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Dopaminergic amacrine cells express opioid receptors in the mouse retina

Published online by Cambridge University Press:  03 April 2012

SHANNON K. GALLAGHER ,
JULIA N. ANGLEN ,
JUSTIN M. MOWER  and
JOZSEF VIGH
SHANNON K. GALLAGHER
Affiliation:
Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado
JULIA N. ANGLEN
Affiliation:
Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado
JUSTIN M. MOWER
Affiliation:
Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado
JOZSEF VIGH*
Affiliation:
Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado
*
*Address correspondence and reprint requests to: Jozsef Vigh, Department of Biomedical Sciences, Colorado State University, 1617 Campus Delivery, Fort Collins, CO 80523. E-mail: [email protected]
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Abstract

The presence of opioid receptors has been confirmed by a variety of techniques in vertebrate retinas including those of mammals; however, in most reports, the location of these receptors has been limited to retinal regions rather than specific cell types. Concurrently, our knowledge of the physiological functions of opioid signaling in the retina is based on only a handful of studies. To date, the best-documented opioid effect is the modulation of retinal dopamine release, which has been shown in a variety of vertebrate species. Nonetheless, it is not known if opioids can affect dopaminergic amacrine cells (DACs) directly, via opioid receptors expressed by DACs. This study, using immunohistochemical methods, sought to determine whether (1) μ- and δ-opioid receptors (MORs and DORs, respectively) are present in the mouse retina, and if present, (2) are they expressed by DACs. We found that MOR and DOR immunolabeling were associated with multiple cell types in the inner retina, suggesting that opioids might influence visual information processing at multiple sites within the mammalian retinal circuitry. Specifically, colabeling studies with the DAC molecular marker anti-tyrosine hydroxylase antibody showed that both MOR and DOR immunolabeling localize to DACs. These findings predict that opioids can affect DACs in the mouse retina directly, via MOR and DOR signaling, and might modulate dopamine release as reported in other mammalian and nonmammalian retinas.

Keywords

μ-opioid receptorδ-opioid receptorDopamineNeuropeptideImmunohistochemistry
Type
Brief Communication
Information
Visual Neuroscience , Volume 29 , Issue 3 , May 2012 , pp. 203 - 209
Copyright
Copyright © Cambridge University Press 2012

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