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Immunocytochemical localization of dopamine D1 receptors in the retina of mammals

Published online by Cambridge University Press:  02 June 2009

J. Nguyen-Legros ,
A. Simon ,
I. Caillé  and
B. Bloch
J. Nguyen-Legros
Affiliation:
INSERM U-86, Laboratoire de NeuroCytologie Oculaire, Paris Cedex, France
A. Simon
Affiliation:
INSERM U-86, Laboratoire de NeuroCytologie Oculaire, Paris Cedex, France
I. Caillé
Affiliation:
EP CNRS 74, Laboratoire d'histologie-Embryologie (UFR II), Université de Bordeaux II, Bordeaux Cedex, France
B. Bloch
Affiliation:
EP CNRS 74, Laboratoire d'histologie-Embryologie (UFR II), Université de Bordeaux II, Bordeaux Cedex, France
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Abstract

Dopamine is one of the major neurotransmitters in the retina. It is released from amacrine and interplexiform cells into both inner (IPL) and outer (OPL) plexiform layers. Several dopaminergic actions are known to occur through D1 receptors (D1R) but the precise location of these receptors has not been established. An antibody that recognizes the intracytoplasmic C-terminal of the rat D1R was used to detect D1R, immunohistochemically, in rats (Wistar and RCS), mouse, hamster, and macaque monkey retinas. The OPL was heavily stained in each species, consistent with the known actions of dopamine on horizontal cells. Three to five bands were observed in the IPL, depending on species. Three were in the a sublayer, the outermost of which was close to the amacrine cell layer, and may represent the massive dopamine input to the AII rod-amacrine cells. As observed in mice, where bipolar cells are D1-immunoreactive, the band located in sublayer 3 of the IPL may contain cone-bipolar cell terminals. A band of D1R-immunoreactivity in the b sublayer of the IPL contains ON-bipolar cell terminals and a second site of interaction between dopaminergic cells and the AII amacrine cells. This sublayer was absent from the RCS rat retina, suggesting a severe impairment of the rod-driven pathway following rod degeneration in these mutant rats. Cells in the ganglion cell layer exhibited relatively heavy staining, and may be ganglion cells or displaced amacrine cells. Some extrasynaptic localizations of D1R in the retina are suggested.

Keywords

SynapsePrimate foveaRCS ratsBipolar cells
Type
Research Articles
Information
Visual Neuroscience , Volume 14 , Issue 3 , May 1997 , pp. 545 - 551
Copyright
Copyright © Cambridge University Press 1997

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