Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-25T06:40:16.793Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of glyphosate spray droplets on leaf cytology in velvetleaf (Abutilon theophrasti)

Published online by Cambridge University Press:  20 January 2017

Jan S. Ryerse ,
Roger A. Downer ,
R. Douglas Sammons  and
Paul C. C. Feng
Roger A. Downer
Affiliation:
Laboratory For Pest Control Application Technology, OARDC/OSU, 1680 Madison Avenue, Wooster, OH 44691
R. Douglas Sammons
Affiliation:
Monsanto Company, 700 Chesterfield Village Parkway North, Chesterfield, MO 63198
Paul C. C. Feng
Affiliation:
Monsanto Company, 700 Chesterfield Village Parkway North, Chesterfield, MO 63198
Get access Rights & Permissions [Opens in a new window]

Abstract

Leaf cytoarchitecture was evaluated by light microscopy and scanning electron microscopy, and cell viability was monitored by fluorescence after treatment of velvetleaf with defined concentrations and droplet sizes of formulated glyphosate and blended tallowamine surfactant. In response to droplets of formulated glyphosate larger than in field sprays but useful for studying structural change, we observe that the leaf epidermis thins and flattens within 1.5 h, the epidermal, mesophyll, and vascular cells at the contact site exhibit localized cytolysis by 6 h, and cytolysis and pycnosis remain restricted to the contact site at 24 h. Using endogeneous fluorescence as a marker for nonviable cells, it was determined that cellular changes are directly correlated with droplet size and that the changes are minimal after exposure to spray sizes and concentrations of formulated glyphosate and blended tallowamine typically used in the field. The results show that, at field use concentrations, the effect of formulated glyphosate and blended tallowamine on leaf cytoarchitecture is modest and localized but sufficient to allow herbicide entry.

Keywords

Glyphosatevelvetleaf, Abutilon theophrasti Medicus ABUTHBlended tallowaminefluorescencemicroscopyRoundup® Originalspray droplets
Type
Weed Management
Information
Weed Science , Volume 52 , Issue 2 , April 2004 , pp. 302 - 309
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

Casely, J. C. and Coupland, D. 1985. Environmental and plant factors affecting glyphosate uptake, movement and activity. Pages 92–123 in Grossbard, E. and Atkinson, D. eds. The Herbicide Glyphosate. London: Butterworth.Google Scholar
Denis, M. H. and Delrot, S. 1997. Effects of salt and surfactants on foliar uptake and long distance transport of glyphosate. Plant Physiol. Biochem 35:291–301.Google Scholar
De Ruiter, H., Uffing, A. J. M., and Meinen, E. 1996. Influence of surfactants and ammonium sulfate on glyphosate phytotoxicity to quackgrass (Elytigia repens). Weed Technol 10:803–808.CrossRefGoogle Scholar
De Ruiter, H., Uffing, A. J. M., Meinen, E., and Prins, A. 1990. Influence of surfactants and plant species on leaf retention of spray solutions. Weed Sci 38:567–572.CrossRefGoogle Scholar
Etheridge, R. E., Womac, A. R., and Mueller, T. C. 1999. Characterization of the spray droplet spectra and patterns of four venturi-type drift reducing nozzles. Weed Technol 13:765–770.CrossRefGoogle Scholar
Feng, P. C. C., Chiu, T., Sammons, R. D., and Ryerse, J. S. 2003. Droplet size affects glyphosate retention, absorption and translocation in corn. Weed Sci. 51:443–448.CrossRefGoogle Scholar
Feng, P. C. C., Ryerse, J. S., and Sammons, R. D. 1998. Correlation of leaf damage with uptake and translocation of glyphosate in velevetleaf (Abutilon theophrasti). Weed Technol 12:300–307.CrossRefGoogle Scholar
Gaskin, R. E. and Holloway, P. J. 1992. Some physicochemical factors influencing foliar uptake enhancement of glyphosate mono(isopro-pylammonium) by polyoxyethelene surfactants. Pestic. Sci 34:195–206.CrossRefGoogle Scholar
Kirkwood, R. C. and McKay, I. 1994. Accumulation and elimination of herbicides in selected crop and weed species. Pest. Sci 42:241–249.Google Scholar
Liu, S. H., Campbell, R. A., Studens, J. A., and Wagner, R. G. 1996. Absorption and translocation of glyphosate in Aspen (Populus tremuloides Michx.) as influenced by droplet size, droplet number, and herbicide concentration. Weed Sci 44:482–488.CrossRefGoogle Scholar
Nobel, P. S. 1991. Physicochemical and Environmental Plant Physiology. San Diego, CA: Academic. Pp. 473–520.CrossRefGoogle Scholar
Reichard, D. L. 1990. A system for producing various sizes, numbers and frequencies of uniform-size droplets. Trans. ASAE 33:1767–1770.CrossRefGoogle Scholar
Riechers, D. E., Wax, L. M., Liebl, R. A., and Bush, D. R. 1994. Surfactant-increased glyphosate uptake into plasma membrane vesicles isolated from common lambsquarters leaves. Plant Physiol 105:1419–1425.CrossRefGoogle ScholarPubMed
Ryerse, J. S., Feng, P. C. C., and Sammons, R. D. 2001. Endogenous fluorescence identifies dead cells in plants. Microsc. Today 1:22–24.CrossRefGoogle Scholar