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Packaging of rhodopsin and porphyropsin in the compound eye of the crayfish

Published online by Cambridge University Press:  02 June 2009

Joel Zeiger  and
Timothy H. Goldsmith
Joel Zeiger
Affiliation:
Department of Biology, Yale University, New Haven
Timothy H. Goldsmith
Affiliation:
Department of Biology, Yale University, New Haven
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Abstract

The distribution of 3-dehydroretinal (Ral2) in dorsal, middle, and ventral slices of eyes of the crayfish Procambarus clarkii was examined by HPLC. No pronounced differences were found. Similar results were obtained when the eyes were cut into anterior, intermediate, and posterior portions.

Dichroic difference spectra were measured in single halves of microvillar layers of isolated rhabdoms and the proportions of rhodopsin (P1) and porphyropsin (P2) estimated by comparison with computer-generated mixtures of these pigments, whose spectra are known from previous work. The fraction of visual pigment that is porphyropsin appears to be uniform throughout individual retinular cells and among the retinular cells of individual rhabdoms, but various substantially among different rhabdoms from the same eye.

The interommatidial variation in the amount of P2 greatly exceeds the gross regional variation in Ral2. This means there is an intermingling of ommatidia with different levels of P2. The variability in P2 among ommatidia is not likely to have important implications for the vision of the crayfish but suggests that in the metabolism of retinoids, individual ommatidia are quasi-independent metabolic units. The results are compatible with a single opsin for both crayfish rhodopsin and porphyropsin.

Keywords

Porphyropsin3-DehydroretinalCrayfishMicrospectrophotometry
Type
Research Article
Information
Visual Neuroscience , Volume 10 , Issue 2 , March 1993 , pp. 193 - 202
Copyright
Copyright © Cambridge University Press 1993

