Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-18T10:19:25.458Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of Sc-0224 and Glyphosate On Free Amino Acids, Soluble Protein, and Protein Synthesis in Inflated Duckweed (Lemna Gibba)

Published online by Cambridge University Press:  12 June 2017

William E. Cooley  and
Chester L. Foy
William E. Cooley
Affiliation:
Dep. Plant Pathol., Physiol., Weed Sci., Virginia Polytech. Inst. State Univ., Blacksburg, VA 24061
Chester L. Foy
Affiliation:
Dep. Plant Pathol., Physiol., Weed Sci., Virginia Polytech. Inst. State Univ., Blacksburg, VA 24061
Get access Rights & Permissions [Opens in a new window]

Abstract

Effects of the herbicides SC-0224 and glyphosate on the pool levels of free amino acids, soluble protein, and protein synthesis in inflated duckweed were compared. SC-0224 caused larger increases than glyphosate in the pool levels of amino acids; the increases caused by SC-0224 were similar, however, to those caused by trimethylsulfonium iodide (TMS-I). Expressed on a per gram fresh weight basis, none of the treatments changed soluble protein or the incorporation of 14C-leucine into soluble protein. On a per flask basis (allowing for decreased growth in treated flasks), both herbicides and TMS-I decreased soluble protein and 14C-leucine incorporation. Decreases in soluble protein and 14C-leucine incorporation were equal for SC-0224 and TMS-I but larger than for glyphosate. These data indicate that differences in the phytotoxicity of SC-0224 and glyphosate may be due to the action of the trimethylsulfonium portion of the SC-0224 structure.

