Hostname: page-component-cd9895bd7-jkksz Total loading time: 0 Render date: 2024-12-24T17:57:07.335Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of Sethoxydim and Haloxyfop on Acetyl-Coenzyme a Carboxylase Activity in Festuca Species

Published online by Cambridge University Press:  12 June 2017

David E. Stoltenberg ,
John W. Gronwald ,
Donald L. Wyse ,
James D. Burton ,
David A. Somers  and
Burle G. Gengenbach
David E. Stoltenberg
Affiliation:
Dep. Agron. and Plant Genetics, Univ. Minnesota, St. Paul, MN 55108
John W. Gronwald
Affiliation:
Dep. Agron. and Plant Genetics, Univ. Minnesota, St. Paul, MN 55108
Donald L. Wyse
Affiliation:
Dep. Agron. and Plant Genetics, Univ. Minnesota, St. Paul, MN 55108
James D. Burton
Affiliation:
Dep. Agron. and Plant Genetics, Univ. Minnesota, St. Paul, MN 55108
David A. Somers
Affiliation:
Dep. Agron. and Plant Genetics, Univ. Minnesota, St. Paul, MN 55108
Burle G. Gengenbach
Affiliation:
Dep. Agron. and Plant Genetics, Univ. Minnesota, St. Paul, MN 55108
Get access Rights & Permissions [Opens in a new window]

Abstract

In greenhouse studies, the calculated I50 (herbicide application resulting in 50% inhibition of shoot regrowth) in tall fescue was approximately 0.004 kg/ha for both sethoxydim and haloxyfop. In red fescue, the I50 for sethoxydim and haloxyfop was 9.4 kg/ha and 0.04 kg/ha, respectively. As measured in crude cell extracts of tall fescue, incorporation of 14C-acetyl-coenzyme A into fatty acids was inhibited 62 and 71% by 10 μM sethoxydim and 10 μM haloxyfop, respectively. In red fescue, 10 μM haloxyfop inhibited 14C-acetyl-CoA incorporation into fatty acids by 29%, whereas 10 μM sethoxydim had no effect. The I50 for inhibition of acetyl-coenzyme A carboxylase activity in tall fescue with sethoxydim and haloxyfop was 6.9 and 5.8 μM, respectively. In red fescue the I50 for haloxyfop was 118 μM. Sethoxydim concentrations as high as 1 mM had little effect on acetyl-coenzyme A carboxylase activity in red fescue. These results suggest that acetyl-coenzyme A carboxylase is a sensitive site of action for sethoxydim and haloxyfop in tall fescue, and that tolerance to these herbicides in red fescue is due to the presence of a more tolerant form of the enzyme.

