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Packaging of rhodopsin and porphyropsin in the compound eye of the crayfish

Published online by Cambridge University Press:  02 June 2009

Joel Zeiger  and
Timothy H. Goldsmith
Joel Zeiger
Affiliation:
Department of Biology, Yale University, New Haven
Timothy H. Goldsmith
Affiliation:
Department of Biology, Yale University, New Haven
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Abstract

The distribution of 3-dehydroretinal (Ral2) in dorsal, middle, and ventral slices of eyes of the crayfish Procambarus clarkii was examined by HPLC. No pronounced differences were found. Similar results were obtained when the eyes were cut into anterior, intermediate, and posterior portions.

Dichroic difference spectra were measured in single halves of microvillar layers of isolated rhabdoms and the proportions of rhodopsin (P1) and porphyropsin (P2) estimated by comparison with computer-generated mixtures of these pigments, whose spectra are known from previous work. The fraction of visual pigment that is porphyropsin appears to be uniform throughout individual retinular cells and among the retinular cells of individual rhabdoms, but various substantially among different rhabdoms from the same eye.

The interommatidial variation in the amount of P2 greatly exceeds the gross regional variation in Ral2. This means there is an intermingling of ommatidia with different levels of P2. The variability in P2 among ommatidia is not likely to have important implications for the vision of the crayfish but suggests that in the metabolism of retinoids, individual ommatidia are quasi-independent metabolic units. The results are compatible with a single opsin for both crayfish rhodopsin and porphyropsin.

Keywords

Porphyropsin3-DehydroretinalCrayfishMicrospectrophotometry
Type
Research Article
Information
Visual Neuroscience , Volume 10 , Issue 2 , March 1993 , pp. 193 - 202
Copyright
Copyright © Cambridge University Press 1993

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