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Horizontal cells in cat and monkey retina express different isoforms of glutamic acid decarboxylase

Published online by Cambridge University Press:  02 June 2009

Noga Vardi ,
Daniel L. Kaufman  and
Peter Sterling
Noga Vardi
Affiliation:
Department of Neuroscience, University of Pennsylvania, Philadelphia
Daniel L. Kaufman
Affiliation:
Department of Psychiatry and Biobehavioral Sciences, University of California, Los Angeles
Peter Sterling
Affiliation:
Department of Neuroscience, University of Pennsylvania, Philadelphia
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Abstract

The neurotransmitter used by horizontal cells in mammals has not been identified. GABA has been the leading candidate, but doubt has remained because of failure to clearly demonstrate the GABA synthetic enzyme, glutamic acid decarboxylase (GAD) in these cells. Because GAD was recently shown to exist as two isoforms, 65 kDa and 67 kDa, we considered whether there might be a mismatch between the forms of GAD expressed in horizontal cells and the probes used to detect it. Accordingly, we stained sections of mammalian retina with antibodies specific for each isoform. Cat horizontal cells of both types (A and B) were immunoreactive for GAD67 but negative for GAD65; monkey horizontal cells of both types (H1 and H11) were positive for GAD65 and negative for GAD67. The findings reconcile previous, apparently conflicting, observations and strengthen considerably the hypothesis that mammalian horizontal cells are GABAergic.

Keywords

RetinaCatMonkeyGABAGAD65GAD67Horizontal cellBipolar cells
Type
Research Articles
Information
Visual Neuroscience , Volume 11 , Issue 1 , January 1994 , pp. 135 - 142
Copyright
Copyright © Cambridge University Press 1994

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