Keywords

Red fescue, Festuca rubra L. ♯3 FESRU, ‘Pennlawn’tall fescue, Festuca arundinacea Schreb. ♯ FESAR, ‘Kentucky 31′sethoxydim, 2-[1-(ethoxyimino)butyl]-5-[2-(ethylthio)propyl]-3-hydroxy-2-cyclohexen-1-onehaloxyfop, 2-[4-[[3-chloro-5-(trifluoromethyl)-2-pyridinyl]oxy]-phenoxy]propanoic acidacetyl-coenzyme A carboxylase (EC 6.4.1.2)Fatty acid biosynthesiscyclohexanedionearyloxyphenoxypropionateFESARFESRU
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 37 , Issue 4 , July 1989 , pp. 512 - 516
Copyright
Copyright © 1989 by the Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Buhler, D. D., Swisher, B. A., and Burnside, O. C. 1985. Behavior of 14C-haloxyfop-methyl in intact plants and cell cultures. Weed Sci. 33:291–299.Google Scholar
2. Burton, J. D., Gronwald, J. W., Somers, D. A., Connelly, J. A., Gengenbach, B. G., and Wyse, D. L. 1987. Inhibition of plant acetyl-coenzyme A carboxylase by the herbicides sethoxydim and haloxyfop. Biochem. Biophys. Res. Comm. 148:1039–1044.CrossRefGoogle ScholarPubMed
3. Butler, J.H.B. 1985. Sethoxydim herbicide in red fescue (Festuca rubra) and bentgrass (Agrostis tenuis), levels and mechanisms of selectivity. Ph.D. Thesis. Oregon State Univ. 58 pp.Google Scholar
4. Butler, J.H.B. and Appleby, A. P. 1986. Tolerance of red fescue (Festuca rubra) and bentgrass (Agrostis spp.) to sethoxydim. Weed Sci. 34:457–461.Google Scholar
5. Camp, P. J. and Randall, D. D. 1985. Purification and characterization of the pea chloroplast pyruvate dehydrogenase complex. Plant Physiol. 77:571–577.Google Scholar
6. Campbell, J. R. and Penner, D. 1985. Sethoxydim metabolism in monocotyledonous and dicotyledonous plants. Weed Sci. 33:771–773.Google Scholar
7. Cho, H.-Y, Widholm, J. M., and Slife, F. W. 1986. Effects of haloxyfop on corn (Zea mays) and soybean (Glycine max) cell suspension cultures. Weed Sci. 34:496–501.Google Scholar
8. Focke, M. and Lichtenthaler, H. K. 1987. Inhibition of the acetyl-CoA carboxylase of barley chloroplasts by cycloxydim and sethoxydim. Z. Naturforsch. 42c:1361–1363.Google Scholar
9. Hoj, P. B. and Mikkelsen, J. D. 1982. Partial separation of individual enzyme activities of an ACP-dependent fatty acid synthetase from barley chloroplasts. Carlsberg Res. Commun. 47:119–141.Google Scholar
10. Hoppe, H. H. and Zacher, H. 1985. Inhibition of fatty acid biosynthesis in isolated bean and maize chloroplasts by herbicidal phenoxy-phenoxypropionic acid derivatives and structurally related compounds. Pestic. Biochem. Physiol. 24:298–305.CrossRefGoogle Scholar
11. Hosaka, H., Inaba, H., and Ishikawa, H. 1984. Response of monocotyledons to BAS 9052 OH. Weed Sci. 32:28–32.Google Scholar
12. Kobek, K., Focke, M., and Lichtenthaler, H. K. 1988. Fatty acid biosynthesis and acetyl-CoA carboxylase as a target of diclofop, fenoxaprop and other aryloxy-phenoxy-propionic acid herbicides. Z. Naturforsch. 43c:47–54.Google Scholar
13. Kobek, K., Focke, M., Lichtenthaler, H. K., Retzlaff, G., and Wurzer, B. 1988. Inhibition of fatty acid biosynthesis in isolated chloroplasts by cycloxydim and other cyclohexane-1,3-diones. Physiol. Plant. 72:492–498.CrossRefGoogle Scholar
14. Morrison, W. R. and Smith, L. M. 1964. Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride-methanol. J. Lipid Res. 5:600–608.Google Scholar
15. Nikolau, B. J., Hawke, J. C., and Slack, C. R. 1981. Acetyl-coenzyme A carboxylase in maize leaves. Arch. Biochem. Biophys. 211:605–612.Google Scholar
16. Rendina, A. R. and Felts, J. M. 1988. Cyclohexanedione herbicides are selective and potent inhibitors of acetyl-CoA carboxylase from grasses. Plant Physiol. 86:983–986.Google Scholar
17. Rendina, A. R., Felts, J. M., Beaudoin, J. D., Craig- Kennard, A. C., Look, L. L., Paraskos, S. L., and Hagenah, J. A. 1988. Kinetic characterization, stereoselectivity and species selectivity of the inhibition of plant acetyl-CoA carboxylase by the aryloxyphenoxypropionic acid grass herbicides. Arch. Biochem. Biophys. 265:219–225.Google Scholar
18. Secor, J. and Cséke, C. 1988. Inhibition of acetyl-CoA carboxylase activity by haloxyfop and tralkoxydim. Plant Physiol. 86:10–12.Google Scholar
19. Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., and Klenk, D. C. 1985. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150:76–85.Google Scholar
20. Stoltenberg, D. E. 1988. Selectivity and mechanism of action of sethoxydim and haloxyfop. Ph.D. Thesis. Univ. Minnesota. 48 pp.Google Scholar
21. Swisher, B. A. and Corbin, F. T. 1982. Behavior of BAS-9052 OH in soybean (Glycine max) and johnsongrass (Sorghum halepense) plant and cell cultures. Weed Sci. 30:640–650.Google Scholar
22. Wyse, D. L., Elling, L. J., White, D. B., and McGraw, R. L. 1985. Quackgrass (Agropyron repens) control in red fescue seed production. Weed Sci. 34:94–97.Google Scholar