduction of tomato tolerance to rimsulfuron by terbufos suggests that the sensitivity differences between these species may reflect their differences in SU herbicide metabolism.

Keywords

Halosulfuronrimsulfuronpepper, Capsicum annuum L. ‘X3R-Camelot’tomato, Lycopersicon esculentum Mill. ‘FL 47’Enzyme sensitivityenzyme inhibitionherbicide metabolismherbicide–insecticide interaction
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 52 , Issue 2 , April 2004 , pp. 201 - 205
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Ackley, J. A., Hatzios, K. K., and Wilson, H. P. 1999. Absorption, translocation, and metabolism of rimsulfuron in black nightshade (Solanum nigrum), eastern black nightshade (Solanum ptycanthum), and hairy nightshade (Solanum sarrachoides). Weed Technol 13:151–156.Google Scholar
Boutsalis, P. and Powles, S. B. 1995. Inheritance and mechanism of resistance to herbicides inhibiting acetolactate synthase in Sonchus oleraceus L. Theor. Appl. Genet 91:242–247.CrossRefGoogle ScholarPubMed
Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem 72:248–254.Google Scholar
Buker, R. S. III. 2002. Differential tolerance of tomato (Lycpersicum esculentum Mill.) and pepper (Capsicum annuum L.) to rimsulfuron and halosulfuron herbicides. Ph.D. dissertation. University of Florida, Gainesville, FL. Pp. 44–52.Google Scholar
Chaleff, R. S. and Mauvais, C. J. 1984. Acetolactate synthase is the site of action of two sulfonylurea herbicides in higher plants. Science 224:1443–1445.CrossRefGoogle ScholarPubMed
Cornish-Bowden, A. 1995. Analysis of Enzyme Kinetic Data. Oxford, U.K.: Oxford University Press. Pp. 1–200.Google Scholar
Devine, M. D., Duke, S. O., and Fedtke, C. 1993. Physiology of Herbicide Action. Englewood Cliffs, NJ: Prentice Hall.Google Scholar
Durner, J. and Boger, P. 1988. Acetolactate synthase from barley (Hordeum vulgare L.): purification and partial characterization. Z. Naturforsch 43:850–856.Google Scholar
Eberlein, C. V., Guttieri, M. J., Smith, C. A., Thill, D. C., and Baerg, R. J. 1997. Altered acetolactate synthase activity in ALS-inhibitor resistant prickly lettuce (Lactuca serriola). Weed Sci 45:212–217.Google Scholar
Fonné-Pfister, R., Gaudin, J., Kreuz, K., Ramsteiner, K., and Ebert, E. 1990. Hydroxylation of primisulfuron by an inducible cytochrome P450-dependent monooxygenase system from maize. Pestic. Biochem. Physiol 37:165–173.Google Scholar
Frear, D. S., Swanson, H. R., and Thalacker, F. W. 1991. Induced microsomal oxidation of dicloflop, triasulfuron, chlorsulfuron, and linuron in wheat. Pestic. Biochem. Physiol 41:274–287.Google Scholar
Gee, S. K. and Hay, J. V. 1994. Recent developments in the chemistry of sulfonylurea herbicides. Pages 15–16 in Stetter, J. and Ebing, W. eds. Chemistry of Plant Protection—Herbicides Inhibiting Branched-Chain Amino Acid Biosynthesis. Berlin: Springer-Verlag.Google Scholar
Green, J. M. and Green, J. H. 1993. Surfactant structure and concentration strongly affect rimsulfuron activity. Weed Technol 7:633–640.Google Scholar
Henderson, P. J. 1995. Statistical analysis of enzyme kinetic data. Pages 277–316 in Eisenthal, R. and Danson, M. J. eds. Enzyme Assays, A Practical Approach. Oxford, U.K.: IRL.Google Scholar
