Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-24T12:00:35.758Z Has data issue: false hasContentIssue false
  • English
  • Français

Cross-Resistance in Fluridone-Resistant Hydrilla to Other Bleaching Herbicides

Published online by Cambridge University Press:  20 January 2017

Atul Puri ,
William T. Haller  and
Michael D. Netherland
Atul Puri*
Affiliation:
Center for Aquatic and Invasive Plants, Institute of Food and Agricultural Sciences, University of Florida, P.O. Box 110610, Gainesville, FL 32611
William T. Haller
Affiliation:
Center for Aquatic and Invasive Plants, Institute of Food and Agricultural Sciences, University of Florida, P.O. Box 110610, Gainesville, FL 32611
Michael D. Netherland
Affiliation:
Center for Aquatic and Invasive Plants, Institute of Food and Agricultural Sciences, University of Florida, P.O. Box 110610, Gainesville, FL 32611
*
Corresponding author's E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The development of fluridone resistance by hydrilla has significantly impacted hydrilla management, and research is ongoing to develop alternate herbicides for effective hydrilla control. We determined the potential cross-resistance in fluridone-resistant hydrilla to other bleaching herbicides norflurazon, mesotrione, and topramezone-methyl. Phytoene, β-carotene, and chlorophyll contents as a function of hydrilla biotype and herbicide treatment were evaluated. Hydrilla shoot tips were collected from fluridone-susceptible (S) and -resistant (R) biotypes and exposed to 5, 25, 50, 75, and 100 µg L−1 of herbicide. The susceptible biotype showed an increase in phytoene and a decrease in β-carotene and chlorophyll contents when treated with 5 µg L−1 fluridone, whereas higher doses of fluridone were required to affect these pigments in the resistant biotype. There was no difference in response by S and R biotypes to mesotrione and topramezone-methyl, with both biotypes showing significant affects on pigment contents at 5 µg L−1. Higher doses of norflurazon were required to affect these pigments in the R compared to the S biotype. The S biotype had EC50 values of 11.7, 12.2, and 4.7 µg L−1, whereas the R biotype had EC50 values of 56.6, 41.1, and 41.7 µg L−1 fluridone for phytoene, β-carotene, and chlorophyll contents, respectively. There was no difference in EC50 for phytoene, β-carotene, and chlorophyll values between the hydrilla biotypes for mesotrione and topramezone-methyl herbicides. In fluridone-susceptible and -resistant hydrilla biotypes, EC50 values for phytoene, β-carotene, and chlorophyll were 12.4 to 11.8, 10.2 to 13.2, and 3.1 to 4.6 µg L−1 mesotrione and 12.6 to 13.5, 13.3 to 11.9, and 4.6 to 5.7 µg L−1 topramezone-methyl, respectively. For norflurazon, S and R biotypes had EC50 values of 33.1, 45.4, and 40.6 µg L−1 and 84.6, 81.0, and 92.7 µg L−1 for phytoene, β-carotene, and chlorophyll, respectively. These studies confirmed negative cross-resistance of fluridone-resistant hydrilla to mesotrione and topramezone-methyl and a positive cross-resistance to norflurazon.

Keywords

Fluridonemesotrionenorflurazontopramezone-methylhydrilla, Hydrilla verticillata (L.f.) Royle HYLLI.Invasive speciesherbicide resistanceaquatic weedpigment content
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 57 , Issue 5 , October 2009 , pp. 482 - 488
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

