Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-30T19:57:15.484Z Has data issue: false hasContentIssue false
  • English
  • Français

Lack of Cross-Resistance of Paraquat-Resistant Hairy Fleabane (Conyza bonariensis) to Other Toxic Oxygen Generators Indicates Enzymatic Protection is Not the Resistance Mechanism

Published online by Cambridge University Press:  12 June 2017

Kevin C. Vaughn ,
Martin A. Vaughan  and
Patrick Camilleri
Kevin C. Vaughn
Affiliation:
Plant Physiol's., USDA, ARS, South. Weed Sci. Lab., Stoneville, MS 38776 Res. Chem., Shell Res., Ltd., Sittingbourne Res. Ctr., Sittingbourne, Kent ME9 8AG, United Kingdom
Martin A. Vaughan
Affiliation:
Plant Physiol's., USDA, ARS, South. Weed Sci. Lab., Stoneville, MS 38776 Res. Chem., Shell Res., Ltd., Sittingbourne Res. Ctr., Sittingbourne, Kent ME9 8AG, United Kingdom
Patrick Camilleri
Affiliation:
Plant Physiol's., USDA, ARS, South. Weed Sci. Lab., Stoneville, MS 38776 Res. Chem., Shell Res., Ltd., Sittingbourne Res. Ctr., Sittingbourne, Kent ME9 8AG, United Kingdom
Get access Rights & Permissions [Opens in a new window]

Abstract

Cross-resistance of the paraquat-resistant (R) hairy fleabane to other compounds that accept electrons from photosystem I (PSI) or produce toxic oxygen species was determined by chlorophyll loss, electron microscopy, and chlorophyll fluorescence suppression. Although the R bioype is approximately 100 x more resistant to paraquat than the susceptible (S) biotype based upon the assays for tissue damage, little or no cross-resistance was observed to a number of other PSI electron acceptors, including the bipyridilium herbicide morfamquat. A low level of resistance (approximately 10-fold) was noted to diquat and the singlet oxygen generator rose bengal. As measured by chlorophyll fluorescence suppression, the R biotype was about 100-fold resistant to paraquat, but only 10-fold resistant to diquat, and exhibited no resistance to morfamquat. Because differences observed with this protocol are direct measures of the ability of the herbicide to reach the active site and the results correlate with the level of resistance observed by chlorophyll bleaching or electron microscopy, these data suggest that compartmentalization is the major factor in paraquat resistance in hairy fleabane.

