Hostname: page-component-cd9895bd7-gbm5v Total loading time: 0 Render date: 2024-12-25T04:20:41.906Z Has data issue: false hasContentIssue false
  • English
  • Français

Neonatal administration of the neurotoxin 5,7-dihydroxytryptamine results in synaptic reorganization in the superficial gray layer of the hamster's superior colliculus

Published online by Cambridge University Press:  02 June 2009

Erick A. Arce ,
Robert W. Rhoades  and
Richard D. Mooney
Erick A. Arce
Affiliation:
Department of Anatomy, Medical College of Ohio, Toledo
Robert W. Rhoades
Affiliation:
Department of Anatomy, Medical College of Ohio, Toledo
Richard D. Mooney
Affiliation:
Department of Anatomy, Medical College of Ohio, Toledo
Get access Rights & Permissions [Opens in a new window]

Abstract

Neonatal subcutaneous administration of the neurotoxin 5,7-dihydroxytryptamine (5,7-DHT) to hamsters results in a marked depletion of serotonin (5-HT) in cortex and an increase in the concentration of this amine in the superior colliculus (SC). To determine whether this increase was associated with an alteration in the synaptic organization of 5-HT-containing axons in the superficial gray layer of the SC, immunocytochemistry was combined with electron microscopy. In normal adult hamsters, only 4.0% of 500 5-HT-immunoreactive profiles make synaptic contacts in the superficial gray layer of the hamster's SC. In 5,7-DHT-treated animals, examination of 400 individual profiles indicated that 25.5% of 5-HT-positive profiles made synaptic contacts (P < 0.05). Given the recently demonstrated effect of 5-HT on retinotectal transmission in this species, the present results suggest that the functional organization of the SC may also be markedly altered in animals that sustain neonatal 5,7-DHT administration.

Keywords

SerotoninImmunocytochemistry5, 7-DihydroxytryptamineElectron microscopy
Type
Research Articles
Information
Visual Neuroscience , Volume 12 , Issue 4 , July 1995 , pp. 779 - 783
Copyright
Copyright © Cambridge University Press 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Arce, E.A., Bennett-Clarke, C.A., Mooney, R.D. & Rhoades, R.W. (1992). Synaptic organization of the serotoninergic input to the superficial gray layer of the hamster's superior colliculus. Synapse 11, 67–75.CrossRefGoogle Scholar
Boulenguez, P., Abdelkefi, J., Pinard, R., Christolomme, A. & Segu, L. (1993). Effects of retinal deafferentation on serotonin receptor types in the superficial grey layer of the superior colliculus of the rat. Journal of Chemical Neuroanatomy 6, 167–175.CrossRefGoogle ScholarPubMed
Chan-Palay, V. (1977). Indoleamine neurons and their processes in the normal rat brain and chronic diet-induced thiamine deficiency demonstrated by uptake of 3H-serotonin. Journal of Comparative Neurology 176, 467–494.CrossRefGoogle ScholarPubMed
Frankfurt, M. & Beaudet, A. (1987). Ultrastructural organization of regenerated serotonin axons in the dorsomedial hypothalamus of the adult rat. Journal of Neurocytology 16, 799–809.CrossRefGoogle ScholarPubMed
Fuxe, K. (1965). Evidence for the existence of monoamine neurons in the central nervous system. IV. Distribution of monoamine nerve terminals in the central nervous system. Acta Physiologica Scandinavia 64, 39–85.Google Scholar
Gerson, S. & Baldessarini, R.J. (1975). Selective destruction of serotonin terminals in rat forebrain by high doses of 5,7-dihydroxytryptamine. Brain Research 85, 140–145.CrossRefGoogle Scholar
Harvey, A.R. & Macdonald, A.M. (1987). The host serotonin projection to tectal grafts in young rats: An immunohistochemical study. Experimental Neurology 95, 688–696.CrossRefGoogle ScholarPubMed
Huang, X., Mooney, R.D. & Rhoades, R.W. (1993). Effects of serotonin (5-HT) upon retinotectal, corticotectal, and glutamate-induced activity in the superior colliculus of the hamster. Journal of Neurophysiology 70, 723–732.CrossRefGoogle ScholarPubMed
Jonsson, G., Pollare, T., Hallman, H. & Sachs, C. (1978). Developmental plasticity of central serotonin neurons after 5,7-dihydroxytryptamine treatment. Annals New York Academy of Sciences 305, 328–345.CrossRefGoogle ScholarPubMed
Kawai, N. & Yamamoto, C. (1969). Effect of 5-hydroxytryptamine, LSD, and related compounds on electrical activities evoked in vitro in thin sections from the superior colliculus. International Journal of Neuropharmacotogy 8, 437–449.Google ScholarPubMed
Mize, R.R. & Horner, L.H. (1989). Origin, distribution, and morphology of serotonergic afferents to the cat superior colliculus: A light and electron microscope immunocytochemistry study. Experimental Brain Research 75, 83–98.CrossRefGoogle Scholar
Mooney, R.D., Shi, M-Y. & Rhoades, R.W. (1994). Modulation of retinotectal transmission by presynaptic 5-HT1B receptors in the superior colliculus of the adult hamster. Journal of Neurophysiology 11, 3–13.CrossRefGoogle Scholar
Rhoades, R.W., Bennett-Clarke, C.A., Lane, R.D., Leslie, M.J. & Mooney, R.D. (1993). Increased serotoninergic innervation of the hamster's superior colliculus alters retinotectal projections. Journal of Comparative Neurology 334, 397–409.CrossRefGoogle ScholarPubMed
Sachs, C. & Jonsson, G. (1975). 5,7-Dihydroxytryptamine induced changes in the postnatal development of central 5-hydroxytryptamine neurons. Medical Biology 53, 156–164.Google ScholarPubMed
Saïdi, H. & Bosler, O. (1990). Serotonin reinnervation of the rat organum vasculosum laminae terminalis (OVLT) after 5,7-dihydroxytryptamine deafferentation. Brain Research 530, 151–155.CrossRefGoogle ScholarPubMed
Steinbusch, H.W.M. (1981). Distribution of serotonin-immunoreactivity in the central nervous system of the rat. Cell bodies and terminals. Neuroscience 6, 557–618.CrossRefGoogle ScholarPubMed
Ueda, S., Ihara, N. & Sano, Y. (1985). The organization of serotonin fibers in the mammalian superior colliculus. An immunohistochemical study. Anatomy and Embryology 173, 13–21.CrossRefGoogle ScholarPubMed
Vaughn, J.E. (1989). Review: Fine structure of synaptogenesis in the vertebrate central nervous system. Synapse 3, 255–285.CrossRefGoogle ScholarPubMed
Villar, M.J., Vitale, M.L., Hökfelt, T. & Verhofstad, A.A.J. (1988). Dorsal raphe serotoninergic branching neurons projecting both to the lateral geniculate body and superior colliculus: A combined retrograde tracing-immunohistochemical study in the rat. Journal of Comparative Neurology 277, 126–140.CrossRefGoogle Scholar
Waeber, C. & Palacios, J.M. (1990). 5-HT1, receptor binding sites in the guinea pig superior colliculus are predominantly of the 5HT1D class and are presynaptically located on primary retinal afferents. Brain Research 528, 207–211.CrossRefGoogle ScholarPubMed