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Visual behavior of adult goldfish with regenerating retina

Published online by Cambridge University Press:  26 June 2007

AMY E. LINDSEY  and
MAUREEN K. POWERS
AMY E. LINDSEY
Affiliation:
Department of Psychology and Vanderbilt Vision Research Center, Vanderbilt University, Nashville, Tennessee
MAUREEN K. POWERS
Affiliation:
Department of Psychology and Vanderbilt Vision Research Center, Vanderbilt University, Nashville, Tennessee
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Abstract

To determine whether regenerating neural pathways can support visual behavior, adult goldfish (Carassius auratus) were injected intraocularly with ouabain and tested for the presence of reflexive visual behaviors (dorsal light reflex and optokinetic nystagmus) and the ability to respond to visual stimuli in a classical conditioning paradigm. All visual behaviors were absent or greatly diminished until 8 to 10 weeks, when retinal layering had returned. At 10 weeks post-ouabain, reflexive behaviors to supra-threshold stimuli were near normal; however the ability to detect supra-threshold stimuli in the conditioning paradigm did not recover until 13 weeks. Absolute dark-adapted threshold and light-adapted spectral sensitivity measured at 13 to 17 weeks were abnormal: Dark-adapted threshold was elevated by 1.5 log units and light-adapted spectral sensitivity was markedly narrower than normal. No responses to 50% contrast sinusoidal gratings could be obtained through ouabain-treated eyes using the classical conditioning technique, even though responses through the untreated eye remained. Results demonstrate that: (a) visually mediated behaviors return in goldfish with ouabain-treated retinas; (b) the time course of recovery of reflexive responses in luminance and spatial domains parallels return of ERG function and of tectal activity; and (c) visual function that is mediated by regenerating retina appears not to be as sensitive as vision via normally developed retinal pathways.

Keywords

GoldfishOKNLight reflexBehaviorRetinaRegeneration
Type
Research Article
Information
Visual Neuroscience , Volume 24 , Issue 3 , May 2007 , pp. 247 - 255
Copyright
© 2007 Cambridge University Press

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