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Serial inhibitory synapses in retina

Published online by Cambridge University Press:  02 June 2009

Jian Zhang ,
Chang-Sub Jung  and
Malcolm M. Slaughter
Jian Zhang
Affiliation:
Departments of Physiology, Biophysical Sciences, and Ophthalmology, School of Medicine, State University of New York, Buffalo
Chang-Sub Jung
Affiliation:
Departments of Physiology, Biophysical Sciences, and Ophthalmology, School of Medicine, State University of New York, Buffalo
Malcolm M. Slaughter
Affiliation:
Departments of Physiology, Biophysical Sciences, and Ophthalmology, School of Medicine, State University of New York, Buffalo
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Abstract

Whole-cell voltage clamp in the retinal slice and intracellular current clamp in the intact retina were used to study inhibitory interactions in the inner plexiform layer. Picrotoxin or strychnine reduced inhibitory, light-evoked currents in a majority of ganglion cells. However, in nearly a third of the ganglion cells, each of these antagonists enhanced the inhibitory synaptic current. All inhibitory current was blocked by the addition of the other antagonist. This indicates a cross-inhibition between GABAergic and glycinergic feedforward pathways. Blocking of GABAARs with SR95531 shortened the time course of both excitatory and inhibitory synaptic currents in ganglion cells. Application of picrotoxin, which blocked both GABAARs and GABACRs, produced the opposite effect. Recordings in the intact retina indicated that the light responses of ON bipolar cells, sustained ON, and transient ON-OFF third-order neurons were all made more transient by SR95531 and made more sustained by picrotoxin. The data suggest that a GABAC feedback pathway to bipolar cells makes light responses more phasic and that this feedback is inhibited through a GABAAR pathway. Consequently, the balance between GABAAR and GABACR inhibition regulates the time course of inputs to ganglion cells.

Keywords

RetinaGABAGlycineGanglion cellsInhibition
Type
Research Articles
Information
Visual Neuroscience , Volume 14 , Issue 3 , May 1997 , pp. 553 - 563
Copyright
Copyright © Cambridge University Press 1997

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