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Osmotic properties of Limulus seawaters and organ cultures: An unrecognized issue

Published online by Cambridge University Press:  18 February 2008

CORRINNE C.M. LIM-KESSLER ,
AMANDA R. BOLBECKER ,
JIA LI  and
GERALD S. WASSERMAN
CORRINNE C.M. LIM-KESSLER
Affiliation:
Department of Psychology, Monmouth College, Monmouth, Illinois
AMANDA R. BOLBECKER
Affiliation:
Department of Psychology and Brain Science, Indiana University, Indianapolis, Indiana
JIA LI
Affiliation:
Sensory Coding Laboratory, Department of Psychological Sciences, Purdue University, Lafayette, Indiana
GERALD S. WASSERMAN
Affiliation:
Sensory Coding Laboratory, Department of Psychological Sciences, Purdue University, Lafayette, Indiana
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Abstract

Excised eyes of the horseshoe crab (Limulus polyphemus) have long provided robust preparations for fundamental studies of the visual neuroscience of photoreceptors. Such preparations are particularly useful for immersion in perfusion chambers whose perfusate can be varied as needed. Little attention has been paid in the past to the osmotic properties of such perfusates because they have generally been virtually isotonic with natural seawater. We here report that (1) some recent Limulus studies have used organ culture perfusates that are severely hypotonic and (2) that that fact has not been adequately disclosed.

Keywords

TonicityPerfusionInfusionOrgan culture
Type
BRIEF COMMUNICATION
Information
Visual Neuroscience , Volume 25 , Issue 1 , January 2008 , pp. 103 - 105
Copyright
© 2008 Cambridge University Press

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