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Behavior of BAS-9052 OH in Soybean (Glycine max) and Johnsongrass (Sorghum halepense) Plant and Cell Cultures

Published online by Cambridge University Press:  12 June 2017

Beth A. Swisher  and
Frederick T. Corbin
Beth A. Swisher
Affiliation:
Dep. Crop Sci., North Caroline State Univ., Raleigh, NC 27650
Frederick T. Corbin
Affiliation:
Dep. Crop Sci., North Caroline State Univ., Raleigh, NC 27650
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Abstract

Research involved the behavior of BAS-9052 OH {2[1-(ethoxyimino)butyl]-5-[2(ethylthio)propyl]-3-hydroxy-2-cyclohexen-1-one} in soybean [Glycine max (L.) Merr.] and johnsongrass [Sorghum halepense (L.) Pers.] plants, and the fate of 14C-BAS-9052 OH in intact plants and cell cultures of both species. Microscopic examination of seedling johnsongrass plants (two- to three-leaf stage) treated with foliar applications of 0.48 μg/plant revealed disorganized apical regions and necrotic cells within the apex and leaf primordia of the shoot. Necrotic zones were also evident at the base of expanding leaves and in root apices 1 day after treatment. Following application of 14C-BAS-9052 OH, the same radioactive products were isolated from cell cultures and intact plants. Products included BAS-9052 OH and three unidentified metabolites. Greater proportions of unchanged BAS-9052 OH were extracted from the apical leaves, roots, and cell cultures of johnsongrass than of soybean. BAS-9052 OH was thermal and photo-labile, and a large portion of 14C may not have entered the plant as BAS-9052 OH. However, tolerant soybean plants and cell cultures appeared to have metabolized the herbicide more rapidly than susceptible johnsongrass plants and cell cultures. An average of 64% of the 14C remained in the treated leaf of johnsongrass compared with 48% in soybean. About one-half as much 14C was translocated to the apical leaves of susceptible johnsongrass than tolerant soybean. Therefore, differential uptake and translocation cannot account for the selectivity of BAS-9052 OH.

Keywords

Herbicide metabolismmode of actiontissue culturesethoxydim
Type
Research Article
Information
Weed Science , Volume 30 , Issue 6 , November 1982 , pp. 640 - 650
Copyright
Copyright © 1982 by the Weed Science Society of America 

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References

Literature Cited

1. Ashton, F. M. and Crafts, A. S. 1981. Mode of action of herbicides. 2nd. Ed. John Wiley and Sons, New York. 525 pp.Google Scholar
2. Campbell, J. R. and Penner, D. 1980. Nonbiological transformations of BAS-9052 OH. Proc. North Cent. Weed Control Conf. 35:17.Google Scholar
3. Campbell, J. R. and Penner, D. 1981. Absorption and translocation of BAS-9052 OH. Abstr., Weed Sci. Soc. Am. p. 109.Google Scholar
4. Cutter, E. G. 1971. Plant Anatomy: Experimentation and Interpretation. Part 2. Organs. Addison-Wesley, Boston. 341 pp.Google Scholar
5. Davis, D. G., Mullins, J. S., Stolzenberg, G. E., and Booth, G. D. 1979. Permeation of organic molecules of widely differing solubilities and of water through isolated cuticles or orange leaves. Pestic. Sci. 10:19–31.CrossRefGoogle Scholar
6. Davis, D. G. and Shimabukuro, R. H. 1980. Studies of herbicide toxicity and mode of action using isolated mesophyll cells and callus-derived cell suspensions. Can. J. Bot. 58:1482–1489.Google Scholar
7. Esau, K. 1977. The Anatomy of Seed Plants. 2nd Ed. John Wiley and Sons, New York. 550 pp.Google Scholar
8. Fehr, W. R., Caviness, C. E., Burmood, D. T., and Pennington, J. S. 1971. Stage of development descriptions for soybeans. Crop Sci. 11:929–931.CrossRefGoogle Scholar
9. Gamborg, O. L., Miller, R. A., and Ojima, K. 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50:151–158.CrossRefGoogle ScholarPubMed
10. Gressel, J. 1979. A review of the place of in vitro cell culture systems in studies of action, metabolism and resistance of biocides affecting photosynthesis. Z. Naturforsch. 34c:905–913.CrossRefGoogle Scholar
11. Hashimoto, Y., Ishihara, K., and Soeda, Y. 1979. Fate of alloxy-dimsodium on or in soybean plants. Pestic. Sci. 4:299–304.CrossRefGoogle Scholar
12. Hess, F. D. 1980. A Chlamydomonas algal bioassay for detecting growth inhibitor herbicides. Weed Sci. 28:515–521.CrossRefGoogle Scholar
13. Hirono, Y. and Iwataki, I. 1979. The chemical structure and herbicidial activity of alloxydim-sodium and related compounds. Pages 235–243 in Geissbuhler, H., Brooks, G. T., and Kearney, P. C., eds. Advances in Pesticide Science. Part 3. Pergamon Press, Oxford.Google Scholar
14. Hoagland, D. R. and Arnon, D. I. 1950. The water culture method for growing plants without soil. Calif. Agric. Exp. Stn. Circ. 347:1–32.Google Scholar
15. Ishikawa, H., Okunuki, S., Kawana, T., and Hirono, Y. 1980. Histological investigations on the herbicidal effects of alloxy-dimsodium in pat. Pestic. Sci. 5:549–551.Google Scholar
16. Martin, J. T. and Juniper, B. E. 1970. The Cuticles of Plants. St. Martins Press, New York. 347 pp.Google Scholar
17. Oswald, T. H., Smith, A. E., and Phillips, D. V. 1978. Phytotoxicity and detoxification of metribuzin in dark-grown suspension cultures of soybean. Pestic. Biochem. Physiol. 8:73–83.CrossRefGoogle Scholar
18. Salisbury, F. B. and Ross, C. 1978. Plant Physiology. 2nd Ed. Wadsworth Publishing Co., Belmont, CA. 422 pp.Google Scholar
19. Soeda, V., Ishihara, K., Iwataki, I., and Kamimura, H. 1979. Fate of a herbicide 14C-alloxydim-sodium in sugar beet. Pestic. Sci. 4:121–128.CrossRefGoogle Scholar
20. Zilkah, S., Bocion, P. F., and Gressel, J. 1978. Target tissue for napropamide inhibition: Effects on green and white callus cultures and seedlings. Weed Sci. 26:711–713.Google Scholar
21. Zilkah, S. and Gressel, J. 1977. Cell cultures vs. whole plants for measuring phytotoxicity. III. Correlation between phytotoxicities in cell suspension cultures, calli and seedlings. Plant Cell Physiol. 18:815–820.Google Scholar