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Effects of Herbicides on ATP Levels in Excised Soybean Hypocotyls

Published online by Cambridge University Press:  12 June 2017

R. D. Gruenhagen  and
D. E. Moreland
R. D. Gruenhagen
Affiliation:
Departments of Crop Science and Botany, North Carolina State Univ., Raleigh, North Carolina Union Carbide Corp., Clayton, North Carolina
D. E. Moreland
Affiliation:
Crops Res. Div., Agr. Res. Serv., U. S. Dep. of Agr., Crop Science Dep., North Carolina State Univ., Raleigh, North Carolina
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Abstract

Effects of 22 herbicides on the ATP content of excised soybean [Glycine max (L.) Merr., var. Lee] hypocotyls were determined and compared with interferences reported previously on RNA and protein syntheses. Herbicides that reduced ATP levels and inhibited RNA and protein syntheses were 2-sec-butyl-4,6-dinitrophenol (dinoseb), 4-hydroxy-3,5-diiodobenzonitrile (ioxynil), 3′,4′-dichloropropionanilide (propanil), isopropyl m-chlorocarbanilate (chlorpropham), 2,3,5-trichloro-4-pyridinol (pyriclor), 2-chloro-N-isopropylacetanilide (propachlor), (2,4,5-trichlorophenoxy)acetic acid (2,4,5-T), and (2,3,6-trichlorophenyl)acetic acid (fenac). No herbicide was found which reduced tissue ATP levels, but did not inhibit RNA and protein syntheses. The correlations established between tissue ATP levels and inhibition of RNA and protein syntheses suggested that interference with the production of energy, required to drive the biosynthetic reactions, could be the mechanism through which these herbicides expressed their effects.

Type
Research Article
Information
Weed Science , Volume 19 , Issue 4 , July 1971 , pp. 319 - 323
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

1. Ashton, F. M. 1963. Effect of EPTC on photosynthesis, respiration, and oxidative phosphorylation. Weeds 11:295–297.CrossRefGoogle Scholar
2. Davis, D. E. 1968. Atrazine effects on mitochondrial respiration. Proc. So. Weed Conf. 21:346.Google Scholar
3. Ferrari, T. E. and Moreland, D. E. 1969. Effects of 3,5-dihalogenated-4-hydroxybenzonitriles on the activity of mitochondria from white potato tubers. Plant Physiol. 44:429–434.CrossRefGoogle ScholarPubMed
4. Foy, C. L. and Penner, D. 1965. Effect of inhibitors and herbicides on tricarboxylic acid cycle substrate oxidation by isolated cucumber mitochondria. Weeds 13:226–231.CrossRefGoogle Scholar
5. Gladtke, E. and Liss, E. 1959. Die Beeinflussung der oxydativen Phosphorylierung von Rattenleber-Mitochondrien durch verschiedene Nitrophenole. Biochem. Z. 331:65–70.Google Scholar
6. Hachimori, A., Nosoh, Y., and Sone, N. 1968. Effects of the photosynthetic inhibitors on the respiration of yeast mitochondria. J. Biochem. (Tokyo) 64:119–121.CrossRefGoogle ScholarPubMed
7. Hemker, H. C. 1964. Inhibition of adenosine triphosphatase and respiration of rat-liver mitochondria by dinitrophenols. Biochim. Biophys. Acta 81:1–8.Google Scholar
8. Hofstra, G. and Switzer, C. M. 1968. The phytotoxicity of propanil. Weed Sci. 16:23–28.CrossRefGoogle Scholar
9. Inoue, Y., Ishizuka, K., and Mitsui, S. 1967. Inhibition of respiration of yeast by photosynthesis inhibiting herbicides. Agr. Biol. Chem. 31:422–427.CrossRefGoogle Scholar
10. Kerr, M. W. and Wain, R. L. 1964. The uncoupling of oxidative phosphorylation in pea shoot mitochondria by 3,5-diiodo-4-hydroxybenzonitrile (ioxynil) and related compounds. Ann. Appl. Biol. 54:441–446.CrossRefGoogle Scholar
11. Killion, D. D. and Frans, R. E. 1969. Effect of pyriclor on mitochondrial oxidation. Weed Sci. 17:468–470.CrossRefGoogle Scholar
12. Lehninger, A. L. 1965. Bioenergetics. W. A. Benjamin Inc., New York. 258 p.Google Scholar
13. Lotlikar, P. D., Remmert, L. F., and Freed, V. H. 1968. Effects of 2,4-D and other herbicides on oxidative phosphorylation in mitochondria from cabbage. Weed Sci. 16:161–165.CrossRefGoogle Scholar
14. Moreland, D. E., Malhotra, S. S., Gruenhagen, R. D., and Shokraii, E. H. 1969. Effects of herbicides on RNA and protein syntheses. Weed Sci. 17:556–562.CrossRefGoogle Scholar
15. Mukasa, H., Itoh, M., and Nosoh, Y. 1966. 3-(p-chlorophenyl)-1,1-dimethylurea as a specific inhibitor of yeast respiration. Plant and Cell Physiol. 7:683–687.CrossRefGoogle Scholar
16. Negi, N. S., Funderburk, H. H. Jr., Schultz, D. P., and Davis, D. E. 1968. Effect of trifluralin and nitralin on mitochondrial activities. Weed Sci. 16:83–85.CrossRefGoogle Scholar
17. Phung-Nhu-Hung, S. 1966. Action du 3-amino-1,2,4-triazol sur la phosphorylation oxydative des mitochondries isolées d'hypocotyles de Cucumis sativus L. Compt. Rend. Acad. Sci., Paris 262:860–863.Google Scholar
18. Schneider, W. C. 1957. Determination of nucleic acids in tissues by pentose analysis, p. 680–684. In Colowick, S. P. and Kaplan, N. O. (ed.), Methods in Enzymology. Vol. III. Academic Press, New York.Google Scholar
19. Strehler, B. L. and Totter, J. R. 1952. Firefly luminescence in the study of energy transfer mechanisms. I. Substrate and enzyme determination. Arch. Biochem. Biophys. 40:28–41.CrossRefGoogle Scholar
20. Switzer, C. M. 1957. Effects of herbicides and related chemicals on oxidation and phosphorylation by isolated soybean mitochondria. Plant Physiol. 32:42–44.CrossRefGoogle ScholarPubMed