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References

Beatty, D.D. (1975). Visual pigments of the American eel Anguilla rostrata. Vision Research 15, 771–776.Google Scholar
Bowmaker, J.K., Dartnall, H.J.A. & Herring, P.J. (1988). Longwave-sensitive visual pigments of some deep-sea fishes: Segregation of “paired” rhodopsins and porphyropsins. Journal of Comparative Physiology A 163, 685–698.CrossRefGoogle Scholar
Bridges, C.D.B. (1970). Reversible visual pigment changes in tadpoles exposed to light and darkness. Nature 227, 956–957.CrossRefGoogle ScholarPubMed
Bridges, C.D.B. (1972). The rhodopsin-porphyropsin visual system. In Handbook of Sensory Physiology, VII/1, Photochemistry of Vision, ed. Dartnall, H.J.A., pp. 417–480. Heidelberg: Springer-Verlag.Google Scholar
Cronin, T.W. & Goldsmith, T.H. (1981). Fluorescence of crayfish metarhodopsin studied in single rhabdoms. Biophysical Journal 35, 653–664.Google Scholar
Cronin, T.W. & Goldsmith, T.H. (1982). Photosensitivity spectrum of crayfish rhodopsin measured using fluorescence of metarhodopsin. Journal of General Physiology 79, 313–332.CrossRefGoogle ScholarPubMed
Cummins, D. & Goldsmith, T.H. (1981). Cellular identification of the violet receptor in the crayfish eye. Journal of Comparative Physiology 142, 199–202.Google Scholar
Dartnall, H.J.A., Lander, M.R. & Munz, F.W. (1961). Periodic changes in the visual pigment of a fish. In Progress in Photobiology, ed. Christensen, B. & Buchmann, B., pp. 203–213. Amsterdam: Elsevier.Google Scholar
Eguchi, E. & Waterman, T.H. (1979). Longterm dark induced fine structural changes in crayfish photoreceptor membrane. Journal of Comparative Physiology 131, 191–203.CrossRefGoogle Scholar
Goldsmith, T.H. (1978 a). The spectral absorption of crayfish rhabdoms: Pigment, photoproduct and pH sensitivity. Vision Research 18, 463–473.CrossRefGoogle ScholarPubMed
Goldsmith, T.H. (1978 b). The effects of screening pigments on the spectral sensitivity of some Crustacea with scotopic (superposition) eyes. Vision Research 18, 475–482.CrossRefGoogle ScholarPubMed
Goldmsith, T.H., Marks, B.C. & Bernard, G.D. (1986). Separation and identification of geometric isomers of 3-hydroxyretinoids and occurrence in the eyes of insects. Vision Research 26, 1763–1769.Google Scholar
Goldsmith, T.H. & Wehner, R. (1977). Restrictions on rotational and translation diffusion of pigment in the membranes of a rhabdomeric photoreceptor. Journal of General Physiology 70, 453–490.CrossRefGoogle ScholarPubMed
Groenenduk, G.W.T., DeGrip, W.J. & Daemen, F.J.M. (1980) Quantitative determination of retinals with complete retention of their geometric configuration. Biochimica Biophysica Acta 617, 430–438Google Scholar
Hafner, G.S., Tokarski, T., Jones, C. & Martin, R. (1982). Rhabdom degradation in white-eyed and wild-type crayfish after long term dark adaptation. Journal of Comparative Physiology 148, 419–429.Google Scholar
Hamacher, K. & Stieve, H. (1984). Spectral properties of the rhodopsin-system of the crayfish Astacus leptodactylus. Photochemistry and Photobiology 39, 379–390.Google Scholar
Hariyama, T. & Tsukahara, Y. (1988). Seasonal variation of spectral sensitivity in crayfish retinular cells. Comparative Biochemistry and Physiology 91A, 529–533Google Scholar
Knowles, A. & Dartnall, H.J.A. (1977). Habitat, habit and visual pigment. In The Eye, Vol 2B, The Photobiology of Vision, 2nd edition, ed. Davson, H., pp. 581–641. New York: Academic Press.Google Scholar
Kong, K.-L. & Goldsmith, T.H. (1977). Photosensitivity of retinular cells in white-eyed crayfish (Procambarus clarkii). Journal of Comparative Physiology 122, 273–288.Google Scholar
Kito, Y., Seki, T., Suzuki, T. & Uchiyama, I. (1986). 3-Dehydroretinal in the eye of a bioluminescent squid, Watasenia scintillans. Vision Research 26, 275–279.Google Scholar
Larrivee, D. & Goldsmith, T.H. (1982). Spectral dimorphism of cray-fish visual pigment in solution. Vision Research 22, 727–737.Google Scholar
Loew, E.R. & Dartnall, H.J.A. (1976). Vitamin A1/A2-based visual pigment mixtures in cones of the rudd. Vision Research 16, 891–896.CrossRefGoogle ScholarPubMed
Makino, M., Nagai, K. & Suzuki, T. (1983). Seasonal variation of the vitamin A2-based visual pigment in the retina of adult bullfrog Rana catesbeiana. Vision Research 23, 199–204.CrossRefGoogle ScholarPubMed
Muntz, W.R.A. & Northmore, D.P.M. (1971). Visual pigments from different parts of the retina in rudd and trout. Vision Research 11, 551–561.Google Scholar
Munz, F.W. & McFarland, W.N. (1977). Evolutionary adaptations of fishes to the photic environment. In Handbook of Sensory Physiology, VII/5, The Visual System in Vertebrates, ed. Crescitelli, F., pp. 193–274. Heidelberg: Springer-Verlag.CrossRefGoogle Scholar
Nässel, D.R. (1976). The retina and retinal projection on the lamina ganglionaris of the crayfish Pacifastacus leniusculus (Dana). Journal of Comparative Neurology 167, 341–360.CrossRefGoogle Scholar
Reuter, T.E., White, R.H. & Wald, G. (1971). Rhodopsin and por-phyropsin fields in the adult bullfrog retina. Journal of General Physiology 58, 351–371.Google Scholar
Schwanzara, S.A. (1967). The visual pigments of freshwater fishes. Vision Research 7, 121–148.Google Scholar
Shaw, S.R. (1969). Optic of arthropod compound eye. Science 164, 88–90.Google Scholar
Stowe, S. (1980). Spectral sensitivity and retinal pigment movement in the crab Leptograpsus variegatus (Fabricius). Journal of Experimental Biology 87, 73–98.CrossRefGoogle ScholarPubMed
Suzuki, T. & Eguchi, E. (1987). A survey of 3-dehydroretinal as a visual pigment chromophore in various species of crayfish and other freshwater crustaceans. Experientia 43, 1111–1113.Google Scholar
Suzuki, T., Artkawa, K. & Eguchi, E. (1985). The effects of light and temperature on the rhodopsin-porphyropsin visual system of the crayfish, Procambarus clarkii. Zoological Science 2, 455–461.Google Scholar
Suzuki, T., Maeda, Y., Toh, Y. & Eguchi, E. (1988). Retinyl and 3-dehydroretinyl esters in the crayfish retina. Vision Research 28, 1061–1070.CrossRefGoogle ScholarPubMed
Suzuki, T., Makino-Tasaka, M. & Eguchi, E. (1984). 3-Dehydroretinal (vitamin A2 aldehyde) in crayfish eye. Vision Research 24, 783–787.Google Scholar
Tsin, A.T.C. & Beatty, D.D. (1980). Visual pigments and vitamins A in the adult bullfrog. Experimental Eye Research 30, 143–153.Google Scholar
Wald, G. (1967). Visual pigments of crayfish. Nature 215, 1131–1133.CrossRefGoogle ScholarPubMed
Waterman, T.H., Fernandez, H.R. & Goldsmith, T.H. (1969). Dichroism of photosensitive pigment in rhabdoms of the crayfish Or-conectes. Journal of General Physiology 54, 415–432.Google Scholar
Zeiger, J. (1992). Crayfish rhodopsin and porphyropsin: Characterization of their spectral properties, retinal localization, and in vitro chemical behavior. Ph.D. Thesis, Yale University.Google Scholar
Zeiger, J. & Goldsmith, T.H. (1989). Spectral properties of porphyropsin from an invertebrate. Vision Research 5, 519–527.Google Scholar