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Effects of CNQX, APB, PDA, and kynurenate on horizontal cells of the tiger salamander retina

Published online by Cambridge University Press:  02 June 2009

Xiong-Li Yang  and
Samuel M. Wu
Xiong-Li Yang
Affiliation:
Cullen Eye Institute, Baylor College of Medicine, Houston
Samuel M. Wu
Affiliation:
Cullen Eye Institute, Baylor College of Medicine, Houston
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Abstract

Effects of 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), 2-amino-4-phosphonobutyrate (APB), cis-2,3-piperidine dicarboxylic acid (PDA), and kynurenate (KYN) on the depolarizing actions of glutamate and kainate on horizontal cells (HCs) were studied in the larval tiger salamander retina. APB, PDA, and KYN hyperpolarized the HCs, but they failed to block either the actions of glutamate and kainate, or the HC light responses. APB and PDA did not cause membrane polarizations in either rods or cones, suggesting that the HC hyperpolarizations were not mediated by presynaptic actions of these compounds. CNQX, the newly synthesized non-NMDA (N-Methyl-D-Aspartate) receptor antagonist, blocked the HC light responses and the action of kainate, but not that of glutamate. These results suggest that the synaptic receptors in the tiger salamander HCs are probably non-NMDA although extra-synaptic NMDA receptors may exist in these cells.

Keywords

Retinal horizontal cells6-cyano-7-nitroquinoxaline-2,3-dione (CNQX)2-amino-4-phosphonobutyrate (APB)cis-2,3-piperidine dicarboxylic acid (PDA)Kynurenate (KYN)Glutamate receptors
Type
Research Article
Information
Visual Neuroscience , Volume 3 , Issue 3 , September 1989 , pp. 207 - 212
Copyright
Copyright © Cambridge University Press 1989

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