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Threshold temperatures for seed germination in nine species of Verbascum (Scrophulariaceae)

Published online by Cambridge University Press:  08 December 2015

Stefania Catara ,
Antonia Cristaudo ,
Andrea Gualtieri ,
Rosario Galesi ,
Carmen Impelluso  and
Andrea Onofri
Stefania Catara
Affiliation:
Department of Biological, Geological and Environmental Sciences, University of Catania, Via Empedocle 58, 95128 Catania, Italy
Antonia Cristaudo*
Affiliation:
Department of Biological, Geological and Environmental Sciences, University of Catania, Via Empedocle 58, 95128 Catania, Italy
Andrea Gualtieri
Affiliation:
Department of Biological, Geological and Environmental Sciences, University of Catania, Via Empedocle 58, 95128 Catania, Italy
Rosario Galesi
Affiliation:
Department of Biological, Geological and Environmental Sciences, University of Catania, Via Empedocle 58, 95128 Catania, Italy
Carmen Impelluso
Affiliation:
Department of Biological, Geological and Environmental Sciences, University of Catania, Via Empedocle 58, 95128 Catania, Italy
Andrea Onofri
Affiliation:
Department of Agricultural, Food and Environmental Sciences, University of Perugia, Borgo XX Giugno 74, 06121 Perugia, Italy
*
*Correspondence E-mail: [email protected]
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Abstract

Light and fluctuating temperatures are two important factors triggering seed germination. The aim of this work was to: (1) elucidate the effect of temperature, light regime and storage time on seed germination in nine taxa of Verbascum spp., collected from different habitats in the Mediterranean area; and (2) estimate threshold temperatures for seed germination. For all taxa, germination assays were performed at constant and fluctuating temperatures, both in continuous darkness (D) and in alternating light/dark (L/D; 12 h photoperiod). Final germinated proportions (FGPs), base (T b), optimal (T o) and cut-off (T c) temperatures were derived. At constant temperatures, seed germination was strongly suppressed under the D regime. In L/D, the effect of storage time was very small and the highest FGPs were observed from 15 to 30°C (40–100%), depending on the species. T b ranged from below 7 to above 10°C and it appeared to be constant within each seed lot. T o and T c showed some within-lot variability and were higher for fast-germinating seeds in each lot. Considering fluctuating temperatures, germination appeared to be quicker and more complete than at constant temperatures. The germination of Verbascum spp. is favoured in L/D and fluctuating temperatures, which explains their pioneer character when positioned near the soil surface and under a low vegetation canopy. V. arcturus and V. pinnatifidum were shown to be less favoured by L and fluctuating temperatures, which might explain their ability to germinate in rocky areas or sandy dunes, even when they are not directly exposed to the light.

Keywords

cardinal temperaturesconstant and alternating temperatureslight regimeseed germinationstorage timethermal-timeVerbascum plant species
Type
Research Papers
Information
Seed Science Research , Volume 26 , Issue 1 , March 2016 , pp. 30 - 46
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Copyright
Copyright © Cambridge University Press 2015 

