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Do light acclimation mechanisms reduce the effects of light-dependent herbicides in duckweed (Lemna minor)?

Published online by Cambridge University Press:  20 January 2017

Unai Artetxe ,
Antonio Hernández ,
José I. García-Plazaola  and
José M. Becerril
Unai Artetxe
Affiliation:
Department of Plant Biology and Ecology, University of the Basque Country, Apdo 644, 48080 Bilbao, Spain
Antonio Hernández
Affiliation:
Department of Plant Biology and Ecology, University of the Basque Country, Apdo 644, 48080 Bilbao, Spain
José I. García-Plazaola
Affiliation:
Department of Plant Biology and Ecology, University of the Basque Country, Apdo 644, 48080 Bilbao, Spain
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Abstract

This research studies whether photoprotection mechanisms are able to counterbalance the short-term effect of two herbicides, acifluorfen methyl (AFM) and paraquat (PQ), that generate photo-oxidative stress in different subcellular locations. Duckweed plants grown under three light intensities (high-, medium-, and low light), and consequently expressing three levels of photoprotection, were exposed to both herbicides under the same light regime. Oxidative damage induced by AFM originated mainly from the cytosolic accumulation of protoporphyrin IX, leading to a process of plasma membrane disruption, a progressive and slow degradation of ascorbate and photosynthetic pigments, and glutathione accumulation. As most photoprotective mechanisms (antioxidants and xanthophylls-cycle-related energy dissipation) operate mainly within the chloroplast, these systems were unable to protect plants from AFM damage irrespective of the level of light acclimation. Paraquat effects developed more rapidly and to a greater extent than AFM in treated plants. Irrespective of the light intensity, the same sequence of degradation was observed: ascorbate followed by glutathione, α-tocopherol, pigments, and membrane disruption. In PQ-treated plants the generation of oxidative stress occurred mainly in the chloroplast, and cellular damage developed more slowly in highly photoprotected plants (high light); in fact, electrolyte leakage can be used as a marker for PQ tolerance. The effects of both herbicides indicate that the xanthophyll cycle is an early protective mechanism and confirms the central role of ascorbate in early photoprotection response. High levels of lipophilic and hydrophilic antioxidant contents did not lead to attenuated phytotoxicity of acifluorfen methyl and thus are not the basis to explain differential susceptibilities among duckweed plants.