Arias, R. S., Dayan, F. E., Michel, A., Howell, J'Lynn, and Scheffler, B. E. 2006. Characterization of a higher plant herbicide-resistant phytoene desaturase and its use as a selectable marker. Plant Biotech. J. 4:263–273.Google Scholar
Arnon, D. I. 1949. Copper enzymes in isolated chloroplasts. Plant Physiol. 24:1–15.Google Scholar
Babczinski, P., Sandmann, G., Schmidt, R. R., Shiokawa, K., and Yasui, K. 1995. Substituted tetrahydropyrimidinones: a new herbicidal class of compounds inducing chlorosis by inhibition of phytoene desaturation. Pestic. Biochem. Physiol. 52:33–44.Google Scholar
Bartels, P. G. and Watson, C. W. 1978. Inhibition of carotenoid synthesis by fluridone and norflurazon. Weed Sci. 26:198–203.Google Scholar
Blackburn, R. D., Weldon, L. W., Yeo, R. R., and Taylor, T. M. 1969. Identification and distribution of similar-appearing aquatic weeds in Florida. J. Aquatic Plant Manag. 8:11–21.Google Scholar
Böger, P. and Sandmann, G. 1998. Carotenoid biosynthesis inhibitor herbicides—mode of action and resistance mechanisms. Pesticide Outlook. 9:29–35.Google Scholar
Chamovitz, D., Sandmann, G., and Hirschberg, J. 1993. Molecular and biochemical characterization of herbicide-resistant mutants of cyanobacteria reveals that phytoene desaturation is a rate-limiting step in carotenoid biosynthesis. J. Biol. Chem. 268:17348–17353.Google Scholar
Crouch, N. P., Adlington, R. M., Baldwin, J. E., Lee, M. H., and Mackinnon, C. H. 1997. A mechanistic rationalization for the substrate specificity of recombinant mammalian 4-hydroxy-phenylpyruvate dioxygenase (4-HPPD). Tetrahedron. 53:6993–7010.Google Scholar
Doong, R. L., MacDonald, G. E., and Shilling, D. G. 1993. Effect of fluridone on chlorophyll, carotenoids and anthocyanin content of hydrilla. J. Aquat. Plant Manag. 31:55–59.Google Scholar
Duke, S. O., Kenyon, W. H., and Paul, R. N. 1985. FMC 57020 effects on chloroplast development in pitted morningglory (Ipomoea lacunosa) cotyledons. Weed Sci. 33:786–794.Google Scholar
Hiscox, J. D. and Israelstam, G. F. 1979. A method for the extraction of chlorophyll from leaf tissue without maceration. Can. J. Bot. 57:1332–1334.Google Scholar
Koschnick, T. J., Haller, W. T., and Netherland, M. D. 2006. Aquatic plant resistance to herbicides. Aquatics. 28:4–6.Google Scholar
Lee, D. L., Prisbylla, M. P., Cromartic, T. H., Dagarin, D. P., Howard, S. W., Provan, W. M., Ellis, M. K., Fraser, T., and Mutter, L. C. 1997. The discovery of structural requirements of inhibitors of p-hydroxyphenylpyruvate dioxygenase. Weed Sci. 45:601–609.Google Scholar
Mayer, M. P., Nievelstein, V., and Beyer, P. 1992. Purification and characterization of a NADPH dependent oxidoreductase from chromoplast of Narcissus pseudonarcissus: a redox-mediator possibly involved in carotene desaturation. Plant Physiol. Biochem. 30:389–398.Google Scholar
Michel, A., Scheffler, B. E., Arias, R. S., Duke, S. O., Netherland, M. D., and Dayan, F. E. 2004. Somatic mutation-mediated evolution of herbicide resistance in the non-indigenous invasive plant hydrilla (Hydrilla verticillata). Mol. Ecol. 13:3229–3237.Google Scholar
Netherland, M. D., Getsinger, K. D., and Skogerboe, J. D. 1997. Mesocosm evaluation of the species-selective potential of fluridone. J. Aquat. Plant Manag. 35:41–50.Google Scholar
Norris, S. R., Barrette, T., and DellaPenna, D. 1995. Genetic dissection of carotenoid synthesis in arapidopsis defines plastoquinone as an essential component of phytoene desaturation. Plant Cell. 7:2139–2149.Google Scholar
Pallett, K. E., Little, J. P., Sheekey, M., and Veerasekaran, P. 1998. The mode of action of isoxaflutole. I. Physiological effects, metabolism, and selectivity. Plant Physiol. Biochem. 62:113–124.Google Scholar
Prysbilla, M. P., Onisko, B. C., Shribbs, J. M., Adams, D. O., Liu, Y., Ellis, M. K., Hawkes, T. R., and Mutter, L. C. 1993. The novel mechanism of action of the herbicidal triketones. Proceedings Brighton Crop Protection Conference Weeds. 2:731–738.Google Scholar
Puri, A., MacDonald, G. E., Singh, M., and Haller, W. T. 2006. Phytoene and β-carotene response of fluridone-susceptible and -resistant hydrilla (Hydrilla verticillata) biotypes to fluridone. Weed Sci. 54:995–999.Google Scholar
Sandmann, G. and Böger, P. 1983. Comparison of the bleaching activity of norflurazon and oxyfluorfen. Weed Sci. 31:338–341.Google Scholar
Sandmann, G. and Böger, P. 1989. Inhibition of carotenoid biosynthesis by herbicides. Pages 25–44. In Böger, P. and Sandmann, G. Target Sites of Herbicide Action. Boca Raton, FL CRC.Google Scholar
Sprecher, S. L., Netherland, M. D., and Stewart, A. B. 1998. Phytoene and carotene response of aquatic plants to fluridone under laboratory conditions. J. Aquat. Plant Manag. 36:111–120.Google Scholar