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Bipolar cells of the chick retina containing α-bungarotoxin-sensitive nicotinic acetylcholine receptors

Published online by Cambridge University Press:  02 June 2009

Dânia E. Hamassaki-Britto ,
Agnieszka Brzozowska-Prechtl ,
Harvey J. Karten  and
Jon M. Lindstrom
Dânia E. Hamassaki-Britto
Affiliation:
Department of Neurosciences, University of California San Diego, La Jolla Department of Histology and Embryology/ICB, University of São Paulo, São Paulo, SP, Brazil
Agnieszka Brzozowska-Prechtl
Affiliation:
Department of Neurosciences, University of California San Diego, La Jolla
Harvey J. Karten
Affiliation:
Department of Neurosciences, University of California San Diego, La Jolla
Jon M. Lindstrom
Affiliation:
Institute of Neurological Sciences, University of Pennsylvania, Philadelphia
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Abstract

Two cDNA clones for nicotinic acetylcholine receptor (nAChR) subunits sensitive to α-bungarotoxin (α-Bgt) have been isolated, the so-called α-Bgt binding proteins α1 (or α7 nAChR subunit) and α2 (or α8 nAChR subunit). Immunohistochemical experiments have shown that both α7 and α8 subunits, as well as subunits insensitive to α-Bgt (β2 and α3), are present in amacrine and ganglion cells of the chick retina. However, only the α8 subunit was observed in presumptive bipolar cells. The present study investigated in detail the pattern of distribution of the bipolar cells containing the α8 nAChR subunit and its relation to the pattern of distribution of bipolar cells immunoreactive to protein kinase C (PKC). Presumptive α8-and PKC-like immunoreactive (α8-LI and PKC-LI) bipolar cells were observed sending their dendrites to the outer plexiform layers and their axons to the inner plexiform layer. Where as α8-LI bipolar cells corresponded to 40–53% of the whole population of bipolar cells, PKC-LI bipolar cells represented only 6–8% of the same population. The soma sizes of the α8-LI bipolar cells were slightly smaller (mean ± s.d.; 4.9 ± 0.8 μm) than the soma sizes of the PKC-LI bipolar cells (5.4 ± 0.9 μm). Double-labeling experiments indicated that probably all PKC-LI bipolar cells also contain α8-LI. This indicates that two distinct groups of cholinoceptive bipolar cells exist in the chick retina, one that contains PKC-LI, and another one that does not.

Keywords

Bipolar cellsNicotinic receptorsProtein kinase C.α-BgtRetina
Type
Research Articles
Information
Visual Neuroscience , Volume 11 , Issue 1 , January 1994 , pp. 63 - 70
Copyright
Copyright © Cambridge University Press 1994

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