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Discrete reduction patterns of parvalbumin and calbindin D-28k immunoreactivity in the dorsal lateral geniculate nucleus and the striate cortex of adult macaque monkeys after monocular enucleation

Published online by Cambridge University Press:  02 June 2009

Ingmar Blümcke ,
Eduardo Weruaga ,
Sandor Kasas ,
Anita E. Hendrickson  and
Marco R. Celio
Ingmar Blümcke
Affiliation:
Institute of Histology and General Embryology, University of Fribourg, Rte. A. Gockel, CH-1705 Fribourg, Switzerland
Eduardo Weruaga
Affiliation:
Institute of Histology and General Embryology, University of Fribourg, Rte. A. Gockel, CH-1705 Fribourg, Switzerland
Sandor Kasas
Affiliation:
Institute of Histology and General Embryology, University of Fribourg, Rte. A. Gockel, CH-1705 Fribourg, Switzerland
Anita E. Hendrickson
Affiliation:
Department of Biological Structure and Ophthalmology, SM-20, University of Washington, Seattle
Marco R. Celio
Affiliation:
Institute of Histology and General Embryology, University of Fribourg, Rte. A. Gockel, CH-1705 Fribourg, Switzerland
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Abstract

We analyzed the immunohistochemical distribution of the two calcium-binding proteins, parvalbumin (PV) and calbindin D-28k (CB), in the primary visual cortex and lateral dorsal geniculate nucleus (dLGN) of monocularly enucleated macaque monkeys (Macaca fascicularis and Macaca nemestrind) in order to determine how the expression of PV and CB is affected by functional inactivity. The monkeys survived 1–17 weeks after monocular enucleation. The distribution pattern of each of the proteins was examined immunocytochemically using monoclonal antibodies and compared with that of the metabolic marker cytochrome oxidase (CO). We recorded manually the number of immunostained neurons and estimated the concentration of immunoreactive staining product using a computerized image-acquisition system. Our results indicate a decrease of approximately 30% in the labeling of PV-immunoreactive (ir) neuropil particularly in those layers of denervated ocular-dominance columns receiving the geniculocortical input. There was no change in the number of PV-ir neurons in any compartment irrespective of the enucleation interval. For CB-ir, we found a 20% decrease in the neuropil labeling in layer 2/3 of the denervated ocular-dominance columns. In addition, a subset of pyramidal CB-ir neurons in layers 2 and 4B, which are weakly stained in control animals, showed decreased labeling. In the dLGN of enucleated animals, PV-ir and CB-ir were decreased only in the neuropil of the denervated layers.

From these results, we conclude that cortical interneurons and geniculate projection neurons still express PV and CB in their cell bodies after disruption of the direct functional input from one eye. The only distinct decrease of PV and CB expression is seen in axon terminals from retinal ganglion cells in the dLGN, and in the axons and terminals of both geniculocortical projection cells and cortical interneurons in the cerebral cortex.

Keywords

Calcium-binding proteinsCytochrome oxidaseVisual deprivationNeuroanatomyPrimates
Type
Research Articles
Information
Visual Neuroscience , Volume 11 , Issue 1 , January 1994 , pp. 1 - 11
Copyright
Copyright © Cambridge University Press 1994

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