Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-28T04:11:08.540Z Has data issue: false hasContentIssue false
  • English
  • Français

Establishing an experimental model of photodynamically induced anterior ischemic optic neuropathy

Published online by Cambridge University Press:  28 February 2011

R.S. WANG ,
P.L. LV ,
W.J. WANG ,
X.D. WANG ,
X.J. ZHANG ,
S.N. LI ,
J.Z. WANG  and
Y.J. ZENG
R.S. WANG*
Affiliation:
Department of Ophthalmology, Xi’an Fourth Hospital, Xi’an Institute of Ocular Fundus Diseases, Xi’an, Shaanxi, China
P.L. LV
Affiliation:
Department of Ophthalmology, Xi’an First Hospital, Xi’an, Shaanxi, China
W.J. WANG
Affiliation:
Department of Ophthalmology, Xi’an Fourth Hospital, Xi’an Institute of Ocular Fundus Diseases, Xi’an, Shaanxi, China
X.D. WANG
Affiliation:
Department of Ophthalmology, Affiliated Hospital of Xi’an Medical College, Xi’an, Shaanxi, China
X.J. ZHANG
Affiliation:
Beijing University of Technology, Beijing, China
S.N. LI
Affiliation:
Department of Ophthalmology, Affiliated Hospital of Xi’an Medical College, Xi’an, Shaanxi, China
J.Z. WANG
Affiliation:
Department of Ophthalmology, Xi’an Fourth Hospital, Xi’an Institute of Ocular Fundus Diseases, Xi’an, Shaanxi, China
Y.J. ZENG*
Affiliation:
Beijing University of Technology, Beijing, China
*
*Address correspondence and reprint requests to: Y.J. Zeng, Beijing University of Technology, Beijing 100022, China. E-mail: [email protected] or R.S. Wang, Department of Ophthalmology, Xi’an Fourth Hospital, Xi’an Institute of Ocular Fundus Diseases, Xi’an 710004, Shaanxi, China. E-mail: [email protected]
*Address correspondence and reprint requests to: Y.J. Zeng, Beijing University of Technology, Beijing 100022, China. E-mail: [email protected] or R.S. Wang, Department of Ophthalmology, Xi’an Fourth Hospital, Xi’an Institute of Ocular Fundus Diseases, Xi’an 710004, Shaanxi, China. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Numerous methods and drugs have been used to treat anterior ischemic optic neuropathy (AION); however, further investigations to determine the value of treatments for AION have been impeded by the lack of appropriate animal models of AION, significantly impacting on in-depth study of the disease. A rat model of AION was established, and corresponding functional changes of the fundus were observed using fundus fluorescein angiography (FFA), optical coherence tomography (OCT), and flash visual-evoked potential (F-VEP) in order to confirm the reliability of the AION model histopathologically. One day after model establishment, histopathology demonstrated that portions of the optic disc were highly edematous, with edema of nerve fibers and loose tissue, accompanied by displacement of the surrounding retina. At 23 days, the optic disc and surrounding nerve fiber layers had become thinner. None of the above-mentioned changes was observed in the laser, hematoporphyrin derivative (HPD), or naive groups. The results of fundus, FFA, F-VEP, and OCT—within 90 days after model establishment—confirmed that krypton red laser irradiation (647 nm), applied 2 h after HPD injection, can establish an ideal animal model of AION.

Keywords

AIONFFAF-VEPPhotodynamic
Type
Research Articles
Information
Visual Neuroscience , Volume 28 , Issue 2 , March 2011 , pp. 155 - 162
Copyright
Copyright © Cambridge University Press 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bernstein, S.L., Guo, Y., Kelman, S.E., Flower, R.W. & Johnson, M.A. (2003). Functional and cellular responses in a novel rodent model of anterior ischemic optic neuropathy. Investigative Ophthalmology and Visual Science 44, 4153–4162.CrossRefGoogle Scholar
Cioffi, G.A., Orgul, S., Onda, E., Bacon, D.R. & Van Buskirk, E.M. (1995). An in vivo model of chronic optic nerve ischemic: the dose-dependent effects of endothelin-I on the optic nerve vasculature. Current Eye Research 14, 1147–1153.CrossRefGoogle Scholar
Fazzone, H.E., Kupersmith, M.J. & Leibmann, J. (2003). Does topical brimonidine tartrate help NAION? British Journal of Ophthalmology 87, 119–124.Google Scholar
Goldenberg-Cohen, N., Guo, Y., Margolis, F., Cohen, Y., Miller, N.R. & Berstein, S.L. (2005). Oligodendrocyte dysfunction after induction of experimental anterior optic nerve ischemia. Investigative Ophthalmology and Visual Science 46, 2716–2725.CrossRefGoogle ScholarPubMed
Hayreh, S.S. (1974). Anterior ischaemic optic neuropathy. I. Terminology and pathogenesis. British Journal of Ophthalmology 58, 955–963.CrossRefGoogle ScholarPubMed
Johnson, E.C., Deppmeier, L.M.H., Wentzien, S.K.F., Hsu, I. & Morrison, J.C. (2000). Chronology of optic nerve head and retinal responses to elevated intraocular pressure. Investigative Ophthalmology and Visual Science 41, 431–442.Google ScholarPubMed
Knox, D.L., Kerrison, J.B. & Green, W.R. (2000). Histopathologic studies of ischemic optic neuropathy. Transactions of American Ophthalmological Society 98, 203–220.Google Scholar
Krueger-Nauq, A.M.R., Emsley, J.G., Myers, T.L., Currie, R.W. & Clarke, D.B. (2002). Injury to retinal ganglion cells induces the expression of the small heat shock protein Hsp27 in the rat visual system. Neuroscience 110, 653–665.CrossRefGoogle Scholar
Salomon, O., Dardik, R., Steinberg, D.M., Kurtz, S., Rosenberg, N., Moisseiev, J. & Huna-Baron, R. (2000). The role of angiotension converting enzyme and angiotensin II type receptor gene polymorphisms in patients with nonartertic anterior ischemic optic neuropathy. Ophthalmology 107, 1717–1720.CrossRefGoogle Scholar
Selles-Navano, I., Ellezam, S., Fajardo, R., Latour, M. & McKerracher, L. (2001). Retinal ganglion and nonneuronal cell responses in a microcrush lesion of adult rat optic nerve. Experimental Neurology 167, 282–289.CrossRefGoogle Scholar
Shinji, K., Kazuo, U., Kazunao, K., Abby, R.S. & Mitsuyoshi, N. (1998). Photochemically induced endothelial injury in the mouse as a screening model for inhibitors of vascular intimal thickening. Arteriosclerosis Thrombosis and Vascular Biology 18, 1069–1078.Google Scholar