Keywords

Glyphosate, N-(phosphonomethyl)glycineSC-0224, trimethylsulfonium carboxymethyl-aminomethylphosphonateTMS-I, trimethylsulfonium iodideinflated duckweed, Lemna gibba L. # LEMGITrimethylsulfonium iodideLEMGI
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 40 , Issue 3 , September 1992 , pp. 345 - 350
Copyright
Copyright © 1992 by the Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Amrhein, N., Deus, B., Gehrke, P., and Steinrucken, H. C. 1980. The site of the inhibition of shikimate pathway by glyphosate. II. Interference of glyphosate with chorismate formation in vivo and in vitro . Plant Physiol. 66:830–834.CrossRefGoogle ScholarPubMed
2. Amrhein, N., Johanning, D., Schab, H., and Schultz, A. 1983. Biochemical basis for glyphosate-tolerance in a bacterium and a plant tissue culture. FEBS Lett. 157:191–196.Google Scholar
3. Becerril, J. M., Duke, S. O., and Lydon, J. 1989. Glyphosate effects on shikimate pathway products in leaves and flowers of velvetleaf. Phytochemistry 28:695–699.Google Scholar
4. Beevers, L. 1976. Nitrogen Metabolism in Plants. William Clowes & Sons, London.Google Scholar
5. Bellinder, R. R. and Wilson, H. P. 1983. Comparison of several nonselective herbicides in reduced tillage systems. Proc. Northeast. Weed Sci. Soc. 37:20–26.Google Scholar
6. Bellinder, R. R., Hatzios, K. K., and Wilson, H. P. 1985. Mode of action investigations with the herbicides HOE-39866 and SC-0224. Weed Sci. 33:779–785.Google Scholar
7. Berlin, J. and Widholm, J. M. 1978. Metabolism of phenylalanine and tyrosine in tobacco cell lines resistant and sensitive to pfluorophenylalanine. Phytochemistry 17:65–68.CrossRefGoogle Scholar
8. Bidwell, R.G.S., Barr, R. A., and Steward, F. C. 1964. Protein synthesis and turnover in cultured plant tissue: sources of carbon for synthesis and the fate of the protein breakdown products. Nature 203:367–373.Google Scholar
9. Bradford, M. M. 1976. A rapid and sensitive method for quantifying microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72:248–254.Google Scholar
10. Campbell, W. F., Evans, J. O., and Reed, S. C. 1976. Effects of glyphosate on chloroplast ultrastructure of quackgrass mesophyll cells. Weed Sci. 24:22–25.CrossRefGoogle Scholar
11. Carlson, K. L. and Burnside, O. C. 1985. Comparative phytotoxicity of glyphosate, SC-0224, SC-0545, and HOE-00661, Weed Sci. 32:841–844.Google Scholar
12. Cleland, C. F. and Briggs, W. R. 1967. Flowering responses of the long day plant Lemna gibba G3. Plant Physiol. 42:1553–1561.Google Scholar
13. Cole, D. J., Dodge, A. D., and Caseley, J. C. 1980. Some biochemical effects of glyphosate on plant meristems. J. Exp. Bot. 31:1665–1674.Google Scholar
14. Comai, L., Sen, C., and Stalker, D. M. 1983. An altered aroA gene product confers resistance to the herbicide glyphosate. Science 221:370–371.Google Scholar
15. Cooley, W. E. and Foy, C. L. 1986. Effects of SC-0224 and glyphosate on inflated duckweed (Lemna gibba) growth and EPSP-synthase activity from Klebsiella pneumoniae . Pestic. Biochem. Physiol. 26:365–374.Google Scholar
16. Cooley, W. E. and Foy, C. L. 1989. Effects of SC-0224 on photosynthetic electron transport. Plant Growth Regul. Soc. Am. Quart. 17:31–38.Google Scholar
17. Duke, S. O., Hoagland, R. E., and Elmore, C. D. 1980. Effects of glyphosate on metabolism of phenolic compounds V. L-α-aminooxy-β phenylpropionic acid and glyphosate effects on phenylalanine ammonialyase. Plant Physiol. 65:17–21.CrossRefGoogle Scholar
18. Foley, M. E., Nafziger, E. D., Slife, F. W., and Wax, L. M. 1983. Effect of glyphosate on protein and nucleic acid synthesis and ATP levels in common cocklebur (Xanthium pensylvanicum) root tissue. Weed Sci. 31:76–80.CrossRefGoogle Scholar
19. Foy, C. L. and Cooley, W. E. 1987. Studies on the comparative mode(s) of herbicidal action of SC-0224 and glyphosate. Abstr. XIV Int. Bot. Congr. Abstr. 1-114-4:71.Google Scholar
20. Gianfagna, T. 1975. Studies on the Mode of Action of Glyphosate. M.S. thesis, Virginia Polytech. Inst. and State Univ., Blacksburg, VA. 65 pp.Google Scholar
21. Haderlie, L. C., Widholm, J. M., and Slife, F. W. 1977. Effect of glyphosate on carrot and tobacco cells. Plant Physiol. 60:40–43.Google Scholar
