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Genotyping Accuracy for Whole-Genome Amplification of DNA from Buccal Epithelial Cells

Published online by Cambridge University Press:  21 February 2012

Keren Leviel ,
Megan Olarte  and
Patrick F. Sullivan
Keren Leviel
Affiliation:
Department of Genetics, University of North Carolina at Chapel Hill, United States of America.
Megan Olarte
Affiliation:
Department of Genetics, University of North Carolina at Chapel Hill, United States of America.
Patrick F. Sullivan*
Affiliation:
Department of Genetics, University of North Carolina at Chapel Hill, United States of America. [email protected]
*
*Address for correspondence: Dr Sullivan, Department of Genetics, CB#7264, 4109 Neurosciences Research Building, University of North Carolina, Chapel Hill, NC, 27599-7264, USA.

Abstract

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We compared the accuracy of genotyping for DNA extracted from lymphocytes to that of DNA amplified from buccal epithelial cells. Amplification was via a rolling circle/φ29 DNA polymerase commercial kit. Paired buccal and lymphocyte DNA samples were available from 30 individuals. All samples were genotyped for 12 SNPs, 5 microsatellites and 2 VNTRs. The accuracy of genotyping (no-call proportions, reproducibility, and concordance) was similar for DNA from lymphocytes in comparison to amplified DNA from buccal samples. If used with caution, these data suggest that rolling-circle whole-genome amplification can be used to increase the DNA mass available for large-scale genotyping projects based on DNA from buccal cells.

Type
Articles
Information
Twin Research and Human Genetics , Volume 7 , Issue 5 , 01 October 2004 , pp. 482 - 484
Copyright
Copyright © Cambridge University Press 2004