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Growth Characterization of Kochia (Kochia scoparia) with Substitutions at Pro197 or Trp574 Conferring Resistance to Acetolactate Synthase–Inhibiting Herbicides

Published online by Cambridge University Press:  20 January 2017

Anne Légère ,
F. Craig Stevenson ,
Hugh J. Beckie ,
Suzanne I. Warwick ,
Eric N. Johnson ,
Brett Hrynewich  and
Chris Lozinski
Anne Légère*
Affiliation:
Agriculture and Agri-Food Canada (AAFC), Saskatoon Research Centre, 107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada
F. Craig Stevenson
Affiliation:
142 Rogers Road, Saskatoon, Saskatchewan, S7N 3T6, Canada
Hugh J. Beckie
Affiliation:
Agriculture and Agri-Food Canada (AAFC), Saskatoon Research Centre, 107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada
Suzanne I. Warwick
Affiliation:
AAFC, Eastern Cereal and Oilseed Research Centre, K. W. Neatby Building, Central Experimental Farm, Ottawa, Ontario K1A 0C6, Canada
Eric N. Johnson
Affiliation:
AAFC, Scott Research Farm, P.O. Box 10, Scott, Saskatchewan, S0K 4A0, Canada
Brett Hrynewich
Affiliation:
Agriculture and Agri-Food Canada (AAFC), Saskatoon Research Centre, 107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada
Chris Lozinski
Affiliation:
Agriculture and Agri-Food Canada (AAFC), Saskatoon Research Centre, 107 Science Place, Saskatoon, Saskatchewan S7N 0X2, Canada
*
Corresponding author's E-mail: [email protected]
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Abstract

Over 90% of Canadian kochia populations are resistant to acetolactate synthase (ALS)– inhibiting herbicides. We questioned whether the target site–based resistance could affect plant growth and competitiveness. Homozygous F2 herbicide-resistant (HR) kochia plants with an amino acid substitution at Trp574 (sources: Alberta [AB], Saskatchewan [SK], and Manitoba [MB]), or Pro197 (MB, AB with two populations) were grown in replacement series with homozygous F2 herbicide-susceptible (HS) plants from the corresponding heterogeneous population (total: six populations). In pure stands, growth of HR plants from AB and SK was similar to that of HS plants, regardless of mutation; conversely, MB2-HR plants (Trp574Leu) developed more slowly and were taller than MB2-HS plants. Final dry weight of HR plants in pure stands was similar across all six populations, whereas that for HS plants in pure stands and HR–HS plants in mixed stands (50–50%) varied with population. Results for AB and SK populations suggest little impact of either ALS mutation on kochia growth, whereas those for MB lines would suggest an unidentified factor (or factors) affecting the HS, HR, or both biotypes. The variable response within and between lines, and across HS biotypes highlights the importance of including populations of various origins and multiple susceptible controls in HR biotype studies.

Keywords

Kochia, Kochia scoparia (L.) Schrad. KCHSCAcetohydroxyacid synthaseAHASALScost of herbicide resistancePro197 mutationTrp574 mutation
Type
Weed Biology and Ecology
Information
Weed Science , Volume 61 , Issue 2 , June 2013 , pp. 267 - 276
Copyright
Copyright © Weed Science Society of America 

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