Hostname: page-component-cd9895bd7-mkpzs Total loading time: 0 Render date: 2024-12-19T04:33:12.307Z Has data issue: false hasContentIssue false
  • English
  • Français

Inhibition of phospholipase C by U-73122 blocks one component of the receptor current in Limulus photoreceptor

Published online by Cambridge University Press:  02 June 2009

Károly Nagy  and
Klaus Contzen
Klaus Contzen
Affiliation:
Institut fur Biologie II, RWTH Aachen, Kopernikusstr. 16, D-52074 Aachen, Germany
Get access Rights & Permissions [Opens in a new window]

Abstract

In the ventral nerve photoreceptor of Limulus a short, intense flash evokes a receptor current consisting of three components. In contrast to other hypotheses, we suggested previously that only the second component of the current is activated by the phospholipase C pathway, which releases calcium from intracellular stores by inositol trisphosphate. The present paper gives further evidence to our suggestion. It is demonstrated that U-73122, a specific inhibitor for the phospholipase C, selectively blocks the second component. The first and third components are moderately affected and could still be activated after the complete block of the second one. Results support the idea that the first and third components areactivated by pathways operating independently of phospholipase C.

Keywords

Limulus photoreceptorLight-induced currentMultiple componentsPhospholipase C pathwayCurrent block
Type
Short Communication
Information
Visual Neuroscience , Volume 14 , Issue 5 , September 1997 , pp. 995 - 998
Copyright
Copyright © Cambridge University Press 1997

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bolsover, S.R. & Brown, J.E. (1985). Calcium ion, an intracellular messenger of light adaptation, also participates in excitation of Limulus photoreceptors. Journal of Physiology (London) 364, 381–393.CrossRefGoogle ScholarPubMed
Contzen, K., Richter, K-H. & Nagy, K. (1995). Selective inhibition of the phospholipase C pathway blocks one light activated current component in Limulus photoreceptor. Journal of Comparative Physiology 177, 601–610.Google Scholar
Contzen, K. & Nagy, K. (1995). Current components stimulated by different G-proteins in Limulus ventral photoreceptor. Neuroreport 6, 1905–1908.CrossRefGoogle ScholarPubMed
Contzen, K. & Nagy, K. (1996). A mathematical model for the components of the receptor current in Limulus ventral nerve photoreceptors. Journal of Photochemistry and Pholobiology, Biology 35, 123–132.CrossRefGoogle Scholar
Deckert, A., Nagy, K., Helrich, C.S. & Stieve, H. (1992). Three components in the light-induced current ofthe Limulus ventral photoreceptor. Journal of Physiology (London) 453, 69–96.CrossRefGoogle Scholar
Faddis, M.N. & Brown, J.E. (1993). Intracellular injection of heparin and polyamines. Effects on phototransduction in Limulus ventral photoreceptors. Journal of General Physiology 101, 909–931.CrossRefGoogle ScholarPubMed
Frank, T.M. & Fein, A. (1991). The role of the inositol phosphate cascade in visual excitation of invertebrate microvillar photoreceptors. Journal of General Physiology 97, 697–723.CrossRefGoogle ScholarPubMed
Hardie, R.C. & Minke, B. (1992). The trpgene is essential for a lightactivated Ca2+ channel in Drosophila photoreceptors. Neuron 8, 643–651.CrossRefGoogle Scholar
Marton, L.J. & Morris, D.R. (1987). Molecular and cellular functions of the polyamines. In Inhibition of Polyamine Metabolism, ed. Mccann, P.P., Pegg, A.E. & Sjoerdsma, A., pp. 79–105. New York: Academic Press.CrossRefGoogle Scholar
Nagy, K. (1990). Kinetic properties of single ion channels activated by light in Limulus ventral nerve photoreceptors. European Biophysics Journal 19, 47–54.Google Scholar
Nagy, K. (1991). Biophysical processes in invertebrate photoreceptors: Recent progress and a critical overview based on Limulus photoreceptors. Quarterly Reviews of Biophysics 24, 165–226.CrossRefGoogle Scholar
Nagy, K. (1993). Cyclic nucleotides and inositol trisphosphate activate distinct components of the receptor current in Limulus ventral nerve photoreceptors. Neuroscience Letters 152, 1–4.CrossRefGoogle ScholarPubMed
Nagy, K. (1994). Inhibition of the firstcomponent of the receptor current in Limulus photoreceptor. Neuroreport 5, 847–849.CrossRefGoogle Scholar
Nagy, K. & Stieve, H. (1990). Light-activated single channel currents in Limulus ventral photoreceptors. European Biophysics Journal 18, 221–224.Google Scholar
Nagy, K., Contzen, K. & Stieve, H. (1993). Two components of the receptor current are developed from distinct elementary signals in Limulus ventral nerve photoreceptor. European Biophysics Journal 22, 341–350.CrossRefGoogle Scholar
Nasi, E. (1991). Two light-dependent conductances in Lima rhabdomeric photoreceptors. Journal of General Physiology 97, 55–72.CrossRefGoogle ScholarPubMed
Nasi, E. & Gomez, M.P. (1992). Light-activated ion channels in solitary photoreceptors of the scallop Pecten irradians. Journal of General Physiology 99, 747–769.CrossRefGoogle ScholarPubMed
Payne, R. & Fein, A. (1986). The initialresponse of Limulus ventral photoreceptors to bright flashes. Released calcium as a synergist to excitation. Journal of General Physiology 87, 243–269.CrossRefGoogle Scholar
Richard, E.A., Sampta, P. &Lisman, J.E.(1995). Distinguishing between roles for calcium in Limulus photoreceptor excitation. Cell Calcium 18, 331–341.CrossRefGoogle ScholarPubMed
Schacht, J. (1978). Purification of polyphosphoinositides by chromatography on immobilized neomycin. Journal of Lipid Research 19, 1063–1067.Google Scholar
Shin, J., Richard, E.A. & Lisman, J.E. (1993). Ca2+ is an obligatory intermediate in the excitation cascade of Limulus photoreceptors. Neuron 11,845–855.CrossRefGoogle ScholarPubMed
Stieve, H. (1981). Roles of calcium in visual transduction in invertebrates. In Sense Organs, ed. Laverack, M.S., & Cosens, D.J., pp. 163–185. Glasgow and London:Blackie & Son Ltd.Google Scholar
Thompson, A.K., Mostafapour, S.P., Denlinger, L.C., Bleasdale, J.E. & Fisher, S.K. (1991). The aminosteroid U-73122 inhibits muscarinic receptor sequestration andphosphoinositide hydrolysis in SK-N-SH neuroblastoma cells. Journal of Biological Chemistry 266, 23856–23862.CrossRefGoogle ScholarPubMed
Willems, P.H.G.M., Van De Put, F.H.M.M., Engbersen, R., Bosch, R.R., Van Hof, H.J.M. & De Pont, J.J.H.H.M. (1994). Induction of Ca2+ oscillations by selective, U-73122-mediated, depletion of inositoltrisphosphate-selective Ca2+ stores in rabbit pancreatic acinar cells. Pflugers Archiv 427, 233–243.Google Scholar
Yule, D.I. & Williams, J.A.(1992). U-73122 inhibits Ca2+ oscillations in response to cholecystokinin and carbachol, but not JMV-180 in rat pancreatic acinar cells. Journal of Biological Chemistry 267, 13830–13835.CrossRefGoogle Scholar