Holshouser, D. L., Chandler, J. M., and Smith, H. R. 1991. The influence of terbufos on the response of five corn (Zea mays) hybrids to CGA-136872. Weed Technol 5:165–168.CrossRefGoogle Scholar
Koeppe, M., Hirata, C. M., Brown, H. M., Kenyon, W. H., O'Keefe, D. P., Lau, S. C., Zimmerman, W. T., and Green, J. M. 2000. Basis of selectivity of rimsulfuron in maize. Pestic. Biochem. Physiol 66:170–181.CrossRefGoogle Scholar
Morton, C. A., Harvey, R. G., Kells, J. J., Lueschen, W. E., and Fritz, V. A. 1991. Effect of DPX-V9360 and terbufos on field and sweet corn (Zea mays) under three environments. Weed Technol 5:130–136.Google Scholar
Rathinasabapathi, B., Fouad, W. M., and Sigua, C. A. 2001. β-Alanine betaine synthesis in the Plumbaginaceae. Purification and characterization of a trifunctional, S-adenosyl-L-methionine-dependent N-methyltransferase from Limonium latifolium leaves. Plant Physiol 126:1241–1249.Google Scholar
Rathinasabapathi, B. and King, J. 1991. Herbicide resistance in Datura innoxia: kinetic characterization of acetolactate synthase from wild-type and sulfonylurea-resistant cell variants. Plant Physiol 96:255–261.CrossRefGoogle ScholarPubMed
Robinson, D. K., Monks, D. W., and Burton, J. D. 1996. Safening influence of LAB145 138 on nicosulfuron, terbufos, and bentazon interactions in sweet corn (Zea mays). Weed Sci 44:339–344.CrossRefGoogle Scholar
Saari, L. L., Cotterman, J. C., Smith, W. F., and Primiani, M. M. 1992. Sulfonylurea herbicide resistance in common chickweed, perennial ryegrass, and Russian thistle. Pestic. Biochem. Physiol 42:110–118.CrossRefGoogle Scholar
Scarponi, L., Esposito, A., and Tomassini, C. 2001. Factors of tolerance to rimsulfuron in four pepper (Capsicum annuum) lines. Agronomie 21:419–425.Google Scholar
Schroeder, J. 1998. Cucumber (Cucumis sativus) response to selected foliar- and soil-applied sulfonylurea herbicides. Weed Technol 12:595–601.CrossRefGoogle Scholar
Siminszky, B., Corbin, F. T., and Sheldon, Y. 1995. Nicosulfuron resistance and metabolism in terbufos- and naphthalic anhydride-treated corn. Weed Sci 43:163–168.Google Scholar
Simpson, D. W., Diehl, K. E., and Stoller, E. W. 1994. 2, 4-D safening of nicosulfuron and terbufos interaction in corn (Zea mays). Weed Technol 8:547–552.Google Scholar
Singh, B. K., Stidham, M. A., and Shaner, D. L. 1988. Separation and characterization of two forms of acetohydroxy acid synthase from black Mexican sweet corn cells. J. Chromatogr 444:251–261.CrossRefGoogle Scholar
Stall, W. M. 1999. Tolerance of bell pepper to rimsulfuron and halosulfuron. Proc. South. Weed Sci. Soc 52:83.Google Scholar
Stall, W. M. and Bewick, T. A. 1992. Sweet corn cultivars respond differentially to the herbicide nicosulfuron. Hortscience 27:131–133.Google Scholar
Tipton, K. F. 1995. Principles of enzyme assay and kinetic studies. Pages 1–53 in Eisenthal, R. and Dawson, M. J. eds. Enzyme Assays. Oxford, U.K.: Oxford Press.Google Scholar
Westerfield, W. W. 1945. A colorimetric determination of blood acetoin. J. Biol. Chem 161:495–502.Google Scholar
Wilson, H. P., Monks, D. W., Hines, T. E., and Mills, R. J. 2001. Responses of potato (Solanum tuberosum), tomato (Lycopersicon esculentum) and several weeds to ASC-67040 herbicide. Weed Technol 15:271–276.Google Scholar
Zubay, G. L. 1998. Biochemistry. 4th ed. Dubuque, IA: W. C. Brown. pp. 171, 581.Google Scholar