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Large-scale morophological survey of rat retinal ganglion cells

Published online by Cambridge University Press:  30 October 2002

WENZHI SUN ,
NING LI  and
SHIGANG HE
WENZHI SUN
Affiliation:
Institute of Neuroscience and Shanghai Research Center for Life Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-yang Road, Shanghai 200031, P.R. China
NING LI
Affiliation:
Institute of Neuroscience and Shanghai Research Center for Life Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-yang Road, Shanghai 200031, P.R. China
SHIGANG HE
Affiliation:
Institute of Neuroscience and Shanghai Research Center for Life Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-yang Road, Shanghai 200031, P.R. China
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Abstract

Ganglion cells in an isolated wholemount preparation of the rat retina were labeled using the “DiOlistic” labeling method (Gan et al., 2000) and were classified according to their morphological properties. Tungsten particles coated with a lipophilic dye (DiI) were propelled into the wholemount retina using a gene gun. When a dye-coated particle contacted the cell membrane, the entire cell was labeled. The ganglion cells were classified into four types based on their soma size, dendritic-field size, branching pattern, and level of stratification. Broadly monostratified cells were classified into three types: RGA cells (large soma, large dendritic field); RGB cells (small- to medium-sized soma, small- to medium-sized dendritic field); and RGC cells (small- to medium-sized soma, medium-to-large dendritic field). Bistratified cells were classified as RGD. Several subtypes were identified within each ganglion cell group. A number of new subtypes were discovered and added into the existing catalog, among them were two types of bistratified cells. This study therefore represents the most complete morphological classification of rat retinal ganglion cells available to date.

Keywords

DiIMorphologyBranching patternStratification levelGene gun
Type
Research Article
Information
Visual Neuroscience , Volume 19 , Issue 4 , July 2002 , pp. 483 - 493
Copyright
2002 Cambridge University Press

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