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Glycine- and GABA-activated inhibitory currents on axon terminals of rabbit cone bipolar cells

Published online by Cambridge University Press:  03 February 2006

CHENGWEN ZHOU  and
RAMON F. DACHEUX
CHENGWEN ZHOU
Affiliation:
Department of Ophthalmology, University of Alabama at Birmingham, Birmingham, Alabama
RAMON F. DACHEUX
Affiliation:
Department of Ophthalmology, University of Alabama at Birmingham, Birmingham, Alabama
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Abstract

Glycine- and GABA-activated currents were examined in the axon terminals of 12 types of rabbit cone bipolar cells. In the superfused retinal slice, a cell was voltage clamped at 0 mV in the presence of cobalt; then glycine or GABA was puffed onto the axon terminal. Types CBa1, CBa2, and a few CBa1-2 cells demonstrated larger glycine-activated currents than GABA-activated ones. However, some OFF cells (CBa2n, CBa1-2n, CBa1w), most CBa1-2, and most ON cells (CBb3, CBb3-4, CBb3n, and CBb4) displayed larger GABA-activated currents. The ON cell, CBb5, possessed only a GABA-activated current. The predominance of glycinergic currents in CBa1, CBa2, and a few CBa1-2 cells suggests a major input from the glycinergic AII amacrine cell and thus a key role for these cells in the rod bipolar pathway. Certain OFF cells (most CBa1-2) expressed larger GABA-activated currents. All types expressed both GABAA and GABAC currents about equally, although most OFF types (CBa1, CB a2n, CBa1-2, and CBa2n) displayed a slightly greater GABAA component.

Keywords

Glycine-activated currentGABA-activated currentON and OFF cone bipolar cellAxon terminals
Type
Research Article
Information
Visual Neuroscience , Volume 22 , Issue 6 , November 2005 , pp. 759 - 767
Copyright
© 2005 Cambridge University Press

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