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A GAP-43-like protein in cat visual cortex

Published online by Cambridge University Press:  02 June 2009

Helen McIntosh
Affiliation:
Department of Cell Biology and Physiology, Washington University School of Medicine
David Parkinson
Affiliation:
Department of Cell Biology and Physiology, Washington University School of Medicine
Karina Meiri
Affiliation:
Department of Anatomy and Neurobiology, Washington University School of Medicine
Nigel Daw
Affiliation:
Department of Cell Biology and Physiology, Washington University School of Medicine
Mark Willard
Affiliation:
Department of Anatomy and Neurobiology, Washington University School of Medicine

Abstract

We have purified a protein that changes in relative concentration during the development of the kitten visual cortex. It resembles GAP-43 (a neuronal protein that is expressed at elevated levels during periods of development and regenerative axon growth) in the following respects: (1) it is an acidic protein (pI=4.7) whose electrophoretic mobility on SDS-PAGE is similar to, but lower than rat GAP-43, suggesting that the cat protein is larger; (2) its electrophoretic mobility varies with the acrylamide concentration in a manner that is characteristic of GAP-43; (3) its concentration in kitten forebrain is elevated during early postnatal development; (4) the sequence of ten consecutive amino acids from a chemically generated fragment matches the expected sequence from GAP-43; and (5) its amino-acid content also matches GAP-43. We conclude that our purified protein is cat GAP-43. Immunoblots with an antibody prepared against rat GAP-43 suggested that the concentration of GAP-43 in the visual cortex declines with age.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1989

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