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References

Balisky, A.C. and Burton, P.J. (1993) Distinction of soil thermal regimes under various experimental vegetation covers. Canadian Journal of Soil Science 73, 411–420.Google Scholar
Ballaré, C.L. (1994) Light gaps: sensing the light opportunities in highly dynamic canopy environments. pp. 73–110 in Caldwell, M.M.; Pearcy, R.W. (Eds) Exploitation of environmental heterogeneity by plants: Ecophysiological processes above and below ground. New York, Academic Press.CrossRefGoogle Scholar
Baskin, C.C. and Baskin, J.M. (2014) Seeds: Ecology, biogeography, and evolution of dormancy and germination (2nd edition). London, Academic Press.Google Scholar
Baskin, J.M. and Baskin, C.C. (1981) Seasonal changes in germination responses of buried seeds of Verbascum thapsus and V. blattaria and ecological implications. Canadian Journal of Botany 59, 1769–1775.Google Scholar
Benedí, C. (2009) Verbascum L. pp. 49–97 in Castroviejo, S.; Aedo, C.; Laínz, M.; Muñoz Garmendia, F.; Nieto Feliner, G.; Paiva, J.; Benedí, C. (Eds) Flora iberica. Vol.13. Madrid, Real Jardín Botánico, CSIC.Google Scholar
Bolker, B. (2008) Ecological models and data in R. Princeton, Princeton University Press.Google Scholar
Bradford, K.J. (1995) Water relations in seed germination. pp. 351–396 in Kigel, J.; Galili, G. (Eds) Seed development and germination. New York, Marcel Dekker.Google Scholar
Bradford, K.J. (2005) Threshold models applied to seed germination ecology. New Phytologist 165, 338–341.Google Scholar
Chantre, G.R., Batlla, D., Sabbatini, M.R. and Orioli, G. (2009) Germination parameterization and development of an after-ripening thermal-time model for primary dormancy release of Lithospermum arvense seeds. Annals of Botany 103, 1291–1301.Google Scholar
Cochrane, A., Yates, C.J., Hoyle, G.L. and Nicotra, A.B. (2014) Will among-population variation in seed traits improve the chance of species persistence under climate change? Global Ecology and Biogeography 24, 12–24.Google Scholar
Conti, E., Abbate, G., Alessandrini, A. and Blasi, C. (2005) An annotated checklist of the Italian vascular flora. Roma, Palombi Ed.Google Scholar
Covell, S., Ellis, R.H., Roberts, E.H. and Summerfield, R.J. (1986) The influence of temperature on seed germination rate in grain legumes. I. A comparison of chickpea, lentil, soyabean and cowpea at constant temperatures. Journal of Experimental Botany 37, 705–715.CrossRefGoogle Scholar
Cristaudo, A., Gresta, F., Catara, S. and Mingo, A. (2014a) Assessment of daily heat pulse regimes on the germination of six Amaranthus species. Weed Research 54, 366–376.Google Scholar
Cristaudo, A., Gresta, F., Restuccia, A., Catara, S. and Onofri, A. (2014b) Germinative response of redroot pigweed (Amaranthus retroflexus L.) to environmental conditions: Is there a seasonal pattern? Plant Biosystems. doi:10.1080/11263504.2014.987845.Google Scholar
Darlington, H.T. and Steinbauer, G.P. (1961) The eighty-year period for Dr. Beal's seed viability experiment. American Journal of Botany 48, 321–325.Google Scholar
Demel, T. (1996) Germination ecology of twelve indigenous and eight exotic multipurpose leguminous species from Ethiopia. Forest Ecology and Management 80, 209–223.Google Scholar
Demel, T. (2005) Seed and regeneration ecology in dry Afromontane forests of Ethiopia: I. Seed production – population structures. Tropical Ecology 46, 29–44.Google Scholar
Demel, T. and Granström, A. (1997) Seed viability of Afromontane tree species in forest soils. Journal of Tropical Ecology 13, 81–95.Google Scholar
Doussi, M.A. and Thanos, C.A. (1997) Ecophysiology of seed germination in composites inhabiting fire-prone Mediterranean ecosystems. pp. 641–649 in Ellis, R.H.; Black, M.; Murdoch, A.J.; Hong, T.D. (Eds) Basic and applied aspects of seed biology: Proceedings of the Fifth International Workshop on Seeds, Reading, 1995. Dordrecht, Kluwer Academic.CrossRefGoogle Scholar
Ferguson, I.K. (1972) Verbascum L. pp. 205–216 in Tutin, T.G.; Burges, N.A.; Chater, A.O.; Edmondson, J.R.; Heywood, V.H.; Moore, D.M.; Valentine, D.H.; Walters, S.M.; Webb, D.A. (Eds) Flora Europaea. Vol. 3. Diapensiaceae to Myoporaceae. Cambridge, Cambridge University Press.Google Scholar
