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Changing topography of the RPE resulting from experimentally induced rapid eye growth

Published online by Cambridge University Press:  02 June 2009

Patricia A. Fleming ,
Alison M. Harman  and
Lyn D. Beazley
Patricia A. Fleming
Affiliation:
Department of Psychology, The University of Western Australia, Nedlands, Western Australia6009
Alison M. Harman
Affiliation:
Department of Psychology, The University of Western Australia, Nedlands, Western Australia6009
Lyn D. Beazley
Affiliation:
Deparlment of Zoology, The University of Western Australia, Nedlands, Western Australia6009
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Abstract

The retinal pigment epithelium (RPE) of the quokka wallaby, Setonix brachyurus, grows and changes throughout life. To investigate factors that determine changes in the quokka RPE, we have examined topography of this tissue in experimentally enlarged eyes. Unilateral eyelid suture was conducted at the time of normal eye opening, postnatal day (P) 110, and animals were examined at 1 or 1½ years of age. The numbers and densities of RPE cells and the extent of multinucleation were compared with those in normal animals. Eyelid suture resulted in a 9.8% and 17.4% increase in retinal area at 1 and 1½ years, respectively; a significant degree of myopia was associated with this enlargement. Cell density topography in experimental eyes was not the same as in controls. Cells from central retina were disproportionately larger in the experimental than control eyes. However, the RPE cell topography in sutured eyes was not the same as that of aged retinae of a similar size. Notably, in sutured eyes there was no development of the high or highest cell densities seen in equatorial and temporal central RPE in aged retinae, respectively. Furthermore, the degree of cell enlargement in peripheral regions was slight compared with that observed in similar-sized, aged retinae. There was no increase in RPE cell number; rather, average cell area increased accompanied by no change or a slight decrease in RPE thickness. Consequently, overall volume of cells did not change significantly. The large number of multinucleate cells normally seen in aged animals was not observed in experimentally enlarged eyes, implying that an increase in cell volume may be the trigger for multinucleation.

Keywords

AgingCell sizeMammalMultinucleateRetinal growthDevelopment
Type
Research Articles
Information
Visual Neuroscience , Volume 14 , Issue 3 , May 1997 , pp. 449 - 461
Copyright
Copyright © Cambridge University Press 1997

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