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Müller cells in the retina of the cane toad, Bufo marinus, express neuropeptide Y-like immunoreactivity

Published online by Cambridge University Press:  02 June 2009

Bao-Song Zhu  and
Ian Gibbins
Bao-Song Zhu
Affiliation:
Department of Anatomy and Histology and Centre for Neuroscience, School of Medicine, The Flinders University of South Australia, Adelaide, Australia
Ian Gibbins
Affiliation:
Department of Anatomy and Histology and Centre for Neuroscience, School of Medicine, The Flinders University of South Australia, Adelaide, Australia
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Abstract

We have used light- and electron-microscopic immunohistochemistry to identify the presence of immunoreactivity to neuropeptide Y (NPY) within Müller cells in the retina of the cane toad, Bufo marinus. Müller cells containing NPY-like immunoreactivity (NPY-LI) were identified at the light-microscopic level by the coexistence with immunoreactivity to glial fibrillary acidic protein (GFAP) and at the ultrastructural level by their characteristic relationship to neuron cell bodies and processes. At the light-microscopic level, those cells which contained both NPY-LI and GFAP-LI usually had small cell bodies in the inner nuclear layer, while those cells which contained only NPY-LI were identified as large and small amacrine cells. The radially oriented primary processes in the inner plexiform layer and the vitreal end feet of GFAP-LI Müller cells also expressed NPY-LI. At the ultrastructural level, thin lamellar processes of Müller cells with NPY-LI enclosed some amacrine cell bodies in the inner nuclear layer and amacrine cell dendrites in the inner plexiform layer. These observations suggest that NPY-LI is localized in Müller cells in addition to two types of amacrine cells previously identified in the Bufo retina. This study provides the first evidence that glial elements in the vertebrate retina express NPY-LI.

Keywords

GliaMüller cellsNeuropeptide YRetinaAmphibian
Type
Research Articles
Information
Visual Neuroscience , Volume 13 , Issue 3 , May 1996 , pp. 501 - 508
Copyright
Copyright © Cambridge University Press 1996

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