22. Harvey, R. G. and McNevin, G. R. 1982. Wild proso millet control in stubble planted corn. Res. Rep. North Cent. Weed Control Conf. 39:228–229.Google Scholar
23. Hoagland, R. E. 1989. Biochemical interactions of atrazine and glyphosate in soybean (Glycine max) seedlings. Weed Sci. 37:491–497.Google Scholar
24. Hoagland, R. E. and Duke, S. O. 1982. Biochemical effects of glyphosate [N-(phosphonomethyl)glycine]. Pages 175–205 in Moreland, D. E., St. John, J. B., and Hess, F. D., eds. Biochemical Responses Induced by Herbicides. ACS, Washington, DC.Google Scholar
25. Hoagland, R. E., Duke, S. O., and Elmore, C. D. 1978. Effects of glyphosate on metabolism of phenolic compounds. II. Influence on soluble hydroxyphenolic compounds, free amino acids and soluble protein levels in dark-grown maize roots. Plant Sci. Lett. 13:291–299.CrossRefGoogle Scholar
26. Hoagland, R. E., Duke, S. O., and Elmore, C. D. 1979. Effects of glyphosate on metabolism of phenolic compounds. III. Phenylalanine ammonium-lyase activity, free amino acids, soluble protein, and hydroxyphenolic compounds in axes of dark-grown soybeans. Physiol. Plant. 46:357–366.Google Scholar
27. Hollander, H. and Amrhein, N. 1980. The site of the inhibition of the shikimate pathway by glyphosate I. Inhibition by glyphosate of phenylpropanoid synthesis in buckwheat. Plant Physiol. 66:823–829.Google Scholar
28. Hutchinson, S. A. and Banks, P. A. 1985. Effect of bermudagrass clipping on the activity of two herbicides. Proc. South. Weed Sci. Soc. 38:442.Google Scholar
29. Jaworski, E. G. 1972. Mode of action of N-phosphonomethylglycine: Inhibition of aromatic amino acid biosynthesis. J. Agric. Food Chem. 20:1195–1198.Google Scholar
30. Lydon, J. and Duke, S. O. 1989. Pesticide effects on secondary metabolism of higher plants. Pestic. Sci. 25:361–373.Google Scholar
31. Moore, S., Spackman, D. H., and Stein, W. H. 1958. Chromatography of amino acids on sulfonated polystyrene resins, an improved system. Anal. Chem. 30:1185–1190.Google Scholar
32. Nafziger, E. D., Widholm, J. M., Steinrucken, H. C., and Killmer, J. L. 1984. Selection and characterization of a carrot cell line tolerant to glyphosate. Plant Physiol. 76:571–574.CrossRefGoogle ScholarPubMed
33. Nilsson, G. 1977. Effects of glyphosate on the amino acid content in spring wheat plants. Swed. J. Agric. Res. 7:153–157.Google Scholar
34. Oaks, A. and Bidwell, R.G.S. 1970. Compartmentation of intermediary metabolites. Annu. Rev. Plant Physiol. 21:43–66.Google Scholar
35. Smutny, E. J., Soloway, S. B., Dowding, L. C., and Van Overbeek, J. 1963. Method for preventing undesired plant growth. Page 1–6 in U.S. Pat Off., Pat. No. 3,100,265.Google Scholar
36. Steinrucken, H. C. and Amrhein, N. 1980. The herbicide glyphosate is a potent inhibitor of 5-enolpyruvylshikimic acid-3-phosphate synthase. Biochem. Biophys. Res. Commun. 94:1207–1212.Google Scholar
37. Steinrucken, H. C. and Amrhein, N. 1984. 5-Enolpyruvylshikimate-3-phosphate synthase of Klebsiella pneumoniae 2. Inhibition by glyphosate [N-(phosphonomethyl)glycine]. Eur. J. Biochem. 143:351–357.CrossRefGoogle ScholarPubMed
38. Stoddart, J. L. and Thomas, H. 1982. Leaf senescence. Pages 592–636 in Pirson, A. and Zimmerman, M. H., eds. Encyclopedia of Plant Physiology—New Series. Vol. 14A. Springer-Verlag, New York.Google Scholar
39. Taveres, J. and Kende, H. 1970. The effect of benzyladenine on protein metabolism in senescing corn leaves. Phytochemistry 9:1763–1770.Google Scholar
40. Thomas, H. 1978. Enzymes of nitrogen mobilization in detached leaves of Lolium temulentum during senescence. Planta 142:161–169.Google Scholar
41. Trewavas, A. 1972. Control of the protein turnover rates of Lemna minor . Plant Physiol. 49:47–51.Google Scholar
42. Tymonko, J. M. 1978. Studies of the Metabolic Sites of Action of Glyphosate. Ph.D. dissertation, Virginia Polytech. Inst. and State Univ., Blacksburg, VA. 72 pp.Google Scholar
43. Tymonko, J. M. and Foy, C. L. 1978. Inhibition of protein synthesis by glyphosate. Plant Physiol. (Suppl.) 61:41.Google Scholar
44. Wilson, H. P., Hines, T. E., Bellinder, R. R., and Grande, J. A. 1985. Comparison of HOE-39866, SC-0224, paraquat, and glyphosate in no-till corn (Zea mays). Weed Sci. 33:531–536.Google Scholar