Keywords

Paraquat, 1,1′-dimethyl-4,4′-bipyridinum ionhairy fleabane, Conyza bonariensis (L.) Cron. # ERIBOmorfamquat, 1,1′ - di - (3,5 - dimethylmorpholinocarbonylmethyl) - 4,4′ - bipyridyldiyliumdiquat, 6,7-dihydrodipyrido[1,2-α:2′,1′-c] pyrazinediium ionDiquatmorfamquatheteropentalene herbicides
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 37 , Issue 1 , January 1989 , pp. 5 - 11
Copyright
Copyright © 1989 by the Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Arntzen, C. J., Fister, K. P., and Steinback, K. E. 1982. The mechanism of chloroplast triazine resistance: Alterations in the site of action. Pages 185–214 in LeBaron, H. M. and Gressel, J., eds. Herbicide Resistance in Plants. John Wiley and Sons, New York.Google Scholar
2. Bishop, T., Powles, S. B., and Cornic, G. 1987. Mechanism of paraquat resistance in Hordeum glaucum: 2. Studies of paraquat uptake and translocation. Aust. J. Plant Physiol. 14:539–547.Google Scholar
3. Brian, R. C. 1972. Observations on the physiological basis for the selectivity of morfamquat to graminaceous plants. Pestic. Sci. 3:409–414.CrossRefGoogle Scholar
4. Camilleri, P., Clark, M. T., Gilmore, I. J., and Cole-Hamilton, D. 1985. The synthesis and electrochemical properties of some novel photosynthesis electron acceptors. J. Chem. Soc. Perkin Trans. II. 1985:833–836.CrossRefGoogle Scholar
5. Camilleri, P., Bowyer, J. R., and McNeil, P. H. 1987. The effects of photosystem I electron acceptors on leaf discs. Z. Naturforsch. 42C:829–833.CrossRefGoogle Scholar
6. Elstner, E. F., Fischer, H. P., Osswald, W., and Kwiatkowski, G. 1980. Superoxide- and ethane-formation of subchloroplast particles: catalysis by pyridinium derivatives. Z. Naturforsch. 35C:770–775.CrossRefGoogle Scholar
7. Faulkner, J. S. 1982. Breeding herbicide-tolerant crop cultivars by conventional methods. Pages 235–256 in LeBaron, H. M. and Gressel, J., eds. Herbicide Resistance in Plants. John Wiley and Sons, New York.Google Scholar
8. Fuerst, E. P., Nakatani, H. Y., Dodge, A. D., Penner, D., and Arntzen, C. J. 1985. Paraquat resistance in Conyza . Plant Physiol. 77:984–989.CrossRefGoogle ScholarPubMed
9. Fuerst, E. P., Arntzen, C. J., Pfister, K., and Penner, D. 1986. Herbicide cross-resistance in triazine-resistant biotypes of four species. Weed Sci. 34:344–353.CrossRefGoogle Scholar
10. Harvey, B.M.R. and Fraser, T. W. 1980. Paraquat tolerant and susceptible perennial ryegrasses: Effects of paraquat treatment on carbon dioxide uptake and ultrastructure of photosynthetic cells. Plant Cell Environ. 3:107–117.CrossRefGoogle Scholar
11. Itoh, K. and Miyahara, M. 1984. Inheritance of paraquat resistance in Erigeron philadelphicus . Weed Res. (Japan) 29:47–52.Google Scholar
12. Jensen, K.I.N. 1982. The roles of uptake, translocation, and metabolism in the differential intraspecific responses to herbicides. Pages 133–162 in LeBaron, H. M. and Gressel, J., eds. Herbicide Resistance in Plants. John Wiley and Sons, New York.Google Scholar
13. Knox, J. P. and Dodge, A. D. 1984. Photodynamic damage to plant leaf tissue by rose bengal. Plant Sci. Lett. 37:307.CrossRefGoogle Scholar
14. Lax, A. R. and Vaughn, K. C. 1986. Lack of correlation between effects of tentoxin on chloroplast coupling factor and chloroplast ultrastructure. Physiol. Plant. 66:304–391.CrossRefGoogle Scholar
15. Shaaltiel, Y. and Gressel, J. 1986. Multienzyme oxygen radical detoxification system correlated with paraquat resistance in Conyza bonariensis . Pestic. Biochem. physiol. 26:22–28.CrossRefGoogle Scholar
16. Tanaka, Y., Chisaka, H., and Saka, H. 1986. Movement of paraquat in resistant and susceptible biotypes of Erigeron philadelphicus and E. canadensis . Physiol. Plant. 66:605–608.CrossRefGoogle Scholar
17. Vaughn, K. C. and Duke, S. O. 1984. Ultrastructural alterations to chloroplasts in triazine-resistant weed biotypes. Physiol. Plant. 62:510–520.CrossRefGoogle Scholar
18. Vaughn, K. C. and Fuerst, E. P. 1985. Structural and physiological studies of paraquat-resistant Conyza . Pestic. Biochem. Physiol. 24:86–94.CrossRefGoogle Scholar
19. Vaughn, K. C., Marks, D., and Weeks, D. P. 1987. A dinitroaniline-resistant mutant of Eleusine indica exhibits cross-resistance and supersensitivity to antimicrotubule herbicides and drugs. Plant Physiol. 83:956–964.CrossRefGoogle ScholarPubMed
20. Youngman, R. J. and Dodge, A. D. 1981. On the mechanism of paraquat resistance to Conyza sp. Pages 537–544 in Akoyunoglou, G., ed. Proc. Int. Congr. Photosynth. Vol. 6. Balaban Press, Tel Aviv, Israel.Google Scholar