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Effect of Herbicides on the Growth of Chlorella and Bacillus using the Paper Disc Method

Published online by Cambridge University Press:  12 June 2017

V. M. Thomas Jr. ,
L. J. Buckley ,
J. D. Sullivan Jr.  and
Miyoshi Ikawa
V. M. Thomas Jr.
Affiliation:
Dep. of Biochem., Univ. of New Hampshire, Durham, NH 03824
L. J. Buckley
Affiliation:
Dep. of Biochem., Univ. of New Hampshire, Durham, NH 03824
J. D. Sullivan Jr.
Affiliation:
Dep. of Biochem., Univ. of New Hampshire, Durham, NH 03824
Miyoshi Ikawa
Affiliation:
Dep. of Biochem., Univ. of New Hampshire, Durham, NH 03824
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Abstract

Twenty herbicides were tested for activity in a paper disc agar bioassay. The growth of Chlorella pyrenoidosa Chick was inhibited by dinoseb (2-sec-butyl-4,6-dinitrophenol), diquat [6,7-dihydropyrido(1,2-a:2′,1′-c)pyrazinediium ion], paraquat (1,1′-dimetnyl-4,4′-bipyridinium ion), 3′,4′-dichloro-2-methylvaleranilide, propachlor (2-chloro-N-isopropylacetanilide), and linuron [3-(3,4-dichlorophenyl)-1-methoxy-1-methylurea]. The growth of Bacillus subtilis Cohn was inhibited by dinoseb, diquat, paraquat, and 3′,4′-dichloro-2-methylvaleranilide. This assay using C. pyrenoidosa or B. subtilis offers a convenient means for the testing of herbicides.

Type
Research Article
Information
Weed Science , Volume 21 , Issue 5 , September 1973 , pp. 449 - 451
Copyright
Copyright © 1973 Weed Science Society of America 

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References

Literature Cited

1. Adachi, M. and Hamada, K. 1971. Chlorella assay of the activity of herbicides. Zasso Kenkyu (Weed Research) 1970:54–58; Chem. Abstr. 76:42559 (1972) CrossRefGoogle Scholar
2. Addison, D. A. and Bardsley, C. E. 1968. Chlorella vulgaris assay of the activity of soil herbicides. Weed Sci. 16:427–429.CrossRefGoogle Scholar
3. Ashton, F. M., Bisalputra, T., and Risley, E. B. 1966. Effect of atrazine on Chlorella vulgaris . Amer. J. Bot. 53:217–219.CrossRefGoogle Scholar
4. Breazeale, F. W. and Camper, N. D. 1972. Effect of selected herbicides on bacterial growth rates. Appl. Microbiol. 23:431–432.CrossRefGoogle ScholarPubMed
5. Geoghagen, M. J. 1957. The effect of some substituted methylureas on the respiration of Chlorella vulgaris var. viridis . New Phytol. 56:71–80.CrossRefGoogle Scholar
6. Gramlich, J. V. and Frans, R. E. 1964. Kinetics of Chlorella inhibition by herbicides. Weeds 12:184–189.CrossRefGoogle Scholar
7. Hartman, P. A. 1968. Miniaturized microbiological methods. Academic Press, New York. 227 pp.Google Scholar
8. Helling, C. S., Kaufman, D. D., and Dieter, C. T. 1971. Algae bioassay detection of pesticide mobility in soils. Weed Sci. 19:685–690.CrossRefGoogle Scholar
9. Ikawa, M., Ma, D. S., Meeker, G. B., and Davis, R. P. 1969. Use of Chlorella in mycotoxin and phycotoxin research. J. Agr. Food Chem. 17:425–429.CrossRefGoogle Scholar
10. Jayaraman, A., Herbst, E. J., and Ikawa, M. 1968. The bioassay of aflatoxins and related substances with Bacillus megaterium spores and chick embryos. J. Amer. Oil Chem. Soc. 45:700–702.CrossRefGoogle ScholarPubMed
11. Kratky, B. A. and Warren, G. F. 1971. The use of three simple, rapid bioassays on forty-two herbicides. Weed Res. 11:257–262.Google Scholar
12. Kratochvil, E. E. 1951. Determinations of the effect of several herbicides on soil microorganisms. Weeds 1:25–31.CrossRefGoogle Scholar
13. Kruglov, Yu. V. 1970. Alalogical method of atrazine determination in soil. Izv. Akad. Nauk. SSSR, Ser. Biol. 1970:144–147; Chem. Abstr. 73:2847 (1970).Google Scholar
14. Leoppky, C. and Tweedy, B. G. 1969. Effect of selected herbicides upon growth of soil algae. Weed Sci. 17:110–113.CrossRefGoogle Scholar
15. Shennan, J. L. and Fletcher, W. W. 1965. The growth in vitro of micro-organisms in the presence of substituted phenoxyacetic acids and phenoxybutyric acids. Weed Res. 5:266–274.CrossRefGoogle Scholar
16. Sikka, H. C. and Pramer, D. 1968. Physiological effects of fluometuron on some unicellular algae. Weed Sci. 16:296–299.CrossRefGoogle Scholar
17. Stevenson, E. C. and Mitchell, J. W. 1945. Bacteriostatic and bactericidal properties of 2,4-dichlorophenoxy-acetic acid. Science 101:642–644.CrossRefGoogle Scholar
18. Sullivan, J. D. Jr. and Ikawa, M. 1972. Variations in inhibition of growth of five Chlorella strains by mycotoxins and other toxic substances. J. Agr. Food Chem. 20:921–922.CrossRefGoogle ScholarPubMed
19. Tsay, Sue-Fei, Lee, Jhy-Mei, and Lynd, J. Q. 1970. The interactions of Cu++ and CN- with paraquat phytotoxicity to a Chlorella . Weed Sci. 18:596–598.CrossRefGoogle Scholar
20. Vance, B. D. and Smith, D. L. 1962. Effects of five herbicides on three green algae. Texas J. Sci. 20:329–337.Google Scholar
21. Wolf, F. T. 1962. Growth inhibition of Chlorella induced by 3-amino-1,2,4-triazole, and its reversal by purines. Nature 193:901–902.CrossRefGoogle ScholarPubMed
22. Zweig, G., Hitt, J. E., and McMahon, R. 1968. Effect of certain quinones, diquat, and diuron on Chlorella pyrenoidosa Chick (Emerson strain). Weed Sci. 16:69–73.CrossRefGoogle Scholar