Keywords

Acifluorfenparaquatduckweed, Lemna minor (L) LEMMIOxidative stressphotoprotectionxanthophyll cycle
Type
Physiology, Chemistry, Biochemistry
Information
Weed Science , Volume 54 , Issue 2 , April 2006 , pp. 230 - 236
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Amsellem, Z., Jansen, M., Dreisenaar, A., and Gressel, J. 1994. Developmental variability of photooxidative stress tolerance in paraquat-resistant Conyza . Plant Physiol 103:1097–1106.Google Scholar
Andrews, C. J., Cummins, I., Skipsey, M., Grundy, N. M., Jepson, I., Townson, J., and Edwards, R. 2005. Purification and characterisation of a family of glutathione transferases with roles in herbicide detoxification in soybean (Glycine max L.); selective enhancement by herbicides and herbicides safeners. Pest. Biochem. Physiol 82:205–219.Google Scholar
Artetxe, U., García-Plazaola, J. I., Hernández, A., and Becerril, J. M. 2002. Low light grown duckweed plants are more protected against the toxicity induced by Zn and Cd. Plant Physiol. Biochem 40:859–863.Google Scholar
Becerril, J. M. and Duke, S. O. 1989. Protoporphyrin IX content correlates with activity of photobleaching herbicides. Plant Physiol 90:1175–1181.Google Scholar
Becerril, J. M., Duke, M. V., Nandihalli, U. B., Matsumoto, H., and Duke, S. O. 1992. Light control of porphyrin accumulation in acifluorfen-methyl-treated Lemna pausicostata . Physiol. Plant 86:6–16.Google Scholar
Dayan, F. E., Weete, J. D., Duke, S. O., and Hancock, H. G. 1997. Soybean (Glycine max) cultivar differences in response to sulfentrazone. Weed Sci 45:634–641.Google Scholar
Demmig-Adams, B. and Adams, W. W. 1996. The role of xanthophyll cycle carotenoids in the protection of photosynthesis. Trends Plant Sci 1:21–26.Google Scholar
DeRidder, B. P., Dixon, D. P., Beusmann, D. J., Edwards, R., and Goldsbrough, P. B. 2002. Induction of glutathione S-transferases in Arabidopsis by herbicide safeners. Plant Physiol 130:1497–1505.CrossRefGoogle ScholarPubMed
Donahue, J. L., Okpodu, C. M., Cramer, C. L., Grabau, E. A., and Alscher, R. G. 1997. Responses of antioxidants to paraquat in pea leaves. Plant Physiol 113:249–257.Google Scholar
Duke, S. O., Lydon, J., Becerril, J. M., Sherman, T. D., Lehnen, L. P., and Matsumoto, H. 1991. Protoporphyrinogen oxidase-inhibiting herbicides. Science 39:465–473.Google Scholar
Foyer, C. H., Lelandais, M., and Kunert, K. J. 1994. Photooxidative stress in plants. Physiol. Plant 92:696–717.Google Scholar
Foyer, C. H. and Noctor, G. 2005. Oxidant and antioxidant signalling in plants: a re-evaluation of the concept of oxidative stress in a physiological context. Plant Cell Environ 28:1056–1071.Google Scholar
Fryer, M. J. 1992. The antioxidant effects of thylakoid Vitamin E (α-tocopherol). Plant Cell Environ 15:381–392.Google Scholar
García-Plazaola, J. I., Artetxe, U., and Becerril, J. M. 1999. Diurnal changes in antioxidant and carotenoid composition in the Mediterranean schlerophyll tree Quercus ilex (L.) during winter. Plant Sci 143:125–133.Google Scholar
García-Plazaola, J. I. and Becerril, J. M. 1999. A rapid HPLC method to measure lipophilic antioxidants in stressed plants: simultaneous determination of carotenoids and tocopherols. Phytochem. Anal 10:1–7.Google Scholar
García-Plazaola, J. I. and Becerril, J. M. 2000. Effects of drought on photoprotective mechanisms in European beech (Fagus sylvatica L.) seedlings from different provenances. Trees 14:485–490.Google Scholar
García-Plazaola, J. I. and Becerril, J. M. 2001. Seasonal changes in photosynthetic pigments and antioxidants in beech (Fagus sylvatica) in a mediterranean climate: implications for tree decline diagnosis. Australian J. Plant Physiol 28:225–232.Google Scholar
García-Plazaola, J. I., Hernandez, A., Artetxe, U., and Becerril, J. M. 2002. Regulation of the xanthophyll cycle pool size in duckweed (Lemna minor) plants. Physiol. Plant 116:121–126.Google Scholar
Griffith, O. W. 1980. Glutathione and glutathione disulphide. Anal. Biochem 106:207–212.Google Scholar
Gullner, G. and Dodge, A. D. 2000. Effect of singlet oxygen generating substances on the ascorbic acid and glutathione content in pea leaves. Plant Sci 154:127–133.Google Scholar
Hart, J. J. and DiTomaso, J. M. 1994. Sequestration and oxygen radical detoxification as mechanisms of paraquat resistance. Weed Sci 42:277–284.Google Scholar
Hess, F. D. 2000. Light-dependent herbicides: an overview. Weed Sci 48:160–170.Google Scholar
Holt, J. S. 1993. Mechanisms and agronomic aspects of herbicide resistance. Annu. Rev. Plant Physiol. Plant Molec. Biol 4:203–229.Google Scholar
Iturbe-Ormaetxe, I., Escuredo, P. R., Arrese-Igor, C., and Becana, M. 1998. Oxidative damage in pea plants exposed to water deficit or paraquat. Plant Physiol 116:173–181.Google Scholar
Kenyon, W. H. and Duke, S. O. 1985. Effects of acifluorfen on endogenous antioxidants and protective enzymes in cucumber (Cucumis sativus L.) cotyledons. Plant Physiol 79:862–866.Google Scholar
Kim, J. H. and Lee, C. H. 2005. In vivo deleterious effects specific to reactive oxygen species on photosystem I and II after photo-oxidative treatments of rice (Oryza sativa L.) leaves. Plant Sci 168:1115–1125.CrossRefGoogle Scholar
Lee, H. J. and Duke, S. O. 1994. Protoporphyringen oxidizing activities involved in the mode of action of peroxidizing herbicides. J. Agric. Food. Chem 42:2610–2618.Google Scholar
Lehnen, L. P., Sherman, T. D., Becerril, J. M., and Duke, S. O. 1990. Tissue and cellular localization of acifluorfen-induced porphyrins in Cucumber cotyledons. Pestic. Biochem. Physiol 37:239–248.Google Scholar
Mano, J., Hideg, E., and Asada, K. 2004. Ascorbate in thylakoid lumen functions as an alternative electron donor to photosystem II and photosystem I. Arch. Biochem. Biophys 429:71–80.Google Scholar
Sherman, T. D., Becerril, J. M., Matsumoto, H., Duke, M. V., Jacobs, J. M., Jacobs, N. J., and Duke, S. O. 1991. Physiological basis for differential sensitivities of plant species to protoporphyrinogen oxidase-inhibiting herbicides. Plant Physiol 97:280–287.Google Scholar
Streb, P., Shang, W., and Feierabend, J. 1999. Resistance of cold-hardened winter rye leaves (Secale cereale L.) to photo-oxidative stress. Plant Cell Environ 22:1211–1223.Google Scholar
Tanielian, C. and Wolff, C. 1988. Mechanism of physical quenching of singlet molecular oxygen by chlorophylls and related compounds of biological interest. Photochem. Photobiol 48:277–280.Google Scholar
Váradi, G., Darkó, E., and Lehoczki, E. 2000. Changes in the xanthophyll cycle and fluorescence quenching indicate light-dependent early events in the action of paraquat and the mechanism of resistance to paraquat in Errigeron canadensis (L.) Cronq. Plant Physiol 123:1459–1469.Google Scholar
Ye, B., Faltin, Z., Ben-Hayyim, G., Eshdat, Y., and Gressel, J. 2000. Correlation of glutathione peroxidase to paraquat/oxidative stress resistance in Conyza determined by direct fluorometric assay. Pestic. Biochem. Physiol 66:182–194.Google Scholar