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Response properties of long-range axon-bearing amacrine cells in the dark-adapted rabbit retina

Published online by Cambridge University Press:  02 June 2009

W. Rowland Taylor
W. Rowland Taylor
Affiliation:
Neuroanatomische Abteilung, Max-Planck-Institut für Hirnforschung, Deutschordenstrasse 46, D-60528 Frankfurt, Germany
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Abstract

Axon-bearing amacrine cells in mammalian retinae are encountered relatively infrequently during electrophysiological investigations, and thus very little is known about their physiological properties. Patch-clamp electrodes were used to record light responses from two axon-bearing amacrine cells in flat-mounted, dark-adapted rabbit retina. The recorded cells were stained, and the morphology visualized. Both cells were capable of generating action potentials. In one case, a linear relationship between mean depolarization and action-potential frequency was demonstrated. The cells had a proximal dendritic arbor and a morphologically distinct, much larger axon terminal system. The receptive field of the center response was coextensive with the dendritic arbor, and thus also much smaller than the axon terminal system. The center response was suppressed by activation of an inhibitory surround. Both cells responded to center illumination with an inward current which became more transient as the size of the illuminating spot was increased. It is suggested that axon-bearing amacrine cells receive input over a receptive field defined by the dendritic arbor, and distribute their output over a much more extensive axon terminal system, most probably via action potentials.

Keywords

ElectrophysiologyAnatomyMammalian retinaAxon-bearing amacrine cellLight responseVoltage clamp
Type
Research Articles
Information
Visual Neuroscience , Volume 13 , Issue 4 , July 1996 , pp. 599 - 604
Copyright
Copyright © Cambridge University Press 1996

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References

Ames, A.I. & Nesbett, F.B. (1981). In vitro retina as an experimental model of the central nervous system. Journal of Neurochemistry 37, 867–877.CrossRefGoogle ScholarPubMed
Ammermüller, J. & Weiler, R. (1988). Physiological and morphological characterization of OFF-center amacrine cells in the turtle retina. Journal of Comparative Neurology 273, 137–148.CrossRefGoogle ScholarPubMed
Baylor, D.A. & Fettiplace, R. (1979). Synaptic drive and impulse generation in ganglion cells of turtle retina. Journal of Physiology 288, 107–127.CrossRefGoogle ScholarPubMed
Bloomfield, S.A. (1992). Relationship between receptive field and dendritic field size of amacrine cells in the rabbit retina. Journal of Neurophysiology 68, 711–725.CrossRefGoogle ScholarPubMed
Brecha, N., Oyster, C.W. & Takahashi, E.S. (1984). Identification and characterization of tyrosine hydroxylase immunoreactive amacrine cells. Investigative Ophthalmology 25, 66–70.Google ScholarPubMed
Cook, P. & Werblin, F. (1994). Spike initiation and propagation in wide field transient amacrine cells of the salamander retina. Journal of Neuroscience 14, 3852–3861.CrossRefGoogle ScholarPubMed
Dacey, D.M. (1989). Axon-bearing amacrine cells of the macaque monkey retina. Journal of Comparative Neurology 284, 275–293.CrossRefGoogle ScholarPubMed
Dacey, D.M. (1990). The dopaminergic amacrine cell. Journal of Comparative Neurology 301, 461–489.CrossRefGoogle ScholarPubMed
Famiglietti, E.V. (1992). Polyaxonal amacrine cells of rabbit retina: Size and distribution of PA1 cells. Journal of Comparative Neurology 316, 406–421.CrossRefGoogle ScholarPubMed
Granit, R., Kernell, D. & Shortess, G.K. (1963). Quantitative aspects of repetitive firing of mammalian motoneurones, caused by injected currents. Journal of Physiology 168, 911–931.CrossRefGoogle ScholarPubMed
Horikawa, K. & Armstrong, W.E. (1988). A versatile means of intracellular labeling: Injection of biocytin and its detection with avidin conjugates. Journal of Neuroscience Methods 25, 1–11.CrossRefGoogle ScholarPubMed
Huba, R., Schneider, H. & Hofmann, H.-D. (1992). Voltage-gated current of putative GABAergic amacrine cells in primary cultures and in retinal slice preparations. Brain Research 577, 10–18.CrossRefGoogle ScholarPubMed
Kolb, H. & Nelson, R. (1985). Functional neurocircuitry of amacrine cells in the cat retina. In Neurocircuitry of the Retina, A Cajal Memorial, ed. Gallego, A. & Gouras, P., pp. 215–232. New York: Elsevier Science Publishing.Google Scholar
Kolb, H., Nelson, R. & Mariani, A. (1981). Amacrine cells, bipolar cells and ganglion cells of the cat retina: A Golgi study. Vision Research 21, 1081–1114.CrossRefGoogle ScholarPubMed
Magherini, P.C. & Precht, W. (1976). Electrical properties of frog motoneurons in the in situ spinal cord. Journal of Neurophysiology 39, 459–473.CrossRefGoogle ScholarPubMed
Nelson, R. & Kolb, H. (1985). A17: A broad-field amacrine cell in the rod system of the cat retina. Journal of Neurophysiology 54, 592–614.CrossRefGoogle ScholarPubMed
Rickman, D.W. & Brecha, N.C. (1989). Morphologies of somatostatin-immunoreactive neurons in the rabbit retina. In Neurobiology of the Inner Retina, ed. Weiler, R. & Osborne, N.N., pp. 461–468. Berlin: Springer-Verlag.CrossRefGoogle Scholar
Sagar, S.M. (1987). Somatostatin-like immunoreactive material in the rabbit retina: Immunohistochemical staining using monoclonal antibodies. Journal of Comparative Neurology 266, 291–299.CrossRefGoogle ScholarPubMed
Taylor, W.R. & Wässle, H. (1995). Receptive field properties of starburst cholinergic amacrine cells in the rabbit retina. European Journal of Neuroscience 7, 2308–2321.CrossRefGoogle ScholarPubMed
Vaney, D.I. (1986). Morphological identification of serotonin-accumulating neurons in the living retina. Science 233, 444–446.CrossRefGoogle ScholarPubMed
Vaney, D.I. (1992). Photochromic intensification of diaminobenzidine reaction product in the presence of tetrazolium salts: Applications for intracellular labelling and immunohistochemistry. Journal of Neuroscience Methods 44, 217–223.CrossRefGoogle ScholarPubMed
Vaney, D.I., Peichl, L. & Boycott, B.B. (1988). Neurofibrillar longrange amacrine cells in mammalian retinae. Proceedings of the Royal Society B (London) 235, 203–219.Google ScholarPubMed
Voigt, T. & Wässle, H. (1987). Dopaminergic innervation of A II amacrine cells in mammalian retina. Journal of Neuroscience 7, 115–128.CrossRefGoogle ScholarPubMed
Wässle, H. & Boycott, B.B. (1991). Functional architecture of the mammalian retina. Physiological Reviews 71, 447–480.CrossRefGoogle ScholarPubMed
Wässle, H., Voigt, T. & Patel, B. (1987). Morphological and immunocytochemical identification of indolamine-accumulating neurons in the cat retina. Journal of Neuroscience 7, 1574–1585.CrossRefGoogle Scholar