Extracellular compartments of the blowfly eye: Ionic content and topology

Abstract

To analyze the elemental composition and topology of the extracellular compartments of the compound eye, the eyes of blowflies Calliphora vicina were rapidly frozen and ultrathin cryosections were freeze dried. Three zones of an ommatidium, peripheral cytosol of visual cells, rhabdomeres, and ommatidial cavities were analyzed by X-ray microprobe analysis. The ommatidial cavity was found to contain sodium and potassium in proportion similar to that in the blowfly hemolymph. Potassium-to-sodium ratio in a cytosol was typical for a cytosol. The rhabdomeres displayed an electrolyte content intermediate between the above compartments. Three topologically connected extracellular compartments were characterized by the experiments with tracers, monastral blue and lanthanum: (1) common intercellular space of ommatidia including peripheral clefts between the visual cells, both tracers entered this compartment; (2) the ommatidial cavity, which is not accessible for monastral blue, however, as revealed by our X-ray microanalysis, it was reachable for lanthanum; (3) rhabdomeric loops, which were accessible for lanthanum entering either via the cavity or from the common intercellular clefts. The above characteristics of the ionic content and topology of ommatidial compartments might suggest higher sodium and lower potassium content in the microvilli as compared with the cytosol. The rhabdomeric and “cavital” plasma membranes are assumed to be permeable for these ions so that a voltage of only 25–30 mV, negative inside, is probably formed across them, much lower than the known resting potential −60 mV across the peripheral plasma membrane of a visual cell.