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Effects of vasoactive intestinal peptide on ganglion cells in the rabbit retina

Published online by Cambridge University Press:  02 June 2009

Ralph J. Jensen
Ralph J. Jensen
Affiliation:
Department of Biomedical Sciences, Southern College of Optometry, Memphis
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Abstract

The effects of vasoactive intestinal peptide (VIP) on the extracellularly recorded activity of ganglion cells were studied in superfused rabbit retinas. VIP, applied to the bathing solution, significantly increased the maintained activity of both OFF-center and ON-center brisk ganglion cells. The transient, excitatory responses of these cells to flashes of light (spots or annuli centered over the receptive field) were either unaffected or moderately reduced. VIP did not affect the responses of most ON/OFF directionally selective ganglion cells to a moving light stimulus. Furthermore, maintained activity of most of these cells remained absent.

Effects of VIP on the light responses of ganglion cells were pronounced in retinas that were bathed with the dopamine D1 antagonist (+)-SCH 23390. For OFF-center brisk ganglion cells, VIP brought out both the center and surround excitatory responses that were selectively reduced by (+)-SCH 23390. Similarly, VIP brought out the leading edge responses that were reduced by (+)-SCH 23390 in ON/OFF directionally selective ganglion cells to a moving light stimulus. VIP did not however reverse the effects of (+)-SCH 23390 on ON-center brisk ganglion cells. It is argued that VIP counteracts the effects of (+)-SCH 23390 on OFF-center brisk ganglion cells and ON/OFF directionally selective ganglion cells by stimulating adenylate cyclase activity in dopamine-receptive cells of the retina.

Keywords

Vasoactive intestinal peptide (VIP)Dopamine D1 antagonistRabbit retinaGanglion cell
Type
Research Articles
Information
Visual Neuroscience , Volume 10 , Issue 1 , January 1993 , pp. 181 - 189
Copyright
Copyright © Cambridge University Press 1993

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References

Ariel, M. & Daw, N.W. (1982). Pharmacological analysis of direction-ally sensitive rabbit retinal ganglion cells. Journal of Physiology 324, 161–185.CrossRefGoogle ScholarPubMed
Brecha, N.C., Oyster, C.W. & Takahashi, E.S. (1984). Identification and characterization of tyrosine hydroxylase immunoreactive amacrine cells. Investigative Ophthalmology and Visual Science 25, 66–70.Google ScholarPubMed
Casini, G. & Brecha, N.C. (1991). Vasoactive intestinal polypeptide-containing cells in the rabbit retina: Immunohistochemical localization and quantitative analysis. Journal of Comparative Neurology 305, 313–327.CrossRefGoogle ScholarPubMed
Famiglietti, E.V. (1991). Synaptic organization of starburst amacrine cells in rabbit retina: Analysis of serial thin sections by electron microscopy and graphic reconstruction. Journal of Comparative Neurology 309, 40–70.CrossRefGoogle ScholarPubMed
Gozes, I. & Brenneman, D.E. (1989). VIP: Molecular biology and neurobiological function. Molecular Neurobiology 3, 201–236.CrossRefGoogle ScholarPubMed
Hensler, J.G. & Dubocovich, M.L. (1986). D1-dopamine receptor activation mediates [3H]acetylcholine release from rabbit retina. Brain Research 398, 407–412.CrossRefGoogle ScholarPubMed
Jensen, R.J. (1988). Vasoactive intestinal peptide reverses the effects of dopamine D-l antagonist SCH 23390 on ganglion cell activity in the rabbit retina. Investigative Ophthalmology and Visual Science (Suppl.) 29, 104.Google Scholar
Jensen, R.J. (1989). Mechanism and site of action of a dopamine D1 antagonist in the rabbit retina. Visual Neuroscience 3, 573–585.CrossRefGoogle ScholarPubMed
Jensen, R.J. (1991). Intracellular recording of light responses from visually identified ganglion cells in the rabbit retina. Journal of Neuroscience Methods 40, 101–112.CrossRefGoogle ScholarPubMed
Jensen, R.J. (1992). Effects of the dopamine antagonist (+)-SCH 23390 on intracellularly recorded responses of ganglion cells in the rabbit retina. Visual Neuroscience 8, 463–467.CrossRefGoogle ScholarPubMed
Jensen, R.J. & Daw, N.W. (1986). Effects of dopamine and its agonists and antagonists on the receptive-field properties of ganglion cells in the rabbit retina. Neuroscience 17, 837–855.CrossRefGoogle ScholarPubMed
Longshore, M.A. & Makman, M.H. (1981). Stimulation of retinal adenylate cyclase by vasoactive intestinal peptide (VIP). European Journal of Pharmacology 70, 237–240.CrossRefGoogle ScholarPubMed
Masland, R.H. (1988). Amacrine cells. Trends in Neurosciences 11, 405–410.CrossRefGoogle ScholarPubMed
Masland, R.H. & Ames, A. III (1976). Response to acetylcholine of ganglion cells in an isolated mammalian retina. Journal of Neuro-physiology 39, 1220–1235.CrossRefGoogle Scholar
Pachter, J.A. & Lam, D.M.K. (1986). Interactions between vasoactive intestinal peptide and dopamine in the rabbit retina: Stimulation of a common adenylate cyclase. Journal of Neurochemistry 46, 257–264.CrossRefGoogle ScholarPubMed
Pachter, J.A., Marshak, D.W., Lam, D.M.K. & Fry, K.R. (1989). A peptide histidine isoleucine/peptide histidine methionine-like peptide in the rabbit retina: Colocalization with vasoactive intestinal peptide, synaptic relationships, and activation of adenylate cyclase activity. Neuroscience 31, 507–519.CrossRefGoogle ScholarPubMed
Sagar, S.M. (1987). Vasoactive intestinal polypeptide (VIP) immuno-histochemistry in the rabbit retina. Brain Research 426, 157–163.CrossRefGoogle Scholar
Said, S.I. & Mutt, V. (1970). Polypeptide with broad biological activity: Isolation from small intestine. Science 169, 1217–1218.CrossRefGoogle ScholarPubMed
Schorderet, M., Hof, P. & Magistretti, P.J. (1984). The effects of VIP on cyclic AMP and glycogen levels in vertebrate retina. Peptides 5, 295–298.CrossRefGoogle ScholarPubMed
Tauchi, M., Madigan, N.K. & Masland, R.H. (1990). Shapes and distributions of the catecholamine-accumulating neurons in the rabbit retina. Journal of Comparative Neurology 293, 178–189.CrossRefGoogle ScholarPubMed
Tornqvist, K., Uddman, R., Sundler, F. & Ehinger, B. (1982). So-matostatin and VIP neurons in the retina of different species. Histochemistry 76, 137–152.CrossRefGoogle Scholar
Veruki, M.L. & Yeh, H.H. (1992). Vasoactive intestinal polypeptide modulates GABAA receptor function in bipolar cells and ganglion cells of the rat retina. Journal of Neurophysiology 67, 791–797.CrossRefGoogle ScholarPubMed