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Classification of potassium and chlorine ionic currents in retinal ganglion cell line (RGC-5) by whole-cell patch clamp

Published online by Cambridge University Press:  30 October 2012

SHU-JIE WANG ,
LAI-HUA XIE ,
BIN HENG  and
YAN-QIANG LIU
SHU-JIE WANG
Affiliation:
Department of Zoology and Developmental Biology, College of Life Science, Nankai University, Tianjin, People’s Republic of China
LAI-HUA XIE
Affiliation:
Department of Cell Biology and Molecular Medicine, UMDNJ-New Jersey Medical School, Newark, New Jersey
BIN HENG
Affiliation:
Department of Zoology and Developmental Biology, College of Life Science, Nankai University, Tianjin, People’s Republic of China
YAN-QIANG LIU*
Affiliation:
Department of Zoology and Developmental Biology, College of Life Science, Nankai University, Tianjin, People’s Republic of China
*
*Address correspondence and reprint requests to: Dr. Yanqiang Liu, College of Life Sciences, Nankai University, Tianjin 300071, People’s Republic of China. E-mail: [email protected], [email protected]
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Abstract

Retinal ganglion cell line (RGC-5) has been widely used as a valuable model for studying pathophysiology and physiology of retinal ganglion cells in vitro. However, the electrophysiological characteristics, especially a thorough classification of ionic currents in the cell line, remain to be elucidated in details. In the present study, we determined the resting membrane potential (RMP) in RGC-5 cell line and then identified different types of ionic currents by using the whole-cell patch-clamp technique. The RMP recorded in the cell line was between −30 and −6 mV (−17.6 ± 2.6 mV, n = 10). We observed the following voltage-gated ion channel currents: (1) inwardly rectifying Cl− current (ICl,ir), which could be blocked by Zn2+; (2) Ca2+-activated Cl− current (ICl,Ca), which was sensitive to extracellular Ca2+ and could be inhibited by disodium 4,4’-diisothiocyanatostilbene-2,2’-disulfonate; (3) inwardly rectifying K+ currents (IK1), which could be blocked by Ba2+; (4) a small amount of delayed rectifier K+ current (IK). On the other hand, the voltage-gated sodium channels current (INa) and transient outward potassium channels current (IA) were not observed in this cell line. These results further characterize the ionic currents in the RGC-5 cell line and are beneficial for future studies especially on ion channel (patho)physiology and pharmacology in the RGC-5 cell line.

Keywords

Retinal ganglion cell lineElectrophysiological characteristicsWhole-cell patch-clamp technique
Type
Review Articles
Information
Visual Neuroscience , Volume 29 , Issue 6 , November 2012 , pp. 275 - 282
Copyright
Copyright © Cambridge University Press 2012

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