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Wavelength-dependent temporal properties of retinal horizontal cells in turtles

Published online by Cambridge University Press:  02 June 2009

C.A. Dvorak  and
A.M. Granda
C.A. Dvorak
Affiliation:
Program in Neurosciences, School of Life Sciences, University of Delawarer, Newark
A.M. Granda
Affiliation:
Program in Neurosciences, School of Life Sciences, University of Delawarer, Newark
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Abstract

Electrical reponses of luminosity horizontal cells (L cells) to monochromatic stimuli were analyzed by intracellular recordings in the retinas of the freshwater turtle (Pseudemys scripta elegans) and of the sea turtle (Chelonia mydas mydas). Light intensity, duration, and wavelength were varied to assess temporal effects. For a given intensity of monochromatic light, response amplitude increased with stimulus duration until maximum amplitude occurred at a specific duration. This suprathreshold metric of temporal integration is called here summation time, and it is wavelength-dependent.

L cells always had some level of red-sensitive cone input, although in some cells inputs from green- and blue-sensitive cones were also observed. For these latter cells, summation times were shorter for 640-nm than for 540-nm or 450-nm lights. These results were most evident in cells that received dominant inputs from blue- or green-sensitive cones.

Responses of some other L cells were almost completely dominated by inputs from red-sensitive cones. Summation times of these cells were not wavelength-dependent. But when these inputs also included green-sensitive cones, shorter summation times were obtained to 640-nm light than to 540-nm light, even though dominant inputs were still from red-sensitive cones. These results, obtained from both retinal and 3,4-dehydroretinal photopigment systems, are consistent with reported observations in Pseudemys scripta elegans that show linear responses of red-sensitive cones to have shorter integration times and times-to-peak than green-sensitive cones.

Responses from horizontal cells dominated by blue-sensitive cone inputs were the most sensitive of all; they also had the longest summation times. These results support the hypothesis that a gain in sensitivity occurs from the integration of absorbed photons over longer periods of time.

These intracellular responses are of particular importance because behavioral critical durations in turtle, as defined by Bloch&'s law, are similarly wavelength-dependent.

Keywords

Wavelenght dependenceTemporal propertiesHorizontal cellTurtle vision
Type
Research Articles
Information
Visual Neuroscience , Volume 4 , Issue 5 , May 1990 , pp. 427 - 435
Copyright
Copyright © Cambridge University Press 1990

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