Finch-Savage, W.E., Steckel, J.R.A. and Phelps, K. (1998) Germination and post-germination growth to carrot seedling emergence: predictive threshold models and sources of variation between sowing occasions. New Phytologist 139, 505–516.Google Scholar
Garcia-Huidobro, J., Monteith, J. and Squire, R. (1982) Time, temperature and germination of pearl millet (Pennisetum typhoides S. & H.). 2. Alternating temperatures. Journal of Experimental Botany 33, 297–300.Google Scholar
Gardarin, A., Guillemin, J.-P., Munier-Jolain, N.M. and Colbach, N. (2010) Estimation of key parameters for weed population dynamics models: base temperature and base water potential for germination. European Journal of Agronomy 32, 162–168.Google Scholar
Gardner, W.A. (1921) Effect of light on germination of light-sensitive seeds. Botanical Gazette 71, 249–288.CrossRefGoogle Scholar
Goggin, D.A. and Steadman, K.J. (2012) Blue and green are frequently seen: responses of seeds to short- and mid-wavelength light. Seed Science Research 12, 27–35.Google Scholar
Grime, J.P., Mason, G., Curtis, A.V., Rodman, J., Band, S.R., Mowforth, M.A.G., Neal, A.M. and Shaw, S. (1981) A comparative study of germination characteristics in a local flora. Journal of Ecology 69, 1017–1059.Google Scholar
Gross, K.L. (1985) Effects of irradiance and spectral quality on the germination of Verbascum thapsus L. and Oenothera biennis L. seeds. New Phytologist 101, 531–541.Google Scholar
Gross, K.L. and Werner, P.A. (1978) The biology of Canadian weeds. 28. Verbascum thapsus L. and V. blattaria L. Canadian Journal of Plant Science 58, 401–413.CrossRefGoogle Scholar
Gross, K.L. and Werner, P.A. (1982) Colonizing abilities of ‘biennial’ plant species in relation to ground cover: implications for their distributions in a successional sere. Ecology 63, 921–931.Google Scholar
Hoshovsky, M.C. (1986) Element stewardship abstract for Verbascum thapsus (common mullein). Arlington, Virginia, The Nature Conservancy.Google Scholar
Judd, W.S., Campbell, C.S., Kellogg, E.A., Stevens, P.F. and Donoghue, M.J. (2008) Plant systematics: A phylogenetic approach. Sunderland, Massachusetts, Sinauer.Google Scholar
Kivilaan, A. and Bandurski, R.S. (1981) The one hundred-year period for Dr. Beal's seed viability experiment. American Journal of Botany 68, 1290–1292.CrossRefGoogle Scholar
Koutsovoulou, K., Daws, M.I. and Thanos, C.A. (2014) Campanulaceae: a family with small seeds that require light for germination. Annals of Botany 113, 135–143.CrossRefGoogle ScholarPubMed
Legendre, P. and Legendre, L. (1998) Numerical ecology. Amsterdam, Elsevier Science.Google Scholar
Law, C.G. and Brookmeyer, R. (1992) Effects of mid-point imputation on the analysis of doubly censored data. Statistics in Medicine 11, 1569–1578.CrossRefGoogle ScholarPubMed
Luna, B., Pérez, B., Fernández-González, F. and Moreno, J.M. (2004) Sensitivity to green safelight of 12 Mediterranean species. Seed Science and Technology 32, 113–117.Google Scholar
Maruta, E. (1983) Growth and survival of current-year seedlings of Polygonum cuspidatum at the upper distribution limit on Mt. Fuji. Oecologia 60, 316–320.Google Scholar
Maruta, E. (1994) Seedling establishment of Polygonum cuspidatum and Polygonum weyrichii var. alpinum at high altitudes of Mt. Fuji. Ecological Research 9, 205–213.Google Scholar
McNair, J.N., Sunkara, A. and Frobish, D. (2012) How to analyse seed germination data using statistical time-to-event analysis: non-parametric and semi-parametric methods. Seed Science Research 22, 77–95.Google Scholar
Mesgaran, M.B., Mashhadi, H.R., Alizadeh, H., Hunt, J., Young, K.R. and Cousens, R.D. (2013) Importance of distribution function selection for hydrothermal time models of seed germination. Weed Research 53, 89–101.Google Scholar
Milberg, P., Andersson, L. and Thompson, K. (2000) Large-seeded species are less dependent on light for germination than small-seeded ones. Seed Science Research 10, 99–104.Google Scholar
Onofri, A., Gresta, F. and Tei, F. (2010a) A new method for the analysis of germination and emergence data of weed species. Weed Research 50, 187–198.Google Scholar
Onofri, A., Carbonell, E., Piepho, H.P., Mortimer, A.M. and Cousens, R.D. (2010b) Current statistical issues in weed research. Weed Research 50, 5–24.CrossRefGoogle Scholar
Onofri, A., Mesgaran, M., Neve, P. and Cousens, R. (2014) Experimental design and parameter estimation for threshold models in seed germination. Weed Research 54, 425–435.Google Scholar
Parker, I.M., Rodriguez, J. and Michael, E.L. (2003) An evolutionary approach to understanding the biology of invasions: local adaptation and general-purpose genotypes in the weed Verbascum thapsus . Conservation Biology 17, 59–72.CrossRefGoogle Scholar
Parmoon, G., Moosavi, S., Akbari, A.H. and Ebadi, A. (2015) Quantifying cardinal temperatures and thermal time required for germination of Silybum marianum seed. The Crop Journal 3, 145–151.Google Scholar
Pignatti, S. (1982) Flora d'Italia, 2 . Bologna, Edagricole.Google Scholar
Pons, T.L. and Schröder, H.F.J.M. (1986) Significance of temperature fluctuation and oxygen concentration for germination of the rice field weeds Fimbristylis littoralis and Scirpus juncoides . Oecologia 68, 315–319.Google Scholar
Puech, S., Ribannier, C. and Martin, A. (1997) Aerial seed bank and intra-population variability in germinative strategies of Verbascum phlomoides L. Plant Species Biology 12, 43–48.Google Scholar
Reinartz, J.A. (1984) Life history variation of common mullein (Verbascum thapsus). I. Latitudinal differences in population dynamics and timing of reproduction. Journal of Ecology 72, 897–912.Google Scholar
Remaley, T. (1998) Common mullein (Verbascum thapsus). Plant Conservation Alliance, Alien Plant Working Group, National Park Service. Available at http://www.nps.gov/plants/alien/fact/veth1.htm (accessed accessed 13 March 2003).Google Scholar
Rowse, H. and Finch-Savage, W. (2003) Hydrothermal threshold models can describe the germination response of carrot (Daucus carota) and onion (Allium cepa) seed populations across both sub- and supra-optimal temperatures. New Phytologist 158, 101–108.Google Scholar
Semenza, R.J., Young, J.A. and Evans, R.A. (1978) Influence of light and temperature on the germination and seedbed ecology of common mullein (Verbascum thapsus). Weed Science 26, 577–581.Google Scholar
Senel, E., Ozdener, Y. and Incedere, D. (2007) Effect of temperature, light, seed weight and GA3 on the germination of Verbascum bithynicum, Verbascum wiedemannianum and Salvia dicroantha . Pakistan Journal of Biological Sciences 10, 1118–1121.Google Scholar
Sileshi, G.W. (2012) A critique of current trends in the statistical analysis of seed germination and viability data. Seed Science Research 22, 145–159.Google Scholar
Thanos, C.A. and Doussi, M.A. (1995) Ecophysiology of seed germination in endemic labiates of Crete. Israel Journal of Plant Science 43, 227–237.Google Scholar
Thanos, C.A., Georghiou, K., Douma, D.J. and Marangaki, C.J. (1991) Photoinhibition of seed germination in Mediterranean maritime plants. Annals of Botany 68, 469–475.Google Scholar
Thompson, K. (1993) Seed persistence in soil. pp. 199–201 in Hendry, G.A.F.; Grime, J.P. (Eds) Methods in comparative plant ecology. London, Chapman & Hall.Google Scholar
Thompson, K. and Grime, J.P. (1979) Seasonal variation in the seed banks of herbaceous species in ten contrasting habitats. Journal of Ecology 67, 893–921.Google Scholar
Thompson, K. and Grime, J.P. (1983) A comparative study of germination responses to diurnally-fluctuating temperatures. Journal of Applied Ecology 20, 141–156.Google Scholar
Thompson, K., Grime, J.P. and Mason, G. (1977) Seed germination in response to diurnal fluctuations of temperature. Nature 267, 147–149.Google Scholar
Vázquez-Yanes, C. and Orozco-Segovia, A. (1994) Signals for seeds to sense and respond to gaps. pp. 209–236 in Caldwell, M.M.; Pearcy, R.W. (Eds) Exploitation of environmental heterogeneity by plants: Ecophysiological processes above and below ground. New York, Academic Press.Google Scholar
Yang, J., Lovett-Doust, J. and Lovett-Doust, L. (1999) Seed germination patterns in green dragon (Arisaema dracontium, Araceae). American Journal of Botany 86, 1160–1167.Google Scholar
Walck, J.L., Baskin, J.M. and Baskin, C.C. (2000) Increased sensitivity to green light during transition from conditional dormancy to nondormancy in seeds of three species of Solidago (Asteraceae). Seed Science Research 10, 495–499.Google Scholar
Wang, W.Q., Cheng, H.Y. and Song, S.Q. (2013) Development of a threshold model to predict germination of Populus tomentosa seeds after harvest and storage under ambient condition. PLoS ONE 8, e62868. doi:10.1371/journal.pone.006286 Google ScholarPubMed
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