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A detoxification gene in transgenic Nicotiana tabacum confers 2,4-D tolerance

Published online by Cambridge University Press:  12 June 2017

David I. Last  and
Danny J. Llewellyn
David I. Last
Affiliation:
CSIRO Division of Plant Industry, P.O. Box 1600, Canberra City, A.C.T. 2601, Australia
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Abstract

Transgenic Nicotiana tabacum with tolerance to 2,4-D has previously been produced using a bacterial 2,4-D-dioxygenase gene (tfdA) driven by the 35S promoter of cauliflower mosaic virus. Using promoters from the Pisum sativum plastocyanin gene (petE) and an Arabidopsis thaliana histone gene (H4A), we demonstrate that similar protection from 2,4-D can be obtained in transgenic N. tabacum by targeting expression of tfdA to either meristematic tissues or chloroplast-containing tissues. As with the 35S promoter constructs, the plants are tolerant but not completely resistant; very young seedlings in particular are only slightly protected. However, the levels of tolerance observed could offer a useful degree of protection from accidental spray drift.

Keywords

2,4-DNicotiana tabacum L., tobaccoPisum sativum L., peaCrop injuryherbicide resistancetargeted gene expressiontransgenic plantspromoter
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 47 , Issue 4 , August 1999 , pp. 401 - 404
Copyright
Copyright © 1999 by the Weed